Abstract:
:We have cloned a basic helix-loop-helix (bHLH) factor gene, Bhlhb4, from a mouse beta-cell line. Fluorescence in situ hybridization (FISH) and genetic mapping place Bhlhb4 at the telomeric end of mouse chromosome 2 (H3-H4), syntenic to human chromosome 20q13. Based on phylogenetic analysis, BHLHB4 belongs to a new subgroup of bHLH factors including at least four previously identified mouse bHLH factors: BHLHB5, MIST1, OLIG1, OLIG2, and OLIG3. In the developing nervous system, Bhlhb4 was found to mark the dimesencephalic boundary, suggesting that Bhlhb4 may have a role in diencephalic regionalization. In the pancreas, Bhlhb4 is expressed in a transient fashion that suggests a role in the pancreatic endocrine cell lineage. Transfection experiments show that BHLHB4 can repress transcriptional activation mediated through the pancreatic beta-cell specific insulin promoter enhancer RIPE3. Together, these data suggest that BHLHB4 may modulate the expression of genes required for the differentiation and/or maintenance of pancreatic and neuronal cell types.
journal_name
Genomicsjournal_title
Genomicsauthors
Bramblett DE,Copeland NG,Jenkins NA,Tsai MJdoi
10.1006/geno.2002.6708subject
Has Abstractpub_date
2002-03-01 00:00:00pages
402-12issue
3eissn
0888-7543issn
1089-8646pii
S088875430296708Xjournal_volume
79pub_type
杂志文章相关文献
GENOMICS文献大全abstract::Using a panel of patient cell lines with chromosomal breakpoints, we constructed a physical map for the short arm of human chromosome 11. We focused on 11p15, a chromosome band harboring at least 25 known genes and associated with the Beckwith-Wiedemann syndrome, several childhood tumors, and genomic imprinting. This ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1994.1312
更新日期:1994-06-01 00:00:00
abstract::The desmogleins, together with the desmocollins, both members of the cadherin superfamily, are the adhesive proteins of the desmosome type of cell junction, characteristically found in epithelial cells. Three different human desmoglein isoforms are encoded by separate genes (DSG1, DSG2, and DSG3) located on chromosome...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(95)80067-v
更新日期:1995-01-20 00:00:00
abstract::To refine the linkage map of mouse Chromosome (Chr) 12 and to define better the homology relationships between it and human chrs 2p and 14q, nine new anonymous DNA markers of Chr 12 were identified, and mouse loci homologous to the human D14S17, CHGA, HSPA2, RRM2, TPO, and ZFP50 ("KUP") genes were defined. The inherit...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1994.1234
更新日期:1994-05-01 00:00:00
abstract::Hermansky-Pudlak syndrome (HPS) is a group of human disorders of organelle biogenesis characterized by defective synthesis of melanosomes, lysosomes, and platelet dense granules. In the mouse, at least 15 loci are associated with mutant phenotypes similar to human HPS. We have identified the gene mutated in cocoa (coa...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.2001.6644
更新日期:2001-11-01 00:00:00
abstract::TR4 is a new member of the nuclear hormone receptor family. This receptor is highly conserved in rat and human, but an in-frame insertion of 19 amino acid residues in the amino-terminal (A/B) region was found in the human homolog, which we refer to as hTR4alpha1. By reverse transcription-PCR (RT-PCR) we have identifie...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1996.0368
更新日期:1996-07-15 00:00:00
abstract::The major histocompatibility complex (MHC) class I region has been shown to be associated with a variety of immune and nonimmune disorders. In an effort to initiate steps designed to identify the idiopathic hemochromatosis disease gene (HFE), we have cloned and mapped two expressed messages using probes from the HLA-H...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1994.1382
更新日期:1994-07-15 00:00:00
abstract::Long sequencing reads offer unprecedented opportunities in analysis and reconstruction of complex genomic regions. However, the gain in sequence length is often traded for quality. Therefore, recently several approaches have been proposed (e.g. higher sequencing coverage, hybrid assembly or sequence correction) to enh...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2017.12.011
更新日期:2019-01-01 00:00:00
abstract::The inheritance of obesity has been analyzed in an intercross between the lean 129/Sv mouse strain and the obesity-prone EL/Suz mouse strain. The weights of three major fat pads were determined on 4-month-old mice, and the sum of these weights, divided by body weight, was used as an adiposity index. The strategy of se...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1996.0302
更新日期:1996-06-15 00:00:00
abstract::lncRNAs are a class of transcriptional RNA molecules of >200 nucleotides in length. However, the overall expression pattern and function of lncRNAs in sheep muscle is not clear. Here, we identified 1566 lncRNAs and 404 differentially expressed lncRNAs in sheep muscle from prenatal (110 days of fetus) and postnatal (2 ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2018.01.009
更新日期:2019-03-01 00:00:00
abstract::Cell wall lytic enzymes play key roles in biochemical, morphological, genetic research and industry fields. To save time and labor costs, bioinformatic methods are usually adopted to narrow the scope of in vitro experimentation. In this paper, we established a novel machine learning (support vector machine) based iden...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2020.08.015
更新日期:2020-11-01 00:00:00
abstract::A method was recently developed for the specific amplification of human DNA sequences from interspecific somatic cell hybrids by the polymerase chain reaction (PCR) using primers directed to Alu, a short interspersed repeat element (SINE). We now show human-specific amplification using a primer to the 3' end of the hu...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(90)90477-c
更新日期:1990-03-01 00:00:00
abstract::The Alu repeat sequence is estimated to account for 5% of human genomic DNA. The precise relationship of Alu sequences to human fully spliced cDNA has yet to be determined, although many new protocols for cloning cDNAs either depend on the presence of Alus or--more usually--rely on their absence in a population of mes...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1995.1090
更新日期:1995-06-10 00:00:00
abstract::Low-molecular-weight, calcium-dependent phospholipase A2 genes (PLA2s) that belong to the secreted type of PLA2s are clustered within a syntenic group on human 1p35-p36 and mouse 4qD3. We reassembled trace files available from the Whole Genome Sequencing (WGS) Project, obtaining an 86-kb contig with three tandem PLA2G...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2005.12.015
更新日期:2006-04-01 00:00:00
abstract::In this study, we systematically screened the polymorphisms of the whole CYP2D6 gene in the populations of four different geographical locations in China, namely, Shanghai, Shantou, Shenyang, and Xi'an, using a sample of 100 subjects from each population. Forty-eight different polymorphisms were detected as well as 12...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2008.05.004
更新日期:2008-09-01 00:00:00
abstract::The complexity of the somatic embryogenesis (SE) transcriptome suggests that numerous molecules are involved. To understand better the functional genomics of complex molecular systems during this important reprogramming process, we used bioinformatics and a pathway database to construct a draft network based on transc...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2007.07.007
更新日期:2007-11-01 00:00:00
abstract::A mouse inositol polyphosphate 1-phosphatase (Inpp1) cDNA fragment (348 bp) was amplified by means of the polymerase chain reaction using a mouse cDNA library as template with primers designed from published human and bovine cDNA sequences. We isolated a 1623-bp full-length Inpp1 cDNA from a mouse brain cDNA library u...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1995.0030
更新日期:1995-11-20 00:00:00
abstract::Polymorphic loci that lie at the two extremities of the Duchenne/Becker muscular dystrophy (DMD/BMD) gene have been used to estimate intragenic recombination rates. Multipoint linkage analysis of the CEPH panel of families suggests a total intragenic recombination frequency of nearly 0.12 (confidence intervals 0.041-0...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(90)90205-9
更新日期:1990-08-01 00:00:00
abstract::We have used radiation hybrid (RH) mapping and pulsed-field gel electrophoresis (PFGE) to determine the order and positions of 28 DNA markers from the distal region of the long arm of human chromosome 21. The maps generated by these two methods are in good agreement. This study, combined with that of D. R. Cox et al. ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(91)90216-2
更新日期:1991-01-01 00:00:00
abstract::Intercellular adhesion molecule-1 (ICAM-1) is an integral membrane protein, a member of the immunoglobulin superfamily, and a ligand for LFA-1, a beta 2 leukocyte integrin. ICAM-1 has a tissue distribution similar to that of the major histocompatibility complex class II antigens and is likely to play a role in inflamm...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(91)90423-c
更新日期:1991-03-01 00:00:00
abstract::The chromosomal band 17p11.2 is associated with a number of neurological disorders and malignant diseases. This region is also characterized by the presence of complex repeat elements that are probably responsible for the frequent occurrence of interstitial deletions, duplications, and isochromosome formation. In the ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1998.5647
更新日期:1999-02-15 00:00:00
abstract::Myeloproliferative disorders frequently show deletions or rearrangements of the long arm of chromosome 13. We report here the cloning of RNF6, a new gene that maps close to the chromosome 13 breakpoint in a case of myelofibrosis with a t(4;13)(q26;q12). RNF6 is predicted to encode a 685-amino-acid protein with a coile...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1999.5802
更新日期:1999-05-15 00:00:00
abstract::In this investigation, whole-genome identification and functional characterization of the cotton dehydrin genes was carried out. A total of 16, 7, and 7 dehydrin proteins were identified in G. hirsutum, G. arboreum and G. raimondii, respectively. Through RNA sequence data and RT-qPCR validation, Gh_A05G1554 (GhDHN_03)...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2019.11.003
更新日期:2020-03-01 00:00:00
abstract::Using library to library cross-screening we have identified a number of genomic clones that harbor X-linked sequences expressed in the human choroid/retina. We describe the characterization of one of these, designated XEH.8 (DXS542), which is localized to Xp11.3-q12. Isolation, partial sequencing, and Northern analysi...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1993.1096
更新日期:1993-03-01 00:00:00
abstract::This study has investigated the transcriptional regulation of the Emr1 gene in murine macrophages and defined an enhancer element within the proximal promoter that is necessary for Emr1 expression in myeloid cells. This element consists of an extended purine-rich sequence (PuRS) of 83 consecutive purine residues conta...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2004.08.016
更新日期:2004-12-01 00:00:00
abstract::The genome sequence of Mycobacterium tuberculosis strain H37Rv is an important and valuable reference point in the study of M. tuberculosis phylogeny, molecular epidemiology, and drug-resistance mutations. However, it is becoming apparent that use of H37Rv as a sole reference genome in analysing clinical isolates pres...
journal_title:Genomics
pub_type: 杂志文章,评审
doi:10.1016/j.ygeno.2017.07.004
更新日期:2017-10-01 00:00:00
abstract::We have mapped the mouse protein tyrosine phosphatase epsilon (PTP epsilon, gene symbol Ptpre) gene to the distal region of chromosome 7 by linkage analysis using two sets of multilocus genetic crosses. The human PTP epsilon gene (gene symbol PTPRE) was mapped to chromosome 10q26 by fluorescence in situ hybridization....
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1996.0061
更新日期:1996-02-01 00:00:00
abstract::A cDNA clone encoding the major antigen (CDR62) associated with the antibody-induced paraneoplastic cerebellar degeneration has been used to identify the chromosomal location of the corresponding structural gene(s) by screening for its retention in a panel of rodent-human somatic cell hybrids. Having established the s...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(92)90063-x
更新日期:1992-08-01 00:00:00
abstract::Despite antibiotic therapy and vaccination programs, microbial diseases continue to be the leading cause of morbidity and mortality worldwide. The genetic basis of the host response to infection is complex, and its understanding has been facilitated through the study of mouse models of human infectious diseases. Genet...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1997.5116
更新日期:1998-01-15 00:00:00
abstract::RIMs are synaptic proteins that are essential for normal neurotransmitter release. We now show that while invertebrates contain only a single RIM gene, vertebrates contain four: two large genes encoding RIM1alpha (0.50 Mb) or RIM2alpha, 2beta, and 2gamma (0.50-0.75 Mb) and two smaller genes encoding RIM3gamma (14 kb) ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/s0888-7543(02)00024-1
更新日期:2003-02-01 00:00:00
abstract::Understanding differences in the repertoire of orthologous gene pairs is vital for interpretation of pharmacological and physiological experiments if conclusions are conveyed between species. Here we present a comprehensive dataset for G protein-coupled receptors (GPCRs) in both human and mouse with a phylogenetic roa...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2006.04.001
更新日期:2006-09-01 00:00:00