Abstract:
:Spt3 is a Saccharomyces cerevisiae transcription factor that is required in vivo for the transcription of a number of RNA polymerase II-transcribed genes. We report the cloning of the gene encoding the human homologue of Spt3, SUPT3H, and its initial functional analysis. The human and yeast Spt3 homologues share an overall identity of 30% that defines three conserved regions, suggesting possible functional domains. To determine whether SUPT3H is a true functional Spt3 homologue, we tested for complementation of an spt3Delta mutation in yeast. While expression of the full-length SUPT3H is unable to complement an spt3Delta mutation, expression of a human-yeast chimeric gene that contains 42% human sequences can partially complement an spt3Delta mutation. These data suggest that the transcriptional control carried out by Spt3 has been conserved from yeast to human.
journal_name
Genomicsjournal_title
Genomicsauthors
Yu J,Madison JM,Mundlos S,Winston F,Olsen BRdoi
10.1006/geno.1998.5500subject
Has Abstractpub_date
1998-10-01 00:00:00pages
90-6issue
1eissn
0888-7543issn
1089-8646pii
S0888-7543(98)95500-8journal_volume
53pub_type
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