Abstract:
:Indolethylamine N-methyltransferase (INMT) catalyzes the N-methylation of tryptamine and structurally related compounds. We recently cloned and characterized the rabbit INMT cDNA and gene as a step toward cloning the cDNA and gene for this enzyme in humans. We have now used a PCR-based approach to clone a human INMT cDNA that had a 792-bp open reading frame that encoded a 263-amino-acid protein 88% identical in sequence to rabbit INMT. Northern blot analysis of 35 tissues showed that a 2.7-kb INMT mRNA species was expressed in most tissues. When the cDNA was expressed in COS-1 cells, the recombinant enzyme catalyzed the methylation of tryptamine with an apparent K(m) value of 2.9 mM. The human cDNA was then used to clone the human INMT gene from a human genomic BAC library. The gene was 5471 bp in length, consisted of three exons, and was structurally similar to the rabbit INMT gene as well as genes for nicotinamide N-methyltransferase and phenylethanolamine N-methyltransferase in several species. All INMT exon-intron splice junctions conformed to the "GT-AG" rule, and no canonical TATA or CAAT sequences were present within the 5'-flanking region of the gene. Human INMT mapped to chromosome 7p15.2-p15.3 on the basis of both PCR analysis and fluorescence in situ hybridization. Finally, two possible single nucleotide polymorphisms were identified within exon 3, both of which altered the encoded amino acid. The cloning and expression of a human INMT cDNA, as well as the cloning, structural characterization, and mapping of its gene represent steps toward future studies of the function and regulation of this methyltransferase enzyme in humans.
journal_name
Genomicsjournal_title
Genomicsauthors
Thompson MA,Moon E,Kim UJ,Xu J,Siciliano MJ,Weinshilboum RMdoi
10.1006/geno.1999.5960subject
Has Abstractpub_date
1999-11-01 00:00:00pages
285-97issue
3eissn
0888-7543issn
1089-8646pii
S0888-7543(99)95960-8journal_volume
61pub_type
杂志文章相关文献
GENOMICS文献大全abstract::Knowledge of protein subcellular localization is vitally important for both basic research and drug development. With the avalanche of protein sequences emerging in the post-genomic age, it is highly desired to develop computational tools for timely and effectively identifying their subcellular localization purely bas...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2018.05.017
更新日期:2019-07-01 00:00:00
abstract::We have previously described the cDNA coding for a new lipoprotein receptor that contains domains closely related to the ligand-binding domain of the LDL receptor. We have now investigated the localization of the gene for this new receptor by hybridization of the cDNA to panels of rodent cells containing subsets of hu...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(89)90087-6
更新日期:1989-07-01 00:00:00
abstract::Sogatella furcifera is an important rice pest. In order to understand the molecular basis of the sex determination in this pest, we performed de novo transcriptome sequencing of six cDNA libraries (three biological replicates) of female and male fifth-instar nymphs. Total 65,199 unigenes were obtained, with an average...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2019.06.024
更新日期:2020-03-01 00:00:00
abstract::Differential epigenetic modification by methylation of CpG dinucleotides is a candidate mechanism that may identify the alleles of imprinted genes and result in monoallelic expression of either the maternal or the paternal allele. Determination of the allelic methylation status of imprinted genes in the gametes and du...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1998.5570
更新日期:1998-12-15 00:00:00
abstract::The human MAGE1 gene directs the expression of an antigen recognized on a melanoma by autologous cytolytic T lymphocytes. MAGE1 belongs to a family of genes that are expressed in a number of tumors of various histological types but not in normal tissues except testis. The MAGE genes are arranged in two groups that are...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1995.1108
更新日期:1995-07-01 00:00:00
abstract::We have developed a high-information-content fingerprinting (HICF) system for bacterial artificial chromosome (BAC) clones using a Type IIS restriction endonuclease, HgaI, paired with a Type II restriction endonuclease, RsaI. In the method described, unknown five-base overhangs generated with HgaI are partially or ful...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.2001.6547
更新日期:2001-06-01 00:00:00
abstract::DNA 5' to the human myelin basic protein (MBP) gene, mapped to 18q22----qter, is known to manifest multiallelic DNA length variation with heterozygosity of at least 45%. Isolation of genomic DNA containing the MBP gene first exon and its 5' flanking region reveals that this polymorphism arises from a 994-bp region of ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(90)90443-x
更新日期:1990-01-01 00:00:00
abstract::A large insert canine genomic bacterial artificial chromosome (BAC) library was built from a Doberman pinscher. Approximately 166,000 clones were gridded on nine high-density hybridization filters. Insert analysis of randomly selected clones indicated a mean insert size of 155 kb and predicted 8.1 coverage of the cani...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1999.5772
更新日期:1999-05-15 00:00:00
abstract::The lack of physical mapping data strongly restricts the analysis of the meningioma chromosomal region that was assigned to the bands 22q12.3-qter. Recently, we reported a new marker D22S16 for chromosome 22 that was assigned to the region 22q13-qter by in situ hybridization. Utilizing somatic cell hybrids we now subl...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(91)90196-l
更新日期:1991-08-01 00:00:00
abstract::To investigate the molecular mechanism of silkworm resistance to BmNPV infection, we constructed a near-isogenic line (BC8) with BmNPV resistance using highly resistant (NB) and highly susceptible parental strains (306). We investigated variations in the gene expression in the midguts of BmNPV-infected BC8 and 306 at ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2013.02.004
更新日期:2013-04-01 00:00:00
abstract::Cross-species comparative genomics is a powerful strategy for identifying functional regulatory elements within noncoding DNA. In this paper, comparative analysis of human and mouse intronic sequences in the breast cancer susceptibility gene (BRCA1) revealed two evolutionarily conserved noncoding sequences (CNS) in in...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2005.05.006
更新日期:2005-09-01 00:00:00
abstract::A population of chromosome segment substitution lines was developed using KDML105 as the recurrent parent and one of DH212 (IR68586-F2-CA-143) or DH103 (IR68586-F2-CA-31) as the donor parent. The donor parents are part of a doubled haploid population from a cross between CT9993, an upland japonica accession, and IR622...
journal_title:Genomics
pub_type: 杂志文章,评审
doi:10.1016/j.ygeno.2019.10.001
更新日期:2019-10-31 00:00:00
abstract::The genes encoding the alpha 1 chain of Type III collagen (COL3A1) and the alpha 2 chain of Type V (COL5A2) collagen have been mapped to the long arm of human chromosome 2. Linkage analysis in CEPH families indicated that these two genes are close to each other, with no recombination in 37 informative meioses. In the ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(90)90302-b
更新日期:1990-10-01 00:00:00
abstract::Chemotherapeutic response of cancer cells to a given compound is one of the most fundamental information one requires to design anti-cancer drugs. Recently, considerable amount of drug-induced gene expression data has become publicly available, in addition to cytotoxicity databases. These large sets of data provided a...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2018.07.002
更新日期:2019-09-01 00:00:00
abstract::Based on its map position, polymorphism pattern, and expression in the kidney, the gene encoding liver 20,000-30,000 MW protein 4 (LTW4) can be considered a potential candidate for the Jckm2 modifying locus, which mediates the severity of polycystic kidney disease in the juvenile cystic kidney mouse. Using two-dimensi...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1997.4762
更新日期:1997-06-15 00:00:00
abstract::Physical maps can be constructed by "fingerprinting" a large number of random clones and inferring overlap between clones when the fingerprints are sufficiently similar. E. Lander and M. Waterman (Genomics 2: 231-239, 1988) gave a mathematical analysis of such mapping strategies. The analysis is useful for comparing v...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(95)80086-2
更新日期:1995-03-01 00:00:00
abstract::We have analyzed a dense set of single-nucleotide polymorphisms (SNPs) and microsatellites spanning the T-helper cytokine gene cluster (interleukins 3, 4, 5, 9, and 13, interferon regulatory factor-1, colony-stimulating factor-2, and T-cell transcription factor-7) on 5q31 and the gene encoding the interleukin-4 recept...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.2001.6613
更新日期:2001-09-01 00:00:00
abstract::Using a slot-blot method for the dosage of single-copy sequences, the copy numbers of 30 chromosome 21 markers were assessed in the blood DNA of 11 patients with partial trisomy or monosomy 21 and in the DNA of a patient-derived human-hamster hybrid cell line carrying a microduplication of chromosome 21. The physical ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(92)90177-t
更新日期:1992-07-01 00:00:00
abstract::Sequence tag count-based gene expression analysis is potent for the identification of candidate genes relevant to the cancerous phenotype. With the public availability of count-based data, the computational approaches for differentially expressed genes, which are mainly based on Binomial or beta-Binomial distribution,...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2009.05.003
更新日期:2009-09-01 00:00:00
abstract::We used a combination of sequence analysis and exon trapping in an effort to determine the complete transcript map for a cosmid (6E5) derived from 12q13.3, a region of DNA sequence amplification in human cancers. This cosmid, previously known to contain three genes (CDK4, SAS, and OS9), was sequenced, and that informa...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1997.4727
更新日期:1997-06-01 00:00:00
abstract::A new Alcanivorax sp. VBW004 was isolated from a shallow hydrothermal vent in Azores Island, Portugal. In this study, we determined VBW004 was resistant to copper. This strain showed maximum tolerance of copper concentrations up to 600 μg/mL. Based on 16S rRNA gene sequencing and phylogeny revealed that this strain wa...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2020.06.015
更新日期:2020-09-01 00:00:00
abstract::The rat diabetes susceptibility gene, Lyp or Lymphopenia, has been localized to RNO4. Proximal to Lyp are the genes caspase-2 (Casp2) and pancreatic trypsin 1 (Prss1), while neuropeptide Y (Npy) is the closest distally positioned gene. In human, the three genes are syntenic on HSA7, but they are not on a conserved seg...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.2000.6130
更新日期:2000-04-01 00:00:00
abstract::We present a novel method, based on the hybridization of allele-specific oligonucleotide probes, that allows the specific detection of chromosome 21 alpha-satellite sequences. Absence of informative polymorphic markers from the centromeric region of chromosome 21 has constituted one of the difficulties in studying the...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1999.5786
更新日期:1999-05-01 00:00:00
abstract::Members of the apolipoprotein gene cluster (APOA1/C3/A4/A5) on human chromosome 11q23 play an important role in lipid metabolism. Polymorphisms in both APOA5 and APOC3 are strongly associated with plasma triglyceride concentrations. The close genomic locations of these two genes as well as their functional similarity ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2003.11.016
更新日期:2004-05-01 00:00:00
abstract::We have used probes from the human genes PI, PIL, and AACT (alpha 1-antitrypsin, alpha 1-antitrypsin-related sequence, and alpha 1-antichymotrypsin) to make a pulsed-field map of the surrounding region of 14q31-32. We have discovered that the PI-PIL gene cluster is only 220 kb away from the AACT gene and that it is or...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(90)90172-q
更新日期:1990-07-01 00:00:00
abstract::The large number of redundant sequences available in nucleotide databases provides a resource for the identification of polymorphisms. Expressed polymorphisms in X-linked genes can be used to determine the inactivation status of the genes, and polymorphisms in genes that are subject to inactivation can then be used as...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.2000.6153
更新日期:2000-04-01 00:00:00
abstract::Aspartylglucosaminuria (AGU) is a recessively inherited lysosomal disease caused by inadequate aspartylglucosaminidase (AGA) activity. The disease is prevalent in the genetically isolated Finnish population. We have used a new method, solid-phase minisequencing, to determine the frequency of two missense mutations in ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(92)90452-x
更新日期:1992-03-01 00:00:00
abstract::Human myosin light chain-2 (MYL2) is an important protein involved in the regulation of myosin ATPase activity in smooth muscle. In cardiac muscle, the precise role of MYL2 is not well understood; however, an increase in ventricular MYL2 is observed during myocardial hypertrophy in cardiac patients with valve stenosis...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(92)90161-k
更新日期:1992-07-01 00:00:00
abstract::Parasites of the genus Plasmodium infect a wide array of hosts, causing malaria in all major groups of terrestrial vertebrates including primates, reptiles, and birds. Molecular mechanisms explaining why some Plasmodium species are virulent, while other closely related malaria pathogens are relatively benign in the sa...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2018.12.004
更新日期:2019-12-01 00:00:00
abstract::The Long-Evans Cinnamon (LEC) rat has a spontaneous mutation, T helper immunodeficiency (thid), which causes a markedly reduced CD4(+) thymocyte population. Here we positionally clone the locus and identify a deletion in the gene encoding a receptor-like protein tyrosine phosphatase kappa (Ptprk) that led to complete ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2007.03.001
更新日期:2007-06-01 00:00:00