Abstract:
:In this paper we describe a method that uses the nearly covalent strength biotin-streptavidin interaction to attach a paramagnetic bead of micrometer size to a DNA molecule of nanometer size, scaling up the spatial size of a query DNA strand by a factor of 1000, making it visible to the human eye. The use of magnetic principles enables rapid binding and washing of detector beads, facilitating a readout of amplified DNA sequences in a few minutes. Here we exemplify the method on mitochondrial DNA variations using an array platform. Visual identification and documentation can be performed with an ordinary mobile phone equipped with a built-in camera.
journal_name
Genomicsjournal_title
Genomicsauthors
Ståhl PL,Gantelius J,Natanaelsson C,Ahmadian A,Andersson-Svahn H,Lundeberg Jdoi
10.1016/j.ygeno.2007.07.014subject
Has Abstractpub_date
2007-12-01 00:00:00pages
741-5issue
6eissn
0888-7543issn
1089-8646pii
S0888-7543(07)00197-8journal_volume
90pub_type
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