Abstract:
:Human myosin light chain-2 (MYL2) is an important protein involved in the regulation of myosin ATPase activity in smooth muscle. In cardiac muscle, the precise role of MYL2 is not well understood; however, an increase in ventricular MYL2 is observed during myocardial hypertrophy in cardiac patients with valve stenosis. The chromosomal location of the gene coding for MYL2 was identified using a cloned cDNA for human MYL2. Southern blot analysis of DNA from a human/rodent somatic cell hybrid mapping panel showed that the BamHI fragment that hybridized with this cDNA probe was concordant with chromosome 12. The 768-bp cDNA was hybridized to human metaphase chromosomes. The results revealed a significant clustering of silver grains over chromosome 12 bands q23-q24.3, indicating that the gene coding for MYL2 is located in this region.
journal_name
Genomicsjournal_title
Genomicsauthors
Macera MJ,Szabo P,Wadgaonkar R,Siddiqui MA,Verma RSdoi
10.1016/0888-7543(92)90161-ksubject
Has Abstractpub_date
1992-07-01 00:00:00pages
829-31issue
3eissn
0888-7543issn
1089-8646pii
0888-7543(92)90161-Kjournal_volume
13pub_type
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