Abstract:
:The alpha-fetoprotein (AFP) gene is transcribed at high levels in the fetal liver and is repressed at birth, leading to low but detectable levels of AFP mRNA in the adult liver. This repression is regulated, in part, by a locus that is unlinked to AFP called Alpha-fetoprotein regulator 1 (Afr1). Previous studies showed that Afr1 regulation is independent of the AFP enhancers but requires the 1-kb AFP promoter/repressor region. Here, we demonstrate that a transgene with the 250-bp AFP promoter region linked to AFP enhancer element EII is expressed in the fetal liver and is postnatally repressed. In addition, this transgene is regulated by Afr1. These data indicate that the promoter is involved in postnatal AFP repression. Furthermore, we provide a high-resolution map of the Afr1 locus on mouse chromosome 15.
journal_name
Genomicsjournal_title
Genomicsauthors
Peyton DK,Huang MC,Giglia MA,Hughes NK,Spear BTdoi
10.1006/geno.1999.6073subject
Has Abstractpub_date
2000-01-15 00:00:00pages
173-80issue
2eissn
0888-7543issn
1089-8646pii
S0888-7543(99)96073-1journal_volume
63pub_type
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