Abstract:
:In this investigation, whole-genome identification and functional characterization of the cotton dehydrin genes was carried out. A total of 16, 7, and 7 dehydrin proteins were identified in G. hirsutum, G. arboreum and G. raimondii, respectively. Through RNA sequence data and RT-qPCR validation, Gh_A05G1554 (GhDHN_03) and Gh_D05G1729 (GhDHN_04) were highly upregulated, and knockdown of the two genes, significantly reduced the ability of the plants to tolerate the effects of osmotic and salt stress. The VIGS-plants recorded significantly higher concentration levels of oxidants, hydrogen peroxide (H2O2) and malondialdehyde (MDA), furthermore, the four stress responsive genes GhLEA2, Gh_D12G2017 (CDKF4), Gh_A07G0747 (GPCR) and a transcription factor, trihelix, Gh_A05G2067, were significantly downregulated in VIGS-plants, but upregulated in wild types under osmotic and salt stress condition. The result indicated that dehydrin proteins are vital for plants and can be exploited in developing a more osmotic and salt stress-resilient germplasm to boost and improve cotton production.
journal_name
Genomicsjournal_title
Genomicsauthors
Kirungu JN,Magwanga RO,Pu L,Cai X,Xu Y,Hou Y,Zhou Y,Cai Y,Hao F,Zhou Z,Wang K,Liu Fdoi
10.1016/j.ygeno.2019.11.003subject
Has Abstractpub_date
2020-03-01 00:00:00pages
1902-1915issue
2eissn
0888-7543issn
1089-8646pii
S0888-7543(19)30659-7journal_volume
112pub_type
杂志文章相关文献
GENOMICS文献大全abstract::A number of murine cataract mutations have been localized to chromosome 1 close to the gamma-crystallin gene cluster (Cryg) (Everett et al., 1994, Genomics 20: 429-434; Löster et al., 1994, Genomics 23: 240-242). Based on the size of the mapping or allelism tests they have not been shown to be genetically distinct and...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1998.5417
更新日期:1998-09-01 00:00:00
abstract::FISH techniques have opened new possibilities for high-resolution genome mapping. Effective utilization of these techniques for the rapid orientation and ordering of adjacent and overlapping probes as well as for the characterization of long-range genomic contigs would facilitate physical mapping and positional clonin...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1995.0005
更新日期:1995-11-01 00:00:00
abstract::Laboratory mouse strains are known to have emerged from recent interbreeding between individuals of Mus musculus isolated populations. As a result of this breeding history, the collection of polymorphisms observed between laboratory mouse strains is likely to harbor the effects of natural selection between reproductiv...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2010.02.004
更新日期:2010-04-01 00:00:00
abstract::The recent identification of an intragenic differentially methylated region (DMR) within the last exon of the bovine Insulin-like growth factor 2 (IGF2) gene provides a diagnostic tool for in-depth investigation of bovine imprinting and regulatory mechanisms which are active during embryo development. Here, we used bi...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2009.03.004
更新日期:2009-07-01 00:00:00
abstract::Environmental decline triggers a switch in reproductive strategy of Daphnia magna from asexual to sexual reproduction; however, the molecular basis of such environmental sex determination remains largely unknown. In insects most closely related to branchiopod crustaceans, orthologs of the Drosophila transformer gene s...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2009.12.005
更新日期:2010-03-01 00:00:00
abstract::The human homeobox gene HLX1 appears to be involved in hemopoietic development and may represent a candidate gene for various developmental or hemopoietic disorders. We have isolated genomic clones for the gene, determined its intron-exon organization, and confirmed its map location on chromosome 1q41-q42. The transcr...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1994.1394
更新日期:1994-07-15 00:00:00
abstract::CircRNA is a specific type of non-coding RNA that has been shown to have an important role in mammary gland (MG) activity, but no study of MG circRNA activity in sheep so far. In this study, the expression profile of circRNAs was investigated using RNA-Seq in MG parenchyma at peak lactation from Small-Tailed Han sheep...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2019.12.014
更新日期:2020-05-01 00:00:00
abstract::The myotonic dystrophy (DM) region has been recently shown to be bracketed by two key recombinant events. One recombinant occurs in a Dutch DM family, which maps the DM locus distal to the ERCC1 gene and D19S115 (pE0.8). The other recombinant event is in a French Canadian DM family, which maps DM proximal to D19S51 (p...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(92)90119-d
更新日期:1992-07-01 00:00:00
abstract::The retina and its adjacent supporting tissues - retinal pigmented epithelium (RPE) and choroid - are critical structures in human eyes required for normal visual perception. Abnormal changes in these layers have been implicated in diseases such as age-related macular degeneration and glaucoma. With the advent of high...
journal_title:Genomics
pub_type: 杂志文章,评审
doi:10.1016/j.ygeno.2015.01.008
更新日期:2015-05-01 00:00:00
abstract::An exon representing a novel clathrin heavy chain gene (CLTCL) was isolated during gene identification studies and transcription mapping of human chromosome 22. Isolation and sequencing of cDNA clones corresponding to this exon revealed extensive similarity of the predicted amino acid sequence of this gene product to ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1996.0386
更新日期:1996-08-01 00:00:00
abstract::We have isolated and sequenced part of a new gene of the tyrosine kinase family. This gene, called FLT3, has strong sequence similarities with members of a group of genes encoding growth factor receptors: FMS, KIT, and PDGFR. We have localized the human FLT3 gene to chromosome 13, band q12, and its mouse homolog to ch...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(91)90270-o
更新日期:1991-02-01 00:00:00
abstract::We describe here a method for DNA fingerprinting of human chromosomes by Alu-polymerase chain reaction (PCR) amplification of DNA from monochromosomal hybrids, following digestion with restriction endonucleases. DNA digestion with restriction enzymes prior to PCR amplification reduces the total number of amplified fra...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/s0888-7543(05)80175-2
更新日期:1992-11-01 00:00:00
abstract::Using antibodies directed against the amino-terminus of dystrophin, we identified a truncated protein in a Duchenne muscular dystrophy patient. Antibodies directed against the carboxy-terminus failed to identify any cross-reactive material, a result consistent with premature termination of dystrophin translation. The ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(91)90332-9
更新日期:1991-06-01 00:00:00
abstract::Single Nucleotide Polymorphism (SNP) is one of the important molecular markers widely used in animal breeding program for improvement of any desirable genetic traits. Considering this, the present study was carried out to identify, annotate and analyze the SNPs related to four important traits of buffalo viz. milk vol...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2020.04.012
更新日期:2020-09-01 00:00:00
abstract::Cytogenetic studies of banded chromosomes and fluorescence in situ hybridization (FISH) of several yeast artificial chromosomes (YACs) that are part of a 128-kb resolution physical map of a portion of 12q13 revealed that 4/14 (28%) lipomas have breakpoints in 12q13. These breakpoints are more than 10 Mb away from the ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1997.4993
更新日期:1997-11-15 00:00:00
abstract::The genes for three subtilisin/kexin-like proprotein convertases, PC4, PC5, and PACE4, were mapped in the mouse by RFLP analysis of a DNA panel from a (C57BL/6JEi x SPRET/Ei)F1 x SPRET/Ei backcross. The chromosomal locations of the human homologs were determined by Southern blot analysis of a DNA panel from human-rode...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(95)80090-9
更新日期:1995-03-01 00:00:00
abstract::We have constructed a long-range contig of cosmid and YAC clones around D10S102, a locus that is tightly linked to the gene responsible for multiple endocrine neoplasia type 2A (MEN2A). With D10S102 as a starting point, a 360-kb cosmid contig was constructed by bidirectional genomic walking, and at least six fragments...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(92)90390-e
更新日期:1992-02-01 00:00:00
abstract::SHIP2 is a new member of the inositol polyphosphate 5-phosphatase family showing homology to SHIP1. The structure of both enzymes is characterized by the presence of a 5' SH2 domain, a central catalytic domain, and a 3' proline-rich region. Recent results suggest that SHIP2 and SHIP1 act downstream of various receptor...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1999.5995
更新日期:1999-12-01 00:00:00
abstract::Thirty-five new, unique, DNA probes have been isolated and each has been assigned to one of five regions on chromosome 22. The distribution of probes along the chromosome is what would be expected based on the estimated size of each region with the exception of the short arm (22p). RFLP analysis was performed using 13...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(91)90190-p
更新日期:1991-08-01 00:00:00
abstract::Neurotensin (NTS) is an endogenous tridecapeptide of the central nervous system and the gastrointestinal tract of different mammalian species including human. The human gene encoding neurotensin has previously been assigned to chromosome 12 but no regional localization was available. We now confirm this assignment and...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1996.0624
更新日期:1996-12-01 00:00:00
abstract::Chromosomal locations have been assigned to seven members of the TGF-beta superfamily using an interspecific mouse backcross. Probes for the Tgfb-1, -2, and -3, Bmp-2a and -3, and Vgr-1 genes recognized only single loci, whereas the Bmp-2b probe recognized two independently segregating loci (designated Bmp-2b1 and Bmp...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(90)90480-i
更新日期:1990-03-01 00:00:00
abstract::Amyloid beta protein (beta A4), the major component of the core of amyloid plaques in Alzheimer disease, is derived from the transmembrane amyloid precursor proteins (APPs). Our recent studies showed that a murine member of the evolutionarily conserved APP family, amyloid precursor-like protein 1 (APLP1), is specifica...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1996.0096
更新日期:1996-02-15 00:00:00
abstract:INTRODUCTION:Long non-coding RNAs (lncRNAs) have fundamental roles in cell migration, proliferation, invasion and metastasis. METHODS:In the current study, we evaluated expression of a panel of lncRNAs in bladder cancer tissues, adjacent non-cancerous tissues (ANCTs) and normal bladder tissues to evaluate their diagno...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2019.04.020
更新日期:2020-01-01 00:00:00
abstract::Aldehyde reductase (EC 1.1.1.2; AKR1A1) is involved in the reduction of biogenic and xenobiotic aldehydes and is present in virtually every tissue. To study the regulation of its expression, the human aldehyde reductase gene and promoter were cloned and characterized. The protein coding region consists of eight exons,...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1999.5915
更新日期:1999-09-01 00:00:00
abstract::Amaranth has been proposed as an exceptional alternative for food security and climate change mitigation. Information about the distribution, abundance, or specificity of miRNAs in amaranth species is scare. Here, small RNAs from seedlings under control, drought, heat, and cold stress conditions of the Amaranthus hypo...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2020.11.027
更新日期:2021-01-01 00:00:00
abstract::Knowledge of protein subcellular localization is vitally important for both basic research and drug development. With the avalanche of protein sequences emerging in the post-genomic age, it is highly desired to develop computational tools for timely and effectively identifying their subcellular localization purely bas...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2018.05.017
更新日期:2019-07-01 00:00:00
abstract::Neogenin was first identified in the chick embryo, and like a number of cell surface proteins of the immunoglobulin (Ig) superfamily, including N-CAM and L1 (generally called cell adhesion molecules or CAMs), it is expressed on growing nerve cells in the developing nervous system of vertebrate embryos. Neogenin is als...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1997.4688
更新日期:1997-05-01 00:00:00
abstract::Mapping of 33 anonymous DNA probes and 12 genes to the long arm of chromosome 16 was achieved by the use of 14 mouse/human hybrid cell lines and the fragile site FRA16B. Two of the hybrid cell lines contained overlapping interstitial deletions in bands q21 and q22.1. The localization of the 12 genes has been refined. ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(91)90313-4
更新日期:1991-06-01 00:00:00
abstract::The AZ1 protein is localized to the preacrosome region of spermatids. Previous developmental studies suggested that AZ1 gene transcription begins in pachytene spermatocytes and that expression of the gene is induced in cultivated fibroblasts on treatment with 5-azacytidine, which is known to lead to the demethylation ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1996.4546
更新日期:1997-02-15 00:00:00
abstract::Interleukin-1 beta (IL-1 beta) mediates a wide range of immune and inflammatory responses. The active cytokine is generated by proteolytic cleavage of an inactive precursor by a protease called the IL-1 beta converting enzyme (ICE). A cDNA encoding this protease was recently isolated. A human genomic clone containing ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1994.1202
更新日期:1994-04-01 00:00:00