Abstract:
:A contig of 20 yeast artificial clones (YACs) has been assembled across 1.5 Mb of Xq28 and formatted with nine previously reported probes and nine STSs developed from the sequence of probes and end fragments of YACs. YAC end fragments were obtained by subcloning, Alu-vector PCR, or primer-ligation PCR methods. Eighteen of the YACs were recovered from a library specific for Xq24-q28; two that fill a gap were obtained from a second library made from total human DNA. One region, containing probes pX78c and 2A1.1, was unstable in YACs, but it was possible to generate a self-consistent map of DNA over the entire contig. Overlaps were confirmed by Southern blot analyses of YAC DNAs, and pulsed-field gel electrophoresis confirmed the extent of the contig and identified at least four CpG islands in the region.
journal_name
Genomicsjournal_title
Genomicsauthors
Palmieri G,Romano G,Casamassimi A,D'Urso M,Little RD,Abidi FE,Schlessinger D,Lagerström M,Malmgren H,Steen-Bondeson MLdoi
10.1006/geno.1993.1234subject
Has Abstract,Author List Incompletepub_date
1993-06-01 00:00:00pages
586-92issue
3eissn
0888-7543issn
1089-8646pii
S0888754383712346journal_volume
16pub_type
杂志文章相关文献
GENOMICS文献大全abstract::Afrotheria genome size is reported to be over 50% larger than that of human, but we show that this is a gross overestimate. Although genome sequencing in Afrotheria is not complete, extensive homology with human has been revealed by chromosome painting. We provide new data on chromosome size and GC content in four Afr...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2013.09.002
更新日期:2013-11-01 00:00:00
abstract::The gene mutated in chorea-acanthocytosis (CHAC; approved gene symbol VPS13A) encodes chorein, a protein similar to yeast Vps13p. We detected several similar putative human proteins by BLAST analysis of chorein. We characterized the structure of three new genes encoding these CHAC-similar proteins, located on chromoso...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2004.04.012
更新日期:2004-09-01 00:00:00
abstract:OBJECTIVE:Cytokines strongly induce expression of the inducible nitric oxide synthase (iNOS) in rodent but not in human endothelial cells. We recently identified NOS2 as a potential target of the histone methyltransferase enhancer of zeste homolog 2 which mediates trimethylation of histone 3 at lysine 27 (H3K27me3). M...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2016.02.002
更新日期:2016-04-01 00:00:00
abstract::Acidic epididymal glycoprotein (AEG), thus far identified only in rodents, is one of the sperm surface proteins involved in the fusion of the sperm and egg plasma membranes. In the present study, we describe the isolation and characterization of cDNA encoding a human glycoprotein related to AEG. Although this protein,...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1996.0131
更新日期:1996-03-15 00:00:00
abstract::We describe the construction and characterization of methylation-resistant sequence-tagged NotI linking clones specific for the X chromosome, referred to as NotI-BsuE linking clones. The approach consists of methylating the X-chromosome-specific cloned DNA with BsuE methylase (M. BsuE), an enzyme that methylates the f...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(91)90108-q
更新日期:1991-09-01 00:00:00
abstract::Biologic effects of androgen on target cells are mediated in part by transcriptional regulation of androgen-regulated genes (ARGs) by androgen receptor. Using serial analysis of gene expression (SAGE), we have identified a comprehensive repertoire of ARGs in LNCaP cells. One of the SAGE-derived tags exhibiting homolog...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.2000.6214
更新日期:2000-06-15 00:00:00
abstract::Chromosome 11p13 is frequently rearranged in individuals with the WAGR syndrome (Wilms tumor, aniridia, genitourinary anomalies, and mental retardation) or parts of this syndrome. To map the cytogenetic aberrations molecularly, we screened DNA from cell lines with known WAGR-related chromosome abnormalities for rearra...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(88)90141-3
更新日期:1988-08-01 00:00:00
abstract::We cloned and characterized a genomic DNA fragment including the deletion junction of a chronic granulomatous disease patient with a 25-kb deletion extending to the 5' two-thirds of CYBB. The 3' breakpoint of the deletion exists in exon 7 of CYBB. A LINE-1 element lies at 5 kb upstream of CYBB in normal persons, and t...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1998.5510
更新日期:1998-10-15 00:00:00
abstract::The deletion of the short arm of chromosome 5 is associated with the cri-du-chat syndrome. In addition, loss of this portion of a chromosome is a common cytogenetic marker in a number of malignancies. However, to date, no genes associated with these disorders have been identified. Physical maps are the first step in i...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1994.1668
更新日期:1994-12-01 00:00:00
abstract::The Long Evans cinnamon (LEC) rat is highly susceptible to X-irradiation due to defective DNA repair and is thus a model for hepatocellular carcinogenesis. We constructed a bacterial artificial chromosome (BAC) contig of rat chromosome 4 completely covering the region associated with radiation susceptibility. We used ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2005.09.020
更新日期:2006-02-01 00:00:00
abstract::We have isolated cDNA and genomic clones coding for the human alpha 7 neuronal nicotinic receptor subunit, the major component of brain nicotinic receptors that are blocked by alpha-bungarotoxin. The human alpha 7 neuronal nicotinic cDNA encodes a mature protein of 479 amino acids that is highly homologous to the rat ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1994.1075
更新日期:1994-01-15 00:00:00
abstract::The gene for prosaposin was characterized by sequence analysis of chromosomal DNA to gain insight into the evolution of this locus that encodes four highly conserved sphingolipid activator proteins or saposins. The 13 exons ranged in size from 57 to 1200 bp, while the introns were from 91 to 3812 bp in length. The reg...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(92)90247-p
更新日期:1992-06-01 00:00:00
abstract::Hemizygous deletions on chromosome 22q11.2 result in developmental disorders referred to as DiGeorge syndrome (DGS)/velocardiofacial syndrome (VCFS). We report the isolation of a novel gene, PCQAP (PC2 glutamine/Q-rich-associated protein), that maps to the DiGeorge typically deleted region and encodes a protein identi...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.2001.6566
更新日期:2001-06-15 00:00:00
abstract::DNA N6-methyladenine (6 mA) is an epigenetic modification that plays a vital role in a variety of cellular processes in both eukaryotes and prokaryotes. Accurate information of 6 mA sites in the Rosaceae genome may assist in understanding genomic 6 mA distributions and various biological functions such as epigenetic i...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2020.09.054
更新日期:2020-10-01 00:00:00
abstract::Background Bioinformatics tools are of great significance and are used in different spheres of life sciences. There are wide variety of tools available to perform primary analysis of DNA and protein but most of them are available on different platforms and many remain undetected. Accessing these tools separately to pe...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2020.08.010
更新日期:2020-11-01 00:00:00
abstract::The ky mouse mutant, kyphoscoliosis, exhibits a degenerative muscle disease resulting in chronic deformation of the spinal column. Using an interspecific backcross segregating the ky mutation, we have mapped the ky locus to a small region of mouse chromosome 9. ky is nonrecombinant with the microsatellites D9Mit24 and...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(95)80127-8
更新日期:1995-01-01 00:00:00
abstract::Multiple inositol polyphosphate phosphatase is the only enzyme known to hydrolyze the abundant metabolites inositol pentakisphosphate and inositol hexakisphosphate. We have previously demonstrated that the chick homolog of multiple inositol polyphosphate phosphatase, designated HiPER1, has a role in growth plate chond...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1998.5736
更新日期:1999-03-15 00:00:00
abstract::Expressed sequence tags (ESTs) have been obtained from several hundred brain cDNAs as an initial effort to characterize expressed brain genes. These ESTs will become tools for human genome mapping and they will also provide candidate causative genes for inherited disorders affecting the central nervous system. We have...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(92)90439-y
更新日期:1992-03-01 00:00:00
abstract::The solute carrier family 22 (SLC22) is a large family of organic cation and anion transporters. These are transmembrane proteins expressed predominantly in kidneys and liver and mediate the uptake and excretion of environmental toxins, endogenous substances, and drugs from the body. Through a comprehensive database s...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2007.03.017
更新日期:2007-11-01 00:00:00
abstract::Human platelet/endothelial cell adhesion molecule-1 (PECAM1), an important member of the immunoglobulin gene superfamily, is widely distributed on cells of the vascular system and mediates cellular interactions through both homophilic and heterophilic adhesive mechanisms. The function of PECAM1 in vitro has begun to b...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1996.0546
更新日期:1996-10-15 00:00:00
abstract::The gene encoding the human type I pituitary adenylate cyclase activating polypeptide receptor (ADCYAP1R1) was mapped to chromosome 7 by PCR analysis of genomic DNA from a human/rodent somatic cell hybrid mapping panel. This assignment was confirmed and the gene localized to chromosome band 7p14 by fluorescence in sit...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1994.1560
更新日期:1994-10-01 00:00:00
abstract::A new Alcanivorax sp. VBW004 was isolated from a shallow hydrothermal vent in Azores Island, Portugal. In this study, we determined VBW004 was resistant to copper. This strain showed maximum tolerance of copper concentrations up to 600 μg/mL. Based on 16S rRNA gene sequencing and phylogeny revealed that this strain wa...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2020.06.015
更新日期:2020-09-01 00:00:00
abstract::The aim of the present study was to identify gene polymorphisms that confer susceptibility to obesity. A total of 5448 unrelated Japanese individuals from two independent populations were examined: subject panel A comprised 4252 individuals who visited participating hospitals; subject panel B comprised 1196 community-...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2008.03.004
更新日期:2008-06-01 00:00:00
abstract::We have analyzed the organization of the chicken alpha-globin gene domain using DNA miniarrays and have found two novel chromatin loop attachment regions. We have found a 40-kb loop domain that includes all the alpha-globin genes in cells of erythroid origin. One of the domain borders colocalizes almost exactly with a...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2004.09.008
更新日期:2005-01-01 00:00:00
abstract::A cDNA clone encoding the major antigen (CDR62) associated with the antibody-induced paraneoplastic cerebellar degeneration has been used to identify the chromosomal location of the corresponding structural gene(s) by screening for its retention in a panel of rodent-human somatic cell hybrids. Having established the s...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(92)90063-x
更新日期:1992-08-01 00:00:00
abstract::In this report we present the genomic, cDNA, and predicted protein sequences for mouse apolipoproteins A-I and CIII, as well as sequence comparisons with other species. The genes for these apolipoproteins are within 2.5 kb of each other and convergently transcribed. The almost 9 kb of genomic sequence presented extend...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/s0888-7543(05)80133-8
更新日期:1992-12-01 00:00:00
abstract::Genes that encode the vertebrate fibrillar collagen types I-III have previously been shown to share a highly conserved intron/exon organization, thought to reflect common ancestry and evolutionary pressures at the protein level. We report here the complete intron/exon organization of COL5A1, the human gene that encode...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1995.9961
更新日期:1995-10-10 00:00:00
abstract::The general strategies of phototransduction in vertebrates and invertebrates share many similarities, but differ significantly in their underlying molecular machinery. The CDS gene encodes the CDP-diacylglycerol synthase (CDS) enzyme and is required for phototransduction in Drosophila. Using a bioinformatic approach, ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1998.5610
更新日期:1999-01-01 00:00:00
abstract::We describe here a 1.2-Mb yeast artificial chromosome (YAC) contig within the region of mouse chromosome 17 between Brachyury (T) and D17Rp17e, and spanning the quaking (qk) region. We describe six new probes distributed across 1.2 Mb: D17Leh502, D17Leh503, D17Leh504, D17Leh505, D17Leh506, and D17Leh507. Probes D17Leh...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1994.1227
更新日期:1994-05-01 00:00:00
abstract::We report the cDNA sequence for the bovine gene for fibrillin corresponding to the human gene, fibrillin 1 (FBN1), and the localization of the gene to bovine chromosome 10 (syntenic group U5). The identity between the human and bovine sequences is 97.8% at the amino acid level and 92% at the nucleotide level. The bovi...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1994.1527
更新日期:1994-09-15 00:00:00