Abstract:
:Transcripts related to the human carcinoembryonic antigen were found in mRNA isolated from both dimethylbenzanthracene-induced and mouse mammary tumor virus-induced mammary tumors. A cDNA library was prepared from a dimethylbenzanthracene-induced tumor, and a clone was isolated by hybridization with a human carcinoembryonic antigen cDNA probe. Its sequence, when compared to those of members of the human carcinoembryonic antigen gene family, was most homologous to the sequence of the human biliary glycoprotein (BGP) gene. Thus, this clone appears to encode a portion of the mouse biliary glycoprotein gene. Southern blot analysis of EcoRI-digested mouse cellular DNA with this probe detected four restriction fragments, all of which appear to be located on mouse chromosome 7, Northern blot analysis using the mouse probe demonstrated that related mRNA species were expressed in some normal adult mouse tissues.
journal_name
Genomicsjournal_title
Genomicsauthors
Robbins J,Robbins PF,Kozak CA,Callahan Rdoi
10.1016/0888-7543(91)90439-lsubject
Has Abstractpub_date
1991-07-01 00:00:00pages
583-7issue
3eissn
0888-7543issn
1089-8646journal_volume
10pub_type
杂志文章相关文献
GENOMICS文献大全abstract::Human myosin light chain-2 (MYL2) is an important protein involved in the regulation of myosin ATPase activity in smooth muscle. In cardiac muscle, the precise role of MYL2 is not well understood; however, an increase in ventricular MYL2 is observed during myocardial hypertrophy in cardiac patients with valve stenosis...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(92)90161-k
更新日期:1992-07-01 00:00:00
abstract::Cystathionine beta-synthase [CBS; l-serine hydro-lyase (adding homocysteine), EC 4.2.1.22] catalyzes the first committed step of transsulfuration and is the enzyme deficient in classical homocystinuria. In this report, we describe the molecular cloning and the complete nucleotide sequence of the human CBS gene. We rep...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1998.5437
更新日期:1998-09-15 00:00:00
abstract::Perilipin 1 (PLIN1) protein, also known as lipid droplet-associated protein, is encoded by the PLIN1 gene and is able to anchor itself to the membranes of lipid droplets. The phosphorylation of PLIN1 is critical for the mobilization of fat in adipose tissue and plays an important role in regulating lipolysis and lipid...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2020.01.012
更新日期:2020-05-01 00:00:00
abstract::A key challenge in analyzing metagenomics data pertains to assembly of sequenced DNA fragments (i.e. reads) originating from various microbes in a given environmental sample. Several existing methodologies can assemble reads originating from a single genome. However, these methodologies cannot be applied for efficient...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2014.02.007
更新日期:2014-02-01 00:00:00
abstract::Cardiac hypertrophy is an important risk factor for cardiac morbidity and mortality. To unravel the underlying pathogenic genetic pathways, we hybridized left ventricular RNA from Transverse Aortic Constriction mice at 48 h, 1 week, and 2, 3, and 8 weeks after surgery to microarrays containing a 15K fetal cDNA collect...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2006.04.012
更新日期:2006-10-01 00:00:00
abstract::The single functional mouse gene for MIF (macrophage migration inhibitory factor) has been cloned from a P1 library, and its exon/intron structure determined and shown to resemble that of the human gene. The gene was mapped to chromosome 10 using two multilocus crosses between laboratory strains and either Mus musculu...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1995.1070
更新日期:1995-06-10 00:00:00
abstract::We isolated the human homologue, SUPT5H, of the yeast transcription factor, SPT5. The human homologue is 1088 aa long compared to 1063 aa for the yeast gene. SUPT5H maps to 19q13, near the ryanodine receptor. Like its family member, SUPT6H, and like yeast SPT5, SUPT5H has a very acidic 5' domain. Like its family membe...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1996.0646
更新日期:1996-12-15 00:00:00
abstract::Zebrafish transgenesis is a powerful and increasingly common strategy to assay vertebrate transcriptional regulatory control. Several challenges remain, however, to the broader application of this technique; they include increasing the rate with which transgenes can be analyzed and maximizing the informational value o...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2010.02.011
更新日期:2010-06-01 00:00:00
abstract::In humans, uridine 5'-diphosphate glucuronosyltransferase (UGT) operates in opposition to glucuronidase (GUS) to control activity of diverse metabolites such as hormones by reversible conjugation with glucuronic acid. Previous data revealed that, as in mammals, these enzymes are required for plant life in that a UGT f...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2007.03.014
更新日期:2007-07-01 00:00:00
abstract::We present a novel method, based on the hybridization of allele-specific oligonucleotide probes, that allows the specific detection of chromosome 21 alpha-satellite sequences. Absence of informative polymorphic markers from the centromeric region of chromosome 21 has constituted one of the difficulties in studying the...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1999.5786
更新日期:1999-05-01 00:00:00
abstract::Most members of the large family of rhodopsin-like G-protein-coupled receptors possess an evolutionarily conserved Asp-Arg-Tyr (DRY) motif in the C-terminal region of the third transmembrane domain. Mutations of residues within this motif usually abolish receptor function and, when they occur naturally, can even cause...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2006.02.009
更新日期:2006-06-01 00:00:00
abstract::Neurofibromatosis 1 maps to chromosome band 17q11.2, and the NF1 locus has been partially characterized. Even though the full-length NF1 cDNA has been sequenced, the complete genomic structure of the NF1 gene has not been elucidated. The 5' end of NF1 is embedded in a CpG island containing a NotI restriction site, and...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(95)80104-t
更新日期:1995-01-01 00:00:00
abstract::Differentiation of Wharton's Jelly-Mesenchymal Stem cells (WJ-MSCs) into cardiomyocytes (CMs) in vitro has been reported widely although contradictions remain regarding the maturation of differentiated MSCs into fully functioning CMs. Studies suggest that use of epigenetic modifiers like 5'Azacytidine (5-AC) in MSCs d...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2019.08.007
更新日期:2020-03-01 00:00:00
abstract::A cDNA encoding the human GABAA receptor beta 3 subunit has been isolated from a brain cDNA library and its nucleotide sequence has been determined. This gene, GABRB3, has recently been mapped to human chromosome 15q11q13, the region deleted in Angelman and Prader-Willi syndromes. The association of distinct phenotype...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(91)90034-c
更新日期:1991-12-01 00:00:00
abstract::Two murine homologs of the Drosophila Krüppel gene, a member of the gap class of developmental control genes that encode a protein with zinc fingers, were mapped to mouse chromosomes 8 and 11 by using somatic cell hybrids and an interspecific backcross. Surprisingly, both genes were closely linked to two previously ma...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(90)90033-q
更新日期:1990-11-01 00:00:00
abstract::Most parasitic flatworms go through different life stages with important physiological and morphological changes. In this work, we used a transcriptomic approach to analyze the main life-stages of the model tapeworm Hymenolepis microstoma (eggs, cysticercoids, and adults). Our results showed massive transcriptomic cha...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2021.01.005
更新日期:2021-01-21 00:00:00
abstract::End sequences from bacterial artificial chromosomes (BACs) provide highly specific sequence markers in large-scale sequencing projects. To date, we have generated >300,000 end sequences from >186,000 human BAC clones with an average read length of >460 bp for a total of 141 Mb covering approximately 4.7% of the genome...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1999.6082
更新日期:2000-02-01 00:00:00
abstract::An alarming increase in the human population necessitates doubling the world food production in the next few decades. Although a number of possible biotechnological measures are under consideration, central to these efforts is the development of transgenic crops to produce more food, and the traits with which plants c...
journal_title:Genomics
pub_type: 杂志文章,评审
doi:10.1016/j.ygeno.2017.07.007
更新日期:2017-10-01 00:00:00
abstract::The human genes encoding the alpha and beta forms of the retinoic acid receptor are known to be located on chromosomes 17 (band q21.1:RARA) and 3 (band p24:RARB). By in situ hybridization, we have now localized the gene for retinoic acid receptor gamma, RARG, on chromosome 12, band q13. We also mapped the three retino...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(91)90199-o
更新日期:1991-08-01 00:00:00
abstract::Genes that encode the vertebrate fibrillar collagen types I-III have previously been shown to share a highly conserved intron/exon organization, thought to reflect common ancestry and evolutionary pressures at the protein level. We report here the complete intron/exon organization of COL5A1, the human gene that encode...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1995.9961
更新日期:1995-10-10 00:00:00
abstract::The sequence of the human Gc gene, including 4228 base pairs of the 5'-flanking region and 8514 base pairs of the 3' flanking region (55,136 in total), was determined from five overlapping lambda phage clones. The sequence spans 42,394 base pairs from the cap site to the polyadenylation site, and it reveals that the g...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1993.1258
更新日期:1993-06-01 00:00:00
abstract::Using an interspecies backcross, we have mapped the HOX-5 and surfeit (surf) gene clusters within the proximal portion of mouse chromosome 2. While the HOX-5 cluster of homeobox-containing genes has been localized to chromosome 2, bands C3-E1, by in situ hybridization, its more precise position relative to the genes a...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(90)90499-k
更新日期:1990-04-01 00:00:00
abstract::Type V collagen is a fibrillar collagen that is widely distributed in tissues as a minor component of extracellular matrix and is usually composed of one pro alpha 2 (V) and two pro alpha 1 (V) chains. In this report, recently isolated cDNA and genomic clones, which encode the pro alpha 1 (V) chain, are used as probes...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(92)90320-r
更新日期:1992-04-01 00:00:00
abstract::Retinitis pigmentosa (RP) is a heterogeneous genetic disorder with autosomal dominant, autosomal recessive, and X-linked forms. We previously mapped an additional arRP locus to chromosome 6p21 (RP14) in a single extended kinship from the Dominican Republic. Aided by a second linked RP pedigree from the same region of ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1997.5174
更新日期:1998-03-01 00:00:00
abstract::Human chromosome 11 harbors many genes of medical significance and cancer-related rearrangements. The availability of cloned DNA in cosmids and in yeast artificial chromosomes (YACs), combined with fluorescence in situ hybridization analysis, has led to the cloning of genes at sites of chromosomal breakpoints in acute...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1994.1354
更新日期:1994-07-01 00:00:00
abstract::In an attempt to identify the genetic basis for susceptibility to non-insulin-dependent diabetes mellitus within the context of obesity, we generated 401 genetically obese Leprfa/Leprfa F2 WKY13M intercross rats that demonstrated wide variation in multiple phenotypic measures related to diabetes, including plasma gluc...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1997.4672
更新日期:1997-05-01 00:00:00
abstract::Cyclin-dependent kinase 5 (Cdk5) is predominantly expressed in neurons. In vitro, Cdk5 purified from the nervous tissue phosphorylates both high-molecular-weight neurofilament and microtubule-associated tau. The mouse gene encoding Cdk5 (Cdk5) was found to be 5 kb in length and divided into 12 exons. All of the exon-i...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1995.1194
更新日期:1995-08-10 00:00:00
abstract::To investigate the molecular mechanism of silkworm resistance to BmNPV infection, we constructed a near-isogenic line (BC8) with BmNPV resistance using highly resistant (NB) and highly susceptible parental strains (306). We investigated variations in the gene expression in the midguts of BmNPV-infected BC8 and 306 at ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2013.02.004
更新日期:2013-04-01 00:00:00
abstract::Despite antibiotic therapy and vaccination programs, microbial diseases continue to be the leading cause of morbidity and mortality worldwide. The genetic basis of the host response to infection is complex, and its understanding has been facilitated through the study of mouse models of human infectious diseases. Genet...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1997.5116
更新日期:1998-01-15 00:00:00
abstract::Long sequencing reads offer unprecedented opportunities in analysis and reconstruction of complex genomic regions. However, the gain in sequence length is often traded for quality. Therefore, recently several approaches have been proposed (e.g. higher sequencing coverage, hybrid assembly or sequence correction) to enh...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2017.12.011
更新日期:2019-01-01 00:00:00