Abstract:
:This communication describes a software tool that enables one to simulate large-scale regional mapping using an ordered shotgun sequencing approach. The analysis routines that are provided yield an estimate of the depth of coverage of the physical map, the largest contig formed, and the number of gaps remaining at any given juncture in the project. A detailed listing describing the span of each contig within the physical map is also presented. This provides an a priori means of estimating the resources that will be required to undertake any megabase mapping or sequencing project. CLONEPLACER provides the much needed guide to deriving the optimal strategy.
journal_name
Genomicsjournal_title
Genomicsauthors
Singh GB,Krawetz SAdoi
10.1016/0888-7543(95)80057-ssubject
Has Abstractpub_date
1995-01-20 00:00:00pages
555-8issue
2eissn
0888-7543issn
1089-8646pii
0888-7543(95)80057-Sjournal_volume
25pub_type
杂志文章相关文献
GENOMICS文献大全abstract::The genetic linkage of Chediak-Higashi syndrome and its murine analog, beige (bg), to the T-cell receptor (TCR-gamma) gamma chain gene is further defined. Previous studies using recombinant inbred strains of mice demonstrated that the murine bg gene is genetically linked to a murine TCR-gamma gene. We report that in t...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(87)90058-9
更新日期:1987-11-01 00:00:00
abstract::Here, we present the first complete genome sequence of brucellaphage Tbilisi (Tb) and compared it with that of Pr, a broad host-range brucellaphage recently isolated in Mexico. The genomes consist of 41,148 bp (Tb) and 38,253 bp (Pr), they differ mainly in the region encoding structural proteins, in which the genome o...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2012.01.001
更新日期:2012-04-01 00:00:00
abstract::Syndactyly in Holstein cattle is an autosomal recessive abnormality characterized by the fusion of the functional digits. This disorder has been previously mapped to the telomeric part of bovine chromosome 15. Here, we describe the fine-mapping of syndactyly in Holstein cattle to a 3.5-Mb critical interval using a com...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2006.05.007
更新日期:2006-11-01 00:00:00
abstract::The genomic structure, transcription start site, polyadenylation signals, and promoter of the human Na+/ myo-inositol cotransporter (SLC5A3) gene have been elucidated through cloning, sequencing, mRNA analyses, and reporter gene assays. The gene consists of one promoter and two exons spanning approximately 26 kb. Exon...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1997.5055
更新日期:1997-12-15 00:00:00
abstract::Type V collagen is a fibrillar collagen that is widely distributed in tissues as a minor component of extracellular matrix and is usually composed of one pro alpha 2 (V) and two pro alpha 1 (V) chains. In this report, recently isolated cDNA and genomic clones, which encode the pro alpha 1 (V) chain, are used as probes...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(92)90320-r
更新日期:1992-04-01 00:00:00
abstract::Full-length coding sequences of two novel human cadherin cDNAs were obtained by sequence analysis of several EST clones and 5' and 3' rapid amplification of cDNA ends (RACE) products. Exons for a third cDNA sequence were identified in a public-domain human genomic sequence, and the coding sequence was completed by 3' ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.2000.6305
更新日期:2000-09-15 00:00:00
abstract::To identify large proteins with an EGF-like-motif in a systematic manner, we developed a computer-assisted method called motif-trap screening. The method exploits 5'-end single-pass sequence data obtained from a pool of cDNAs whose sizes exceed 5 kb. Using this screening procedure, we were able to identify five known ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1998.5341
更新日期:1998-07-01 00:00:00
abstract::Familial dysplastic nevus syndrome (DNS) is an autosomal dominant premalignant condition characterized by multiple large moles of variable size and color and a strongly increased risk for cutaneous malignant melanoma. In order to determine the chromosomal localization of the DNS gene, linkage studies were initiated in...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(89)90086-4
更新日期:1989-07-01 00:00:00
abstract::Proteins with seven transmembrane segments (7TM) define a superfamily of receptors (7TM receptors) sharing the same topology: an extracellular N-terminus, three extramembranous loops on either side of the plasma membrane, and a cytoplasmic C-terminal tail. Upon ligand binding, cytoplasmic portions of the activated rec...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(95)80218-b
更新日期:1995-03-20 00:00:00
abstract::The ky mouse mutant, kyphoscoliosis, exhibits a degenerative muscle disease resulting in chronic deformation of the spinal column. Using an interspecific backcross segregating the ky mutation, we have mapped the ky locus to a small region of mouse chromosome 9. ky is nonrecombinant with the microsatellites D9Mit24 and...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(95)80127-8
更新日期:1995-01-01 00:00:00
abstract::We used targeted exon trapping to clone portions of genes from human chromosome 21q22.3. One trapped sequence showed complete homology with the cDNA of human U2AF35 (M96982; HGM-approved nomenclature U2AF1), which encodes for the small 35-kDa subunit of the U2 snRNP auxiliary factor. Using the U2AF1 cDNA as a probe, w...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1996.0196
更新日期:1996-04-15 00:00:00
abstract::Central to reconstruction of cis-regulatory networks is identification and classification of naturally occurring transcription factor-binding sites according to the genes that they control. We have examined salient characteristics of 9-mers that occur in various orders and combinations in the proximal promoters of hum...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2004.08.013
更新日期:2004-12-01 00:00:00
abstract::TM4SF11 is only 102 kb from the chemokine gene cluster composed of SCYA22, SCYD1, and SCYA17 on chromosome 16q13. CKLF maps on chromosome 16q22. CKLFs have some characteristics associated with the CCL22/MDC, CX3CL1/fractalkine, CCL17/TARC, and TM4SF proteins. Bioinformatics based on CKLF2 cDNA and protein sequences in...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/s0888-7543(03)00095-8
更新日期:2003-06-01 00:00:00
abstract::The behaviors of autism overlap with a diverse array of other neurological disorders, suggesting common molecular mechanisms. We conducted a large comparative analysis of the network of genes linked to autism with those of 432 other neurological diseases to circumscribe a multi-disorder subcomponent of autism. We leve...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2008.09.015
更新日期:2009-02-01 00:00:00
abstract::An exon representing a novel clathrin heavy chain gene (CLTCL) was isolated during gene identification studies and transcription mapping of human chromosome 22. Isolation and sequencing of cDNA clones corresponding to this exon revealed extensive similarity of the predicted amino acid sequence of this gene product to ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1996.0386
更新日期:1996-08-01 00:00:00
abstract::By direct sequence analysis of 94 mutant phenylalanine hydroxylase alleles using polymerase chain reaction-based techniques, we identified a C to T transition in exon 7 of the human phenylalanine hydroxylase gene that is associated with RFLP haplotypes 1 and 4. A leucine for proline substitution at position 281 can be...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(91)90238-a
更新日期:1991-01-01 00:00:00
abstract::Variation at MHC Class II-DQA locus in riverine and swamp buffaloes (Bubu) has been explored in this study. Through sequencing of buffalo DQA, 48 nucleotide variants identified from 17 individuals, reporting 42 novel alleles, including one pseudogene. Individual animal displayed two to seven variants, suggesting the p...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2020.07.041
更新日期:2020-11-01 00:00:00
abstract::There is a high level of conservation between human chromosomes and bovine syntenic groups. One such comparison is between human chromosome 12 and bovine chromosome 5, where at least 16 loci have been shown to be conserved in an homologous segment. However, the degree of conservation of order of the loci on bovine chr...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/s0888-7543(05)80280-0
更新日期:1992-09-01 00:00:00
abstract::Oncogenic hypophosphatemic osteomalacia (OHO) is characterized by a renal phosphate leak, hypophosphatemia, low-serum calcitriol (1,25-vitamin-D3), and abnormalities in skeletal mineralization. Resection of OHO tumors results in remission of the symptoms, and there is evidence that a circulating phosphaturic factor pl...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.2000.6235
更新日期:2000-07-01 00:00:00
abstract::The retina and its adjacent supporting tissues - retinal pigmented epithelium (RPE) and choroid - are critical structures in human eyes required for normal visual perception. Abnormal changes in these layers have been implicated in diseases such as age-related macular degeneration and glaucoma. With the advent of high...
journal_title:Genomics
pub_type: 杂志文章,评审
doi:10.1016/j.ygeno.2015.01.008
更新日期:2015-05-01 00:00:00
abstract::Specific probes derived from the human genes that complement the mutations of two independent temperature-sensitive (ts) mutants of the BHK-21 hamster cell line were used to determine the chromosomal locations of the loci in the human genome. The ts11 gene, which complements a mutation that blocks progression through ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(89)90326-1
更新日期:1989-04-01 00:00:00
abstract::Thirty-four random DNA probes from the terminal half of the human chromosome 4 short arm were further localized within 4pter----p15.1. A panel of somatic cell hybrid lines defining six chromosomal regions within 4pter----p15.1 was constructed using human cell lines containing translocation or deletion chromosomes. The...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(87)90101-7
更新日期:1987-09-01 00:00:00
abstract::The rice genome annotation has been greatly improved in recent years, largely due to the availability of full length cDNA sequences derived from many tissues. Among those yet to be studied is the aleurone layer, which produces hydrolases for mobilization of seed storage reserves during seed germination and post germin...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2013.10.007
更新日期:2014-01-01 00:00:00
abstract::PKD1, the gene for the chromosome 16-linked form of autosomal dominant polycystic kidney disease, has previously been genetically mapped to an interval bounded by the polymorphic loci Fr3-42/EKMDA2 distally and O327hb/O90a proximally. More recently, 26.6PROX was identified as the closest proximal flanking locus. We se...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(92)90215-e
更新日期:1992-05-01 00:00:00
abstract::Familial hemiplegic migraine (FHM) is a distinctive form of migraine with an autosomal dominant mode of inheritance. The migraine-like attacks are associated with transient hemiparesis. A locus for FHM has recently been assigned to chromosome 19 by linkage mapping. In the present study, five unrelated pedigrees with m...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1994.1340
更新日期:1994-07-01 00:00:00
abstract::The genomic structure of the mouse gene encoding the CDEBP protein has been established. The protein was initially identified on the basis of its ability to bind the CDEI motif (GTCACATG). The same locus has been independently described under the name APLP2, on the basis of sequence similarities with the Amyloid Precu...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1996.0318
更新日期:1996-07-01 00:00:00
abstract::The Kruppel-like factors (KLFs) belong to the family of zinc finger-containing transcription factors that regulates a diverse array of cellular processes, including cell proliferation, differentiation, and apoptosis. Here we reported the structure, mapping and phylogenetic analysis of KLF gene family in pigs. Comparat...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2009.11.001
更新日期:2010-02-01 00:00:00
abstract::The gene encoding activation-induced cytidine deaminase (AID), a member of the cytidine deaminase family, was isolated from a murine B cell lymphoma line, CH12F3-2, induced by combined stimulation of TGF-beta, IL-4, and CD40L. We have isolated the human orthologue of mouse AID cDNA, which has an open reading frame of ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.2000.6268
更新日期:2000-08-15 00:00:00
abstract::To predict the functions of a possible protein product of any new or uncharacterized DNA sequence, it is important first to detect all significant similarities between the encoded amino acid sequence and any accumulated protein sequence data. We have implemented a set of queries and database sequences and proceeded to...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1996.0614
更新日期:1996-12-01 00:00:00
abstract::The gene for prosaposin was characterized by sequence analysis of chromosomal DNA to gain insight into the evolution of this locus that encodes four highly conserved sphingolipid activator proteins or saposins. The 13 exons ranged in size from 57 to 1200 bp, while the introns were from 91 to 3812 bp in length. The reg...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(92)90247-p
更新日期:1992-06-01 00:00:00