Abstract:
:We have constructed a long-range contig of cosmid and YAC clones around D10S102, a locus that is tightly linked to the gene responsible for multiple endocrine neoplasia type 2A (MEN2A). With D10S102 as a starting point, a 360-kb cosmid contig was constructed by bidirectional genomic walking, and at least six fragments from these cosmids showed high sequence homology to other species. Five YAC clones were also isolated at the D10S102 locus, and they formed a contig covering 950 kb of genomic DNA. Furthermore, we obtained six RFLP systems from the contig, which will serve as new resources for fine-scale genetic linkage mapping of the MEN2A locus.
journal_name
Genomicsjournal_title
Genomicsauthors
Tokino T,Imai T,Tanigami A,Takiguchi S,Nakamura Ydoi
10.1016/0888-7543(92)90390-esubject
Has Abstractpub_date
1992-02-01 00:00:00pages
394-400issue
2eissn
0888-7543issn
1089-8646pii
0888-7543(92)90390-Ejournal_volume
12pub_type
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