Abstract:
:Neogenin was first identified in the chick embryo, and like a number of cell surface proteins of the immunoglobulin (Ig) superfamily, including N-CAM and L1 (generally called cell adhesion molecules or CAMs), it is expressed on growing nerve cells in the developing nervous system of vertebrate embryos. Neogenin is also expressed in other embryonic tissues, suggesting a more general role in developmental processes such as tissue growth regulation, cell-cell recognition, and cell migration. Neogenin, unlike the CAMs, is closely related to a unique tumor suppressor candidate molecule, deleted in colorectal carcinoma (DCC). Like DCC, the neogenin protein consists of four immunoglobulin-like (Ig-like) domains followed by six fibronectin type III domains, a transmembrane domain, and an intracellular domain. We now report the cloning and sequencing of cDNA clones coding for the human neogenin protein. Human neogenin shares 87% identity with its chicken homolog, and like its chicken counterpart it is expressed in at least two different isoforms derived from alternative splicing in the intracellular domain. Northern blot analysis revealed two mRNA species of about 5 and 7 kb. The chromosomal location of the human neogenin gene (HGMW-approved symbol NEO1) was determined as 15q22.3-q23, using fluorescence in situ hybridization. The gene therefore maps in the vicinity of a locus associated with Bardet-Biedl syndrome. The identification of human neogenin and its chromosomal location provides a basis for studying its involvement in genetic disorders or diseases.
journal_name
Genomicsjournal_title
Genomicsauthors
Vielmetter J,Chen XN,Miskevich F,Lane RP,Yamakawa K,Korenberg JR,Dreyer WJdoi
10.1006/geno.1997.4688subject
Has Abstractpub_date
1997-05-01 00:00:00pages
414-21issue
3eissn
0888-7543issn
1089-8646pii
S0888-7543(97)94688-7journal_volume
41pub_type
杂志文章相关文献
GENOMICS文献大全abstract::Consecutive application of PCR and serial analysis of gene expression (SAGE) was used to generate a catalog of approximately 50, 000 SAGEtags from nine human oocytes. Matches for known genes were identified using the National Institutes of Health SAGEtag database. This database links directly to the UniGene database, ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1999.6059
更新日期:2000-01-01 00:00:00
abstract::Genomic duplication, followed by divergence, contributes to organismal evolution. Several mechanisms, such as exon shuffling and alternative splicing, are responsible for novel gene functions, but they generate homologous domains and do not usually lead to drastic innovation. Major novelties can potentially be introdu...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2006.06.009
更新日期:2006-12-01 00:00:00
abstract::We have constructed a genetic linkage map of 17 markers on the long arm of human chromosome 21, including six genes and two anonymous loci with a variable number of tandem repeats. The estimated length of the map is 103 cM in males and 140 cM in females, assuming Kosambi interference. Recombination in females was appr...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(89)90282-6
更新日期:1989-05-01 00:00:00
abstract::The functionality of sense-antisense transcripts (SATs), although widespread throughout the mammalian genome, is largely unknown. Here, we analyzed the SATs expression and its associated promoter DNA methylation status by surveying 12 tissues of mice to gain insights into the relationship between expression and DNA me...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2010.08.007
更新日期:2010-12-01 00:00:00
abstract::We used a combination of sequence analysis and exon trapping in an effort to determine the complete transcript map for a cosmid (6E5) derived from 12q13.3, a region of DNA sequence amplification in human cancers. This cosmid, previously known to contain three genes (CDK4, SAS, and OS9), was sequenced, and that informa...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1997.4727
更新日期:1997-06-01 00:00:00
abstract::For genetic linkage analysis of Japanese flounder, 160 doubled haploids (DH) were artificially produced using mitotic gynogenesis and were genotyped for 458 simple sequence repeat (SSR) markers, 101 of which show distortional segregation. The genetic linkage map was constructed by modifying recombination fractions bet...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2017.08.006
更新日期:2018-01-01 00:00:00
abstract::We have reported that a novel c-Myc binding protein, AMY-1, stimulated the transcription activity of c-Myc and was translocated from the cytoplasm to the nucleus in a c-Myc-dependent manner. AMY-1 works as an inducer of human K562 cell differentiation upon induction of AraC. To characterize the expression or functiona...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/s0888-7543(03)00006-5
更新日期:2003-02-01 00:00:00
abstract::Current microarray technology allows researchers to genotype a large number of SNPs with relatively small amounts of DNA. Nevertheless, researchers and clinicians still frequently face the problem of acquiring enough high-quality DNA for analysis. Whole-genome amplification (WGA) methods offer a solution for this prob...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2008.08.007
更新日期:2008-12-01 00:00:00
abstract::Mutations in the mouse dreher (dr) gene cause skeletal defects, hyperactivity, abnormal gait, deafness, white belly spotting, and hypoplasia of Müllerian duct derivatives. To map dr to high resolution, we utilized two crosses. Initially, we analyzed an intersubspecific intercross to construct a detailed genetic map of...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1999.5873
更新日期:1999-08-01 00:00:00
abstract::RNA helicase A is an enzyme that possesses both RNA and DNA helicase activities. In this report, we describe the isolation of a mouse cDNA encoding RNA helicase A. The deduced amino acid sequence derived from mouse RNA helicase A cDNA exhibits 87 and 47% identity to its human and Drosophila homologs, respectively. Usi...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1997.5139
更新日期:1998-02-01 00:00:00
abstract::We have assigned the human tripeptidyl peptidase II (TPP2) gene to chromosome region 13q32-q33 using two different methods. First, a full-length TPP2 cDNA was used as a probe on Southern blots of DNA from a panel of human/rodent somatic cell hybrids. The TPP2 sequences were found to segregate with the human chromosome...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1993.1353
更新日期:1993-08-01 00:00:00
abstract::Brachyuran crabs comprise the most species-rich clade among the crustacean order Decapoda and are divided into several major superfamilies. However, the monophyly of the superfamilies Ocypodoidea and Grapsoidea in their current compositions within the Brachyura remains inconclusive. In this study, the complete mitocho...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2019.06.004
更新日期:2020-01-01 00:00:00
abstract::Pax-1, a member of a murine multigene family, belongs to the paired box-containing class of developmental control genes first identified in Drosophila. The Pax-1 gene encodes a sequence-specific DNA-binding protein with transcriptional activating properties and has been found to be mutated in the autosomal recessive m...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/s0888-7543(05)80177-6
更新日期:1992-11-01 00:00:00
abstract::The closely linked proline-rich protein (Prp) genes, coding for abundant salivary proteins, are located on distal mouse chromosome 6. They are part of a conserved linkage group that is represented on human chromosome 12p. Two other markers, Ea-10 and Es-12, that were previously unassigned to a chromosome are closely l...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(89)90004-9
更新日期:1989-10-01 00:00:00
abstract::RFX1 is a transacting DNA-binding regulatory factor involved in the control of MHC class II gene expression. RFX2 is a structurally very similar protein with identical DNA binding features. A member of the family of RFX factors is affected in an autosomal recessive disease, MHC class II deficient combined immunodefici...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(92)90052-t
更新日期:1992-08-01 00:00:00
abstract::We have isolated cDNA clones that code for human cytochrome b5. Owing to the high degree of evolutionary conservation of cytochrome b5 sequences and the existence of human and rodent cytochrome b5 processed pseudogenes, we were unable to map unambiguously the chromosomal localization of the human gene(s) by Southern b...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(91)90136-3
更新日期:1991-10-01 00:00:00
abstract::The chromosomal locations and the tissue expression patterns of the human transcriptional adaptors TADA2L and GCN5L2 have been determined. Northern blot analysis across a range of human tissues revealed that both the TADA2L and the GCN5L2 mRNAs are expressed to varying degrees in all tissue types. Furthermore, in most...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1996.4605
更新日期:1997-03-15 00:00:00
abstract::MicroRNAs (miRNAs) are a group of RNAs that play important roles in regulating gene expression and protein translation. In a previous study, we established an oligonucleotide microarray platform to detect miRNA expression. Because it contained only hundreds of probes, data normalization was difficult. In this study, t...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2008.04.002
更新日期:2008-08-01 00:00:00
abstract::Analysis of the exon-intron organization of the human dystrophin gene has been hampered by its enormous size. By using a YAC-based exon mapping approach and long PCR, we have succeeded in defining the size of the gene and its organization. Our results, compared with data on the distribution of deletion breakpoints by ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1997.4911
更新日期:1997-10-15 00:00:00
abstract::The behaviors of autism overlap with a diverse array of other neurological disorders, suggesting common molecular mechanisms. We conducted a large comparative analysis of the network of genes linked to autism with those of 432 other neurological diseases to circumscribe a multi-disorder subcomponent of autism. We leve...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2008.09.015
更新日期:2009-02-01 00:00:00
abstract::The mouse gene encoding glial high-affinity, Na+-dependent glutamate transporter Slc1a3 (GluT-1/GLAST) was isolated, and its structural organization was characterized. The gene appeared to exist as a single copy in the mouse genome and comprised 10 exons spanning more than 56 kilobases. The transcription initiation si...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1996.0226
更新日期:1996-05-01 00:00:00
abstract::N6-methyladenine (6mA) is one kind of post-replication modification (PTM or PTRM) occurring in a wide range of DNA sequences. Accurate identification of its sites will be very helpful for revealing the biological functions of 6mA, but it is time-consuming and expensive to determine them by experiments alone. Unfortuna...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2018.01.005
更新日期:2019-01-01 00:00:00
abstract::In an attempt to identify C/EBP-like transcription factors expressed in the prostate, a cDNA homologous to the mouse C/EBP delta (CRP3) and the rat CELF gene was isolated. A genomic clone containing the entire C/EBP delta gene was isolated using a cDNA fragment as a probe. The gene was characterized by restriction map...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1993.1220
更新日期:1993-05-01 00:00:00
abstract::Helicobacter pylori is a Gram-negative spiral-shaped bacterium that infects half of the human population worldwide and causes chronic inflammation. In the present study, we used the art of computational biology for therapeutic drug targets identification and a multi-epitope vaccine against multi-strains of H. pylori. ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2020.06.026
更新日期:2020-09-01 00:00:00
abstract::We describe here a method for DNA fingerprinting of human chromosomes by Alu-polymerase chain reaction (PCR) amplification of DNA from monochromosomal hybrids, following digestion with restriction endonucleases. DNA digestion with restriction enzymes prior to PCR amplification reduces the total number of amplified fra...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/s0888-7543(05)80175-2
更新日期:1992-11-01 00:00:00
abstract::Alternative splicing is an important cellular mechanism that increases the diversity of gene products. The number of alternatively spliced genes reported so far in plants is much smaller than that in mammals, but is increasing as a result of the explosive growth of available EST and genomic sequences. We have searched...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/s0888-7543(03)00204-0
更新日期:2003-12-01 00:00:00
abstract::Fine mapping followed by candidate gene analysis of erd - a canine hereditary retinal degeneration characterized by aberrant photoreceptor development - established that the disease cosegregates with a SINE insertion in exon 4 of the canine STK38L/NDR2 gene. The mutation removes exon 4 from STK38L transcripts and is p...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2010.09.003
更新日期:2010-12-01 00:00:00
abstract::The association of the HLA class III region with many diseases motivates the investigation of unidentified genes in the 30-kb segment between complement component genes Bf and C4. RD, which codes for a putative RNA binding protein, is 205 bp downstream of Bf. SKI2W (HGMW-approved symbol SKIV2L), a DEVH-box gene probab...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1998.5499
更新日期:1998-11-01 00:00:00
abstract::Madin-Darby canine kidney(MDCK) cells can be used to prepare cell-based influenza vaccines; however, little is known regarding the effect of lncRNA regulatorson tumorigenicity. In the present study, two cell lines with low tumorigenicity were screened from highly tumorigenic MDCK cell lines using monoclonal cell techn...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2019.08.002
更新日期:2020-03-01 00:00:00
abstract::The major component of the vascular and plaque amyloid deposits in Alzheimer disease is the amyloid beta peptide (A beta). A second intrinsic component of amyloid, the NAC (non-A beta component of amyloid) peptide, has recently been identified, and its precursor protein was named NACP. A computer homology search allow...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(95)80208-4
更新日期:1995-03-20 00:00:00