Abstract:
:Expression of p107, a protein with homology to the retinoblastoma tumor suppressor (pRB), was monitored during murine development. Northern blot tissue surveys identified two transcripts of 4.9 and 2.4 kb that hybridized to a p107 cDNA clone. Expression of both transcripts was detected in fetal tissues, with particularly high levels in the liver and heart. In contrast, p107 transcripts were markedly decreased in most adult tissues examined. Molecular cloning analyses revealed that the 4.9- and 2.4-kb transcripts encoded proteins with deduced molecular masses of 119 and 68 kDa, respectively. Genetic mapping studies suggested that the two p107 transcripts arose by alternative splicing of a common precursor. The protein encoded by the 2.4-kb transcript lacks the spacer and B motif of the "pocket domain," a region of homology between p107 and pRB that is required for binding to cell cycle regulatory proteins. Structural modifications resulting from alternative splicing may thus confer functional diversity upon the 119- and 68-kDa proteins.
journal_name
Genomicsjournal_title
Genomicsauthors
Kim KK,Soonpaa MH,Wang H,Field LJdoi
10.1006/geno.1995.1184subject
Has Abstractpub_date
1995-08-10 00:00:00pages
520-9issue
3eissn
0888-7543issn
1089-8646pii
S0888-7543(85)71184-6journal_volume
28pub_type
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