Abstract:
:DNA 5' to the human myelin basic protein (MBP) gene, mapped to 18q22----qter, is known to manifest multiallelic DNA length variation with heterozygosity of at least 45%. Isolation of genomic DNA containing the MBP gene first exon and its 5' flanking region reveals that this polymorphism arises from a 994-bp region of the diverged tandem repeat (TGGA)249. This sequence is located from 1082 to 2075 bp upstream of the MBP initiator methionine. The repetitive sequence is 18% diverged from (TGGA)249 and from analysis of higher order subsequence reiterations appears to have undergone extensive recombination. The pattern of higher order repetition suggests that multiple crossover and gene conversion events have occurred within a 1.0-kb region. Molecular clones of this sequence represent essentially the longest allelic form of this region seen in Southern transfer analysis. This repetitive DNA is similar to a sequence 5' to the human myoglobin gene.
journal_name
Genomicsjournal_title
Genomicsauthors
Boylan KB,Ayres TM,Popko B,Takahashi N,Hood LE,Prusiner SBdoi
10.1016/0888-7543(90)90443-xsubject
Has Abstractpub_date
1990-01-01 00:00:00pages
16-22issue
1eissn
0888-7543issn
1089-8646journal_volume
6pub_type
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