Abstract:
:Activating mutations in the ras genes are commonly found in a wide range of human tumors. We recently cloned two mammalian genes, Son of sevenless 1 (mSos1) and Son of sevenless 2 (mSos2), whose protein products appear to be important positive regulators of ras proteins. Given the proposed role of Sos proteins in ras regulation, and the frequent occurrence of activated ras alleles in tumor cells, we were interested in determining whether the Sos genes may also be activated inappropriately by DNA rearrangement in tumor cells. To investigate this possibility, we have determined the chromosomal locations of both the mouse and the human Sos1 and Sos2 genes, using a combination of genetic linkage analysis and in situ hybridization to chromosomal spreads. We find that the murine Sos1 and Sos2 genes map to chromosomes 17E and 12C3.3-D and their human counterparts to chromosomes 2p21-2p2 and 14q21, respectively. Neither the human nor the mouse Sos loci map close to known mutations or to regions showing consistent karyotypic abnormalities in tumor cells.
journal_name
Genomicsjournal_title
Genomicsauthors
Webb GC,Jenkins NA,Largaespada DA,Copeland NG,Fernandez CS,Bowtell DDdoi
10.1006/geno.1993.1421subject
Has Abstractpub_date
1993-10-01 00:00:00pages
14-9issue
1eissn
0888-7543issn
1089-8646pii
S0888-7543(83)71421-7journal_volume
18pub_type
杂志文章相关文献
GENOMICS文献大全abstract::We have established a method for amplifying and obtaining large quantities of chromosome-specific DNA by linker/adaptor ligation and polymerase chain reaction (PCR). Small quantities of DNA isolated from flow cytometry-sorted chromosomes 17 and 21 were digested with MboI, ligated to a linker/adaptor, and then subjecte...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(92)90378-6
更新日期:1992-02-01 00:00:00
abstract::Several projects have produced maps of the physical position of genes within the human genome, either on a genome-wide scale or of a more detailed subsection of a chromosome. However, these maps largely rely on the mapping of expressed sequences (cDNAs and ESTs) back onto physical maps by their localization onto speci...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1999.6075
更新日期:2000-02-01 00:00:00
abstract::We describe the construction and characterization of methylation-resistant sequence-tagged NotI linking clones specific for the X chromosome, referred to as NotI-BsuE linking clones. The approach consists of methylating the X-chromosome-specific cloned DNA with BsuE methylase (M. BsuE), an enzyme that methylates the f...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(91)90108-q
更新日期:1991-09-01 00:00:00
abstract::Comparative genetic analysis between human and chimpanzee may detect genetic divergences responsible for human-specific characteristics. Previous studies have identified a series of genes that potentially underwent Darwinian positive selection during human evolution. However, without a closely related species as outgr...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2006.05.008
更新日期:2006-12-01 00:00:00
abstract::The Tcp-1 gene is located in the t-complex region of mouse chromosome 17 and on the long arm of human chromosome 6. In the mouse, a related gene, Tcp-1x, is tightly linked to Tcp-1. It is shown here that two genes located 3' to the murine Tcp-1 and Tcp-1x genes code for proteins highly homologous to acetyl-CoA acetylt...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1993.1454
更新日期:1993-11-01 00:00:00
abstract::We have isolated cDNA and genomic clones coding for the human alpha 7 neuronal nicotinic receptor subunit, the major component of brain nicotinic receptors that are blocked by alpha-bungarotoxin. The human alpha 7 neuronal nicotinic cDNA encodes a mature protein of 479 amino acids that is highly homologous to the rat ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1994.1075
更新日期:1994-01-15 00:00:00
abstract::Using the human/hamster cell line UCTP2A-3, we have generated and isolated three hybrids, each containing a novel human chromosome 3p break. All chromosome 3 materials distal to the breaks were lost. Two of the breakpoints were produced using aphidicolin induction; the third breakpoint occurred spontaneously. The aphi...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/s0888-7543(05)80110-7
更新日期:1992-12-01 00:00:00
abstract::Human chromosome 11 harbors many genes of medical significance and cancer-related rearrangements. The availability of cloned DNA in cosmids and in yeast artificial chromosomes (YACs), combined with fluorescence in situ hybridization analysis, has led to the cloning of genes at sites of chromosomal breakpoints in acute...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1994.1354
更新日期:1994-07-01 00:00:00
abstract::Differentiation of Wharton's Jelly-Mesenchymal Stem cells (WJ-MSCs) into cardiomyocytes (CMs) in vitro has been reported widely although contradictions remain regarding the maturation of differentiated MSCs into fully functioning CMs. Studies suggest that use of epigenetic modifiers like 5'Azacytidine (5-AC) in MSCs d...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2019.08.007
更新日期:2020-03-01 00:00:00
abstract::One of the most critical periods of embryonic loss in pig is day 12 of pregnancy, when implantation begins. Here, we analyzed the gene expression on day 12 of pregnancy and non-pregnancy in the porcine endometrium using RNA sequencing (RNA-seq). 237 mRNAs, 34 lncRNAs and 1 miRNA were significantly differentially expre...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2016.11.007
更新日期:2017-01-01 00:00:00
abstract::The primary structure of the human microsomal glutathione S-transferase gene (GST12) was determined by genomic cloning. The gene structure of GST12 spans 12.8 kb and consists of four exons and three introns. The coding sequence resides on exons 2, 3, and 4. Sequencing of the exons revealed two nucleotide differences c...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1996.0429
更新日期:1996-08-15 00:00:00
abstract::A complete genetic linkage map of the soybean, in which sequence-based (SB) genetic markers are evenly distributed genomewide, was constructed from an F(12) population composed of 113 recombinant inbred lines derived from an interspecific cross involving Korean genotypes Hwangkeum and IT182932. Several approaches were...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2008.03.008
更新日期:2008-07-01 00:00:00
abstract::Abnormal histone modifications (HMs) and transcription factors (TFs) can alter the expression of cancer-related genes to promote tumorigenesis. We studied the variations of 11 HMs and 2 TFs in human breast cancer cells (MCF-7) compared to human normal mammary epithelial cells (HMEC), and the effects of HMs/TFs in vari...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2019.05.026
更新日期:2020-01-01 00:00:00
abstract::Fruit development and ripening are essential components of human and animal diets. Fruit ripening is also a vital plant trait for plant shelf life at the commercial level. In the present study, two apple cultivars, Hanfu wild (HC) and Hanfu mutant (HM), were employed for RNA-Sequencing (RNA-Seq) to explore the genes i...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2020.09.040
更新日期:2020-09-20 00:00:00
abstract::Desmoglein is a transmembrane glycoprotein of the cadherin superfamily present in the desmosomal junction in vertebrate epithelial cells. At least two variants of desmoglein are differentially expressed in human tissues: DGI, a characteristic desmosomal protein; and HDGC, which is, for example, expressed in the simple...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(92)90280-6
更新日期:1992-06-01 00:00:00
abstract::Tandem repetitive sequences are DNA motifs common in the genomes of eukaryotic species and are often embedded in heterochromatic regions. In most eukaryotes, ribosomal genes, as well as centromeres and telomeres or subtelomeres, are associated with abundant tandem arrays of repetitive sequences and typically represent...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2008.04.008
更新日期:2008-08-01 00:00:00
abstract::The domestic dog is increasingly being recognized as a useful model for human disease. The aim of this study was to conduct the first detailed whole-genome comparison of human and dog using bidirectional heterologous chromosome painting (reciprocal Zoo-FISH) analysis. We used whole-chromosome paint probes produced fro...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1999.5947
更新日期:1999-10-15 00:00:00
abstract::Cloning and characterization of the cystic fibrosis transmembrane conductance regulator (CFTR) gene led to the identification and isolation of cDNA and genomic sequences that cross-hybridized to the first nucleotide binding fold of CFTR. DNA sequence analysis of these clones showed that the cross-hybridizing sequences...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1997.4968
更新日期:1997-11-01 00:00:00
abstract::The large number of redundant sequences available in nucleotide databases provides a resource for the identification of polymorphisms. Expressed polymorphisms in X-linked genes can be used to determine the inactivation status of the genes, and polymorphisms in genes that are subject to inactivation can then be used as...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.2000.6153
更新日期:2000-04-01 00:00:00
abstract::Ten microsatellite DNA polymorphisms located on human chromosome 10 were regionally mapped using subchromosomal somatic cell hybrids and linkage analysis. The resulting order of the markers from pter-qter was [D10S89, D10S111], D10S107, D10S109, [D10S91, D10S110, D10S108, D10S88, D10S168], and D10S169. Order of the ma...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(92)90455-2
更新日期:1992-03-01 00:00:00
abstract::Indolethylamine N-methyltransferase (INMT) catalyzes the N-methylation of tryptamine and structurally related compounds. We recently cloned and characterized the rabbit INMT cDNA and gene as a step toward cloning the cDNA and gene for this enzyme in humans. We have now used a PCR-based approach to clone a human INMT c...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1999.5960
更新日期:1999-11-01 00:00:00
abstract::Next generation sequencing techniques produce enormous data but its analysis and visualization remains a big challenge. To address this, we have developed Genome Annotator Light(GAL), a Docker based package for genome analysis and data visualization. GAL integrated several existing tools and in-house programs inside a...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2019.03.012
更新日期:2020-01-01 00:00:00
abstract::In extracellular fluids the insulin-like growth factors (IGFs) are bound to specific binding proteins (IGBPs). The genes for two members of this protein family have been mapped, the IGBP1 gene to human chromosomal region 7p14-p12 and the IGBP2 gene to region 2q33-q34. In this study, somatic cell hybrid analysis indica...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(92)90440-4
更新日期:1992-03-01 00:00:00
abstract::Inducible costimulatory receptor (ICOS) is one recently identified member of the CD28 family of costimulatory molecules. Evidence suggests ICOS functions as a critical immune regulator and, to evaluate these effects, we employed the canine model system that has been used to develop strategies currently in clinical use...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2004.06.009
更新日期:2004-10-01 00:00:00
abstract::The gene encoding the brain alpha 2-adrenergic receptor (ADRA2C) is located on human chromosome 4. It has been circumstantially associated with a number of human disorders, including Parkinson disease, panic disorders, and Huntington disease (HD). Using somatic cell hybrids, we localized the gene to chromosome 4p16 di...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1994.1061
更新日期:1994-01-15 00:00:00
abstract::FISH techniques have opened new possibilities for high-resolution genome mapping. Effective utilization of these techniques for the rapid orientation and ordering of adjacent and overlapping probes as well as for the characterization of long-range genomic contigs would facilitate physical mapping and positional clonin...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1995.0005
更新日期:1995-11-01 00:00:00
abstract::The proximal end of mouse chromosome (Chr) 13 contains regions conserved on human chromosomes 1q42-q44, 6p23-p21, and 7p22-p13. This region also contains mutations that may be models for human disease, including beige (human Chediak-Higashi syndrome). An interspecific backcross of SB/Le and Mus spretus mice was used t...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1996.4478
更新日期:1997-01-15 00:00:00
abstract::Developmental dysplasia of the hip (DDH) is a common congenital malformation characterized by mismatch in shape between the femoral head and acetabulum, and leads to hip dysplasia. To date, the pathogenesis of DDH is poorly understood and may involve multiple factors, including genetic predisposition. However, compreh...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2018.02.006
更新日期:2019-05-01 00:00:00
abstract::Thirty-five new, unique, DNA probes have been isolated and each has been assigned to one of five regions on chromosome 22. The distribution of probes along the chromosome is what would be expected based on the estimated size of each region with the exception of the short arm (22p). RFLP analysis was performed using 13...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(91)90190-p
更新日期:1991-08-01 00:00:00
abstract::Eight genes located on the short arm of the human X chromosome (MAOA, SYN1, OAT, OTC, CYBB, DMD, ZFX, POLA) have been mapped in several marsupial species by cell hybrid analysis and/or in situ hybridization using probes derived from human cDNA. Seven appear to be autosomal in all marsupial species examined. The eighth...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(91)90141-z
更新日期:1991-10-01 00:00:00