Abstract:
:The genomic structure, transcription start site, polyadenylation signals, and promoter of the human Na+/ myo-inositol cotransporter (SLC5A3) gene have been elucidated through cloning, sequencing, mRNA analyses, and reporter gene assays. The gene consists of one promoter and two exons spanning approximately 26 kb. Exon 1 contains 175 bp of 5' untranslated sequence and is 15 kb upstream of exon 2. The 9.5-kb exon 2 contains the entire 2157-bp open reading frame and a large 3' untranslated sequence with seven putative polyadenylation signals. Multiple messages with different-sized 3' untranslated regions can be detected on Northern blots. Hypertonic stress caused mRNA levels, and primarily that of the full-length 9.5-kb transcript, to increase in cultured melanoma cells; ribonuclease protection analysis demonstrated that the transcription start site was the same in stressed as in control cells. The SLC5A3 gene functions in cellular osmoregulation and is expressed in many human tissues including the brain, kidney, and placenta. It is localized to chromosome 21q22.1. An overexpression of the SLC5A3 gene deserves consideration as a factor in the pathophysiology of Down syndrome.
journal_name
Genomicsjournal_title
Genomicsauthors
Mallee JJ,Atta MG,Lorica V,Rim JS,Kwon HM,Lucente AD,Wang Y,Berry GTdoi
10.1006/geno.1997.5055subject
Has Abstractpub_date
1997-12-15 00:00:00pages
459-65issue
3eissn
0888-7543issn
1089-8646pii
S0888-7543(97)95055-2journal_volume
46pub_type
杂志文章相关文献
GENOMICS文献大全abstract::A cDNA clone encoding the major antigen (CDR62) associated with the antibody-induced paraneoplastic cerebellar degeneration has been used to identify the chromosomal location of the corresponding structural gene(s) by screening for its retention in a panel of rodent-human somatic cell hybrids. Having established the s...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(92)90063-x
更新日期:1992-08-01 00:00:00
abstract::Helicobacter pylori is a Gram-negative spiral-shaped bacterium that infects half of the human population worldwide and causes chronic inflammation. In the present study, we used the art of computational biology for therapeutic drug targets identification and a multi-epitope vaccine against multi-strains of H. pylori. ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2020.06.026
更新日期:2020-09-01 00:00:00
abstract::We report the construction of a 1.5-Mb-resolution radiation hybrid map of the domestic cat genome. This new map includes novel microsatellite loci and markers derived from the 2X genome sequence that target previous gaps in the feline-human comparative map. Ninety-six percent of the 1793 cat markers we mapped have ide...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2006.08.007
更新日期:2007-02-01 00:00:00
abstract::We discuss the statistical significance of local similarities found between DNA sequences, and illustrate the procedure with reference to the Queen and Korn algorithm. If the longest similarity found for two sequences has length L, this length is said to be significant at the 5% level if there is a probability of no m...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(88)90081-x
更新日期:1988-10-01 00:00:00
abstract::In this study, selenocystine, a nutritionally available selenoamino acid, was identified for the first time as a novel agent with anti proliferative activity on human keloids. The 20 μM concentration after 48 h treatment used here was the most effective to reduce keloid fibroblast growth. We analyzed the gene expressi...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2011.02.009
更新日期:2011-05-01 00:00:00
abstract::Current microarray technology allows researchers to genotype a large number of SNPs with relatively small amounts of DNA. Nevertheless, researchers and clinicians still frequently face the problem of acquiring enough high-quality DNA for analysis. Whole-genome amplification (WGA) methods offer a solution for this prob...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2008.08.007
更新日期:2008-12-01 00:00:00
abstract::A somatic cell hybrid panel, consisting of 25 cell lines, has been developed to localize loci subregionally on chromosome 22. Over 300 markers in the form of STSs or hybridization probes have been assigned to one of 24 unique regions or "bins" using this panel. This ordered collection of markers will aid in the assemb...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1996.0358
更新日期:1996-07-15 00:00:00
abstract::Human Xq27 contains candidate regions for several disorders, yet is predicted to be a gene-poor cytogenetic band. We have developed a transcription map for the entire cytogenetic band to facilitate the identification of the relatively small number of expected candidate genes. Two approaches were taken to identify gene...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1999.5768
更新日期:1999-04-15 00:00:00
abstract::Sequence tag count-based gene expression analysis is potent for the identification of candidate genes relevant to the cancerous phenotype. With the public availability of count-based data, the computational approaches for differentially expressed genes, which are mainly based on Binomial or beta-Binomial distribution,...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2009.05.003
更新日期:2009-09-01 00:00:00
abstract::The TLE genes are the human homologues of Drosophila groucho, a member of the Notch signaling pathway. This pathway controls a number of different cell-fate choices in invertebrates and vertebrates. We are interested in investigating the functions of the TLE gene family during epithelial determination and carcinogenes...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1996.0009
更新日期:1996-01-01 00:00:00
abstract::The human ubiquitin-homology domain protein PIC1 interacts with the acute promyelocytic leukemia protein PML, and both proteins form part of the large, nuclear, multiprotein complexes known as PML nuclear bodies. The normal punctate immunohistochemical staining pattern of these complexes is disrupted by viral infectio...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1997.5091
更新日期:1998-01-01 00:00:00
abstract::RNA-seq is the method of choice for getting a primary list of genes for non-model organisms. Once this is achieved, one would proceed to annotate the newly discovered genes and consequently strive to position the organism in an evolutionary context. These kinds of studies involving high-throughput sequencing generate ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2014.12.004
更新日期:2015-03-01 00:00:00
abstract::TFE3, a member of the helix-loop-helix family of transcription factors, binds to the microE3 motif of the immunoglobulin heavy-chain enhancer and is expressed in many cell types. We have localized human TFE3 to the proximal short arm of the X chromosome using a somatic cell hybrid panel. A frequent RsaI RFLP detected ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(91)90145-5
更新日期:1991-10-01 00:00:00
abstract::The avian embryo develops within a specialized biological container (eggshell) that contains crucial nutritional compartments (albumen, yolk). We analyzed the transcriptome of ovary and three segments of oviduct, including magnum, isthmus and uterus in the chicken during egg formation. RNA-Seq libraries (42 in total) ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2019.02.003
更新日期:2020-01-01 00:00:00
abstract::We completed a systematic survey of the human genome, conducted at an average resolution of 10 cM, for the identification of simple sequence tandem repeat polymorphisms (SSTRPs) that target new risk genes for Alzheimer disease (AD) by virtue of linkage disequilibrium. The efficiency of our association study was enhanc...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1998.5306
更新日期:1998-06-01 00:00:00
abstract::This paper describes the Centre d'Etude du Polymorphisme Humain (CEPH) consortium linkage map of chromosome 9. A total of 124 markers were typed in the CEPH family DNAs by 14 contributing laboratories; of these, 42 loci are ordered on the map with likelihood support of at least 1000:1. The uniquely placed markers incl...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1994.1049
更新日期:1994-01-15 00:00:00
abstract::The transcriptional organization of the region of the mouse X chromosome between the G6pd and the Fln1 genes was studied in detail, and it was compared with the syntenic region of the human chromosome. A cosmid contig of 250 kb was constructed by screening mouse cosmid libraries with probes for human genes and with wh...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1995.1164
更新日期:1995-08-10 00:00:00
abstract::The human gene XPC (formerly designated XPCC), which corrects the repair deficiency of xeroderma pigmentosum (XP) group C cells, was mapped to 3p25. A cDNA probe for Southern blot hybridization and diagnostic PCR analyses of hybrid clone panels informative for human chromosomes in general and portions of chromosome 3 ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1994.1256
更新日期:1994-05-01 00:00:00
abstract::A key challenge in analyzing metagenomics data pertains to assembly of sequenced DNA fragments (i.e. reads) originating from various microbes in a given environmental sample. Several existing methodologies can assemble reads originating from a single genome. However, these methodologies cannot be applied for efficient...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2014.02.007
更新日期:2014-02-01 00:00:00
abstract::Consecutive application of PCR and serial analysis of gene expression (SAGE) was used to generate a catalog of approximately 50, 000 SAGEtags from nine human oocytes. Matches for known genes were identified using the National Institutes of Health SAGEtag database. This database links directly to the UniGene database, ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1999.6059
更新日期:2000-01-01 00:00:00
abstract::Portions of 16 chromosome 21 NotI linking clones were sequenced. These linking clone sequences represent sequence-tagged restriction sites that are potentially useful for finding genes and for finer genome mapping and sequencing. All of the clones were G+C rich (54 to 83%). CpG and GpC dinucleotide frequencies were ve...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1993.1455
更新日期:1993-11-01 00:00:00
abstract::The human HYAL2 gene encodes a lysosomal hyaluronidase that is related to the testicular PH-20 hyaluronidase. Regions conserved in these proteins have been used to design PCR primers suitable for the isolation of a fragment of the murine Hyal2 gene. This fragment was used to isolate the Hyal2 cDNA from a cDNA library....
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1998.5472
更新日期:1998-10-15 00:00:00
abstract::Interspecific somatic cell hybrids containing single human chromosomes are valuable reagents for localization of cloned genes and DNA fragments to specific chromosomes, for the development of chromosome-specific libraries, and for generation of hybrid cell lines containing subchromosomal regions. A CHO somatic cell hy...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(91)90122-u
更新日期:1991-09-01 00:00:00
abstract::Indolethylamine N-methyltransferase (INMT) catalyzes the N-methylation of tryptamine and structurally related compounds. We recently cloned and characterized the rabbit INMT cDNA and gene as a step toward cloning the cDNA and gene for this enzyme in humans. We have now used a PCR-based approach to clone a human INMT c...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1999.5960
更新日期:1999-11-01 00:00:00
abstract::To facilitate studies of the SRY gene, a 4741-bp portion of the sex-determining region of the human Y chromosome was sequenced and characterized. Two RNAs were found to hybridize to this genomic segment, one transcript deriving from SRY and the second cross-hybridizing to a pseudogene located 2.5 kb 5' of the SRY open...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1993.1395
更新日期:1993-09-01 00:00:00
abstract::The desmogleins, together with the desmocollins, both members of the cadherin superfamily, are the adhesive proteins of the desmosome type of cell junction, characteristically found in epithelial cells. Three different human desmoglein isoforms are encoded by separate genes (DSG1, DSG2, and DSG3) located on chromosome...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(95)80067-v
更新日期:1995-01-20 00:00:00
abstract::This communication describes a software tool that enables one to simulate large-scale regional mapping using an ordered shotgun sequencing approach. The analysis routines that are provided yield an estimate of the depth of coverage of the physical map, the largest contig formed, and the number of gaps remaining at any...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(95)80057-s
更新日期:1995-01-20 00:00:00
abstract::Prostaglandin EP3 receptor subtype is a seven-membrane-spanning protein with multiple C-terminal tails generated by alternative mRNA splicing. We report here the structural organization of the human EP3 gene (PTGER3). The human EP3 gene spanned more than 80 kb and was composed of 10 exons separated by nine introns. Ex...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1996.4585
更新日期:1997-03-15 00:00:00
abstract::Phenol- and monoamine-metabolizing sulfotransferases (STP and STM, respectively) are members of a superfamily of enzymes that add sulfate to a variety of xenobiotics and endobiotics containing hydroxyl or amino functional groups. To characterize related sulfotransferase genes further, we used extra-long PCR (XL-PCR) t...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1996.4575
更新日期:1997-03-01 00:00:00
abstract::The Friedreich's ataxia locus has been previously assigned to chromosome 9q 13-21.1 by the demonstration of tight linkage to two anonymous DNA markers. MCT112 (Z greater than 80, theta = 0) and DR47 (Z greater than 50, theta = 0). The absence of recombination between these three loci has prevented the resolution of ge...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(91)90224-3
更新日期:1991-01-01 00:00:00