Detection of obesity QTLs on mouse chromosomes 1 and 7 by selective DNA pooling.

Abstract:

:The inheritance of obesity has been analyzed in an intercross between the lean 129/Sv mouse strain and the obesity-prone EL/Suz mouse strain. The weights of three major fat pads were determined on 4-month-old mice, and the sum of these weights, divided by body weight, was used as an adiposity index. The strategy of selective DNA pooling was used as a primary screen to identify putative quantitative trait loci (QTLs) affecting adiposity index. DNA pools representing the leanest 15% and fattest 15% of the F2 progeny were compared for differential allelic enrichment using widely dispersed microsatellite variants. To evaluate putative QTLs, individual genotyping and interval mapping were employed to estimate QTL effects and assess statistical significance. One QTL affecting adiposity index, which accounted for 12.3% of phenotypic variance in gender-merged data, was mapped to the central region of Chromosome (Chr) 7. The QTL allele inherited from EL conferred increased adiposity. A second QTL that accounts for 6.3% of phenotypic variance was identified on Chr 1 near D1Mit211. At both QTLs, the data are consistent with dominant inheritance of the allele contributing to obesity. The possible relationships between these QTLs and previously described obesity QTLs, major obesity mutations, and candidate genes are discussed.

journal_name

Genomics

journal_title

Genomics

authors

Taylor BA,Phillips SJ

doi

10.1006/geno.1996.0302

subject

Has Abstract

pub_date

1996-06-15 00:00:00

pages

389-98

issue

3

eissn

0888-7543

issn

1089-8646

pii

S0888-7543(96)90302-X

journal_volume

34

pub_type

杂志文章

相关文献

GENOMICS文献大全
  • Human retinal guanylate cyclase (GUC2D) maps to chromosome 17p13.1.

    abstract::3',5'-Cyclic guanosine monophosphate is the intracellular second messenger regulating phototransduction in mammals. The level of cGMP in photoreceptor cells is controlled by the cGMP-hydrolyzing enzyme cGMP phosphodiesterase and the cGMP-producing enzyme guanylate cyclase. Identification of a photoreceptor-specific gu...

    journal_title:Genomics

    pub_type: 杂志文章

    doi:10.1006/geno.1994.1415

    authors: Oliveira L,Miniou P,Viegas-Pequignot E,Rozet JM,Dollfus H,Pittler SJ

    更新日期:1994-07-15 00:00:00

  • Two novel genes, Gpr113, which encodes a family 2 G-protein-coupled receptor, and Trcg1, are selectively expressed in taste receptor cells.

    abstract::To identify genes important for taste receptor cell function, we analyzed the sequences and expression patterns of clones isolated from a mouse taste receptor cell-enriched cDNA library. Here, we report the analyses of two novel genes, Gpr113 and Trcg1. Gpr113 encodes a G-protein-coupled receptor belonging to family 2...

    journal_title:Genomics

    pub_type: 杂志文章

    doi:10.1016/j.ygeno.2004.12.005

    authors: LopezJimenez ND,Sainz E,Cavenagh MM,Cruz-Ithier MA,Blackwood CA,Battey JF,Sullivan SL

    更新日期:2005-04-01 00:00:00

  • Cloning of genomic loci and chromosomal localization of the human PCTAIRE-1 and -3 protein kinase genes.

    abstract::Recent studies on the molecular mechanisms controlling the mammalian cell cycle have disclosed a large family of cdc2-related serine/threonine kinases. Among this gene family, the PCTAIRE protein kinases comprise a distinct subfamily of unknown cellular function. To analyze the genomic structure and chromosomal locati...

    journal_title:Genomics

    pub_type: 杂志文章

    doi:10.1006/geno.1994.1245

    authors: Okuda T,Valentine VA,Shapiro DN,Downing JR

    更新日期:1994-05-01 00:00:00

  • RNA-sequencing reveals previously unannotated protein- and microRNA-coding genes expressed in aleurone cells of rice seeds.

    abstract::The rice genome annotation has been greatly improved in recent years, largely due to the availability of full length cDNA sequences derived from many tissues. Among those yet to be studied is the aleurone layer, which produces hydrolases for mobilization of seed storage reserves during seed germination and post germin...

    journal_title:Genomics

    pub_type: 杂志文章

    doi:10.1016/j.ygeno.2013.10.007

    authors: Watanabe KA,Ringler P,Gu L,Shen QJ

    更新日期:2014-01-01 00:00:00

  • Genetic heterogeneity of familial hemiplegic migraine.

    abstract::Familial hemiplegic migraine (FHM) is a distinctive form of migraine with an autosomal dominant mode of inheritance. The migraine-like attacks are associated with transient hemiparesis. A locus for FHM has recently been assigned to chromosome 19 by linkage mapping. In the present study, five unrelated pedigrees with m...

    journal_title:Genomics

    pub_type: 杂志文章

    doi:10.1006/geno.1994.1340

    authors: Ophoff RA,van Eijk R,Sandkuijl LA,Terwindt GM,Grubben CP,Haan J,Lindhout D,Ferrari MD,Frants RR

    更新日期:1994-07-01 00:00:00

  • C-T variant in a miRNA target site of BCL2 is associated with increased risk of human papilloma virus related cervical cancer--an in silico approach.

    abstract::MicroRNAs control gene expression at the posttranscriptional level by base-pairing to the 3'-UTR of their target mRNAs, thus leading to mRNA degradation of protein fabrication. We hypothesize, SNPs within miRNAs and their targets could be of significance to an individual's risk of developing cancer. We analyzed in sil...

    journal_title:Genomics

    pub_type: 杂志文章

    doi:10.1016/j.ygeno.2011.06.005

    authors: Reshmi G,Surya R,Jissa VT,Babu PS,Preethi NR,Santhi WS,Jayaprakash PG,Pillai MR

    更新日期:2011-09-01 00:00:00

  • Whole genome sequencing of pairwise human subjects reveals DNA mutations specific to developmental dysplasia of the hip.

    abstract::Developmental dysplasia of the hip (DDH) is a common congenital malformation characterized by mismatch in shape between the femoral head and acetabulum, and leads to hip dysplasia. To date, the pathogenesis of DDH is poorly understood and may involve multiple factors, including genetic predisposition. However, compreh...

    journal_title:Genomics

    pub_type: 杂志文章

    doi:10.1016/j.ygeno.2018.02.006

    authors: Zhu LQ,Su GH,Dai J,Zhang WY,Yin CH,Zhang FY,Zhu ZH,Guo ZX,Fang JF,Zou CD,Chen XG,Zhang Y,Xu CY,Zhen YF,Wang XD

    更新日期:2019-05-01 00:00:00

  • Fibulin-2 (FBLN2): human cDNA sequence, mRNA expression, and mapping of the gene on human and mouse chromosomes.

    abstract::Fibulin-2 is a new extracellular matrix protein that we recently identified by characterizing mouse cDNA clones. Fibulin-2 mRNA is prominently expressed in mouse heart tissue and is present in low amounts in other tissues. In this study, we isolated and sequenced a 4.1-kb human fibulin-2 cDNA, which encoded a mature p...

    journal_title:Genomics

    pub_type: 杂志文章

    doi:10.1006/geno.1994.1404

    authors: Zhang RZ,Pan TC,Zhang ZY,Mattei MG,Timpl R,Chu ML

    更新日期:1994-07-15 00:00:00

  • Linkage map of nine loci defined by polymorphic DNA markers assigned to rat chromosome 13.

    abstract::A genetic map of nine loci defined by polymorphic DNA markers was created using a single cross of F344/N and LEW/N rats. The markers contained polymorphic simple sequence repeats identified in five genes, renin (Ren), cardiac troponin T (Tnnt3), synaptotagmin (Syt2), Na+,K(+)-ATPase catalytic subunit (Atp1a2), and the...

    journal_title:Genomics

    pub_type: 杂志文章

    doi:10.1006/geno.1993.1466

    authors: Remmers EF,Goldmuntz EA,Zha H,Mathern P,Du Y,Crofford LJ,Wilder RL

    更新日期:1993-11-01 00:00:00

  • Isolation and chromosomal localization of the human glutathione peroxidase gene.

    abstract::We have isolated cDNA clones for the gene, termed GPX1, encoding the major human selenoprotein, glutathione peroxidase. Sequence analysis confirmed previous findings that the unusual amino acid seleno-cysteine is encoded by the opal terminator codon UGA. Southern blot analysis of human genomic DNA with the GPX1 cDNA s...

    journal_title:Genomics

    pub_type: 杂志文章

    doi:10.1016/0888-7543(90)90566-d

    authors: Chada S,Le Beau MM,Casey L,Newburger PE

    更新日期:1990-02-01 00:00:00

  • Evidence of systematic expressed sequence tag IMAGE clone cross-hybridization on cDNA microarrays.

    abstract::We present evidence of a potentially serious source of error intrinsic to all spotted cDNA microarrays that use IMAGE clones of expressed sequence tags (ESTs). We found that a high proportion of these EST sequences contain 5'-end poly(dT) sequences that are remnants from the oligo(dT)-primed reverse transcription of p...

    journal_title:Genomics

    pub_type: 杂志文章

    doi:10.1016/j.ygeno.2003.12.010

    authors: Handley D,Serban N,Peters D,O'Doherty R,Field M,Wasserman L,Spirtes P,Scheines R,Glymour C

    更新日期:2004-06-01 00:00:00

  • Identification of a doublet missense substitution in the bovine LRP4 gene as a candidate causal mutation for syndactyly in Holstein cattle.

    abstract::Syndactyly in Holstein cattle is an autosomal recessive abnormality characterized by the fusion of the functional digits. This disorder has been previously mapped to the telomeric part of bovine chromosome 15. Here, we describe the fine-mapping of syndactyly in Holstein cattle to a 3.5-Mb critical interval using a com...

    journal_title:Genomics

    pub_type: 杂志文章

    doi:10.1016/j.ygeno.2006.05.007

    authors: Duchesne A,Gautier M,Chadi S,Grohs C,Floriot S,Gallard Y,Caste G,Ducos A,Eggen A

    更新日期:2006-11-01 00:00:00

  • Odorant and vomeronasal receptor genes in two mouse genome assemblies.

    abstract::Odorant receptors (ORs) and vomeronasal receptors (V1Rs and V2Rs) are large superfamilies of chemosensory receptors. As an extension of previous research using the 2001 Celera mouse genome assembly, we analyzed OR and V1R genes in the 2002 public mouse genome assembly. We identified 1403 OR genes (1068 potentially int...

    journal_title:Genomics

    pub_type: 杂志文章

    doi:10.1016/j.ygeno.2003.10.009

    authors: Zhang X,Rodriguez I,Mombaerts P,Firestein S

    更新日期:2004-05-01 00:00:00

  • Mapping of the genes encoding human inducible and endothelial nitric oxide synthase (NOS2 and NOS3) to the pericentric region of chromosome 17 and to chromosome 7, respectively.

    abstract::Nitric oxide (NO) is an important molecular messenger regulating the functions of a wide variety of cells and tissues. NO is synthesized from L-arginine by a variety of isoforms of the enzyme nitric oxide synthase (NOS). We have used Southern blotting analysis on DNAs obtained from a panel of human-rodent hybrid cell ...

    journal_title:Genomics

    pub_type: 杂志文章

    doi:10.1006/geno.1994.1286

    authors: Xu W,Charles IG,Moncada S,Gorman P,Sheer D,Liu L,Emson P

    更新日期:1994-05-15 00:00:00

  • Conservation of position and sequence of a novel, widely expressed gene containing the major human alpha-globin regulatory element.

    abstract::We have determined the cDNA and genomic structure of a gene (-14 gene) that lies adjacent to the human alpha-globin cluster. Although it is expressed in a wide range of cell lines and tissues, a previously described erythroid-specific regulatory element that controls expression of the alpha-globin genes lies within in...

    journal_title:Genomics

    pub_type: 杂志文章

    doi:10.1006/geno.1995.9951

    authors: Vyas P,Vickers MA,Picketts DJ,Higgs DR

    更新日期:1995-10-10 00:00:00

  • DNA methylation microarray uncovers a permissive methylome for cardiomyocyte differentiation in human mesenchymal stem cells.

    abstract::Differentiation of Wharton's Jelly-Mesenchymal Stem cells (WJ-MSCs) into cardiomyocytes (CMs) in vitro has been reported widely although contradictions remain regarding the maturation of differentiated MSCs into fully functioning CMs. Studies suggest that use of epigenetic modifiers like 5'Azacytidine (5-AC) in MSCs d...

    journal_title:Genomics

    pub_type: 杂志文章

    doi:10.1016/j.ygeno.2019.08.007

    authors: Govarthanan K,Gupta PK,Ramasamy D,Kumar P,Mahadevan S,Verma RS

    更新日期:2020-03-01 00:00:00

  • Syntenic assignments of visual transduction genes in cattle.

    abstract::To establish syntenic relationships of phototransduction genes, we have mapped the genes encoding the alpha-, beta-, and gamma-subunits of rod cGMP phosphodiesterase (PDE) (PDEA, PDEB, PDEG), the alpha'-subunit of cone PDE (PDEA2), and the rod cGMP-gated channel (CNCG) to bovine syntenic groups. The rod cGMP PDE alpha...

    journal_title:Genomics

    pub_type: 杂志文章

    doi:10.1016/s0888-7543(05)80171-5

    authors: Gallagher DS Jr,Womack JE,Baehr W,Pittler SJ

    更新日期:1992-11-01 00:00:00

  • The CD39-like gene family: identification of three new human members (CD39L2, CD39L3, and CD39L4), their murine homologues, and a member of the gene family from Drosophila melanogaster.

    abstract::The human lymphoid cell activation antigen CD39 is a known E-type apyrase that hydrolyzes extracellular ATP and ADP, a function important in homotypic adhesion, platelet aggregation, and removal by activated lymphocytes of the lytic effect of ATP. The recently identified putative rat homologue of CD39L1 has been shown...

    journal_title:Genomics

    pub_type: 杂志文章

    doi:10.1006/geno.1998.5317

    authors: Chadwick BP,Frischauf AM

    更新日期:1998-06-15 00:00:00

  • Comparative mapping in the beige-satin region of mouse chromosome 13.

    abstract::The proximal end of mouse chromosome (Chr) 13 contains regions conserved on human chromosomes 1q42-q44, 6p23-p21, and 7p22-p13. This region also contains mutations that may be models for human disease, including beige (human Chediak-Higashi syndrome). An interspecific backcross of SB/Le and Mus spretus mice was used t...

    journal_title:Genomics

    pub_type: 杂志文章

    doi:10.1006/geno.1996.4478

    authors: Perou CM,Perchellet A,Jago T,Pryor R,Kaplan J,Justice MJ

    更新日期:1997-01-15 00:00:00

  • The complete nucleotide sequence of murine beta-glucuronidase mRNA and its deduced polypeptide.

    abstract::The complete nucleotide sequence of murine beta-glucuronidase (GUS) mRNA has been compiled from three overlapping cloned cDNAs and a single GUS-specific genomic clone. The sequence is composed of 2455 nucleotides, exclusive of the poly(A) tail. The 5' and 3' untranslated regions contain 12 and 499 bases, respectively,...

    journal_title:Genomics

    pub_type: 杂志文章

    doi:10.1016/0888-7543(88)90005-5

    authors: Gallagher PM,D'Amore MA,Lund SD,Ganschow RE

    更新日期:1988-04-01 00:00:00

  • A physical and transcriptional map of the preaxial polydactyly locus on chromosome 7q36.

    abstract::Preaxial polydactyly is a congenital hand malformation that includes duplicated thumbs, various forms of triphalangeal thumbs, and duplications of the index finger. A locus for preaxial polydactyly has been mapped to a region of 1.9 cM on chromosome 7q36 between polymorphic markers D7S550 and D7S2423. We constructed a...

    journal_title:Genomics

    pub_type: 杂志文章

    doi:10.1006/geno.1999.5796

    authors: Heus HC,Hing A,van Baren MJ,Joosse M,Breedveld GJ,Wang JC,Burgess A,Donnis-Keller H,Berglund C,Zguricas J,Scherer SW,Rommens JM,Oostra BA,Heutink P

    更新日期:1999-05-01 00:00:00

  • Four paralogous protein 4.1 genes map to distinct chromosomes in mouse and human.

    abstract::Four highly conserved members of the skeletal protein 4.1 gene family encode a diverse array of protein isoforms via tissue-specific transcription and developmentally regulated alternative pre-mRNA splicing. In addition to the prototypical red blood cell 4.1R (human gene symbol EPB41,) these include two homologues tha...

    journal_title:Genomics

    pub_type: 杂志文章

    doi:10.1006/geno.1998.5537

    authors: Peters LL,Weier HU,Walensky LD,Snyder SH,Parra M,Mohandas N,Conboy JG

    更新日期:1998-12-01 00:00:00

  • The human alpha 2(XI) collagen gene (COL11A2) maps to the centromeric border of the major histocompatibility complex on chromosome 6.

    abstract::Type XI collagen, a minor structural component of cartilage fibrils, is composed of three chains, alpha 1(XI), alpha 2(XI), and alpha 3(XI). Using a cloned fragment of the human alpha 2(XI) collagen gene (COL11A2) as a molecular probe for in situ hybridization and somatic cell hybrid mapping, we have localized the gen...

    journal_title:Genomics

    pub_type: 杂志文章

    doi:10.1016/0888-7543(89)90135-3

    authors: Hanson IM,Gorman P,Lui VC,Cheah KS,Solomon E,Trowsdale J

    更新日期:1989-11-01 00:00:00

  • Database and analyses of known alternatively spliced genes in plants.

    abstract::Alternative splicing is an important cellular mechanism that increases the diversity of gene products. The number of alternatively spliced genes reported so far in plants is much smaller than that in mammals, but is increasing as a result of the explosive growth of available EST and genomic sequences. We have searched...

    journal_title:Genomics

    pub_type: 杂志文章

    doi:10.1016/s0888-7543(03)00204-0

    authors: Zhou Y,Zhou C,Ye L,Dong J,Xu H,Cai L,Zhang L,Wei L

    更新日期:2003-12-01 00:00:00

  • Functional analysis of the murine Emr1 promoter identifies a novel purine-rich regulatory motif required for high-level gene expression in macrophages.

    abstract::This study has investigated the transcriptional regulation of the Emr1 gene in murine macrophages and defined an enhancer element within the proximal promoter that is necessary for Emr1 expression in myeloid cells. This element consists of an extended purine-rich sequence (PuRS) of 83 consecutive purine residues conta...

    journal_title:Genomics

    pub_type: 杂志文章

    doi:10.1016/j.ygeno.2004.08.016

    authors: O'Reilly D,Addley M,Quinn C,MacFarlane AJ,Gordon S,McKnight AJ,Greaves DR

    更新日期:2004-12-01 00:00:00

  • Delineation of 7q11.2 deletions associated with Williams-Beuren syndrome and mapping of a repetitive sequence to within and to either side of the common deletion.

    abstract::The majority of Williams-Beuren syndrome (WBS) patients have been shown to have a microdeletion within 7q11.2 including the elastin gene locus. The extent of these deletions has, however, not been well characterized. Thirty-five deletion patients were tested for all polymorphic markers in the 7q11.2 region bounding EL...

    journal_title:Genomics

    pub_type: 杂志文章

    doi:10.1006/geno.1996.0237

    authors: Robinson WP,Waslynka J,Bernasconi F,Wang M,Clark S,Kotzot D,Schinzel A

    更新日期:1996-05-15 00:00:00

  • A primary genetic map of the pericentromeric region of the human X chromosome.

    abstract::We report a genetic linkage map of the pericentromeric region of the human X chromosome, extending from Xp11 to Xq13. Genetic analysis with five polymorphic markers, including centromeric alpha satellite DNA, spanned a distance of approximately 38 cM. Significant lod scores were obtained with linkage analysis in 26 fa...

    journal_title:Genomics

    pub_type: 杂志文章

    doi:10.1016/0888-7543(88)90017-1

    authors: Mahtani MM,Willard HF

    更新日期:1988-05-01 00:00:00

  • Chromosomal localization of the human gene encoding c-myc promoter-binding protein (MPB1) to chromosome 1p35-pter.

    abstract::We report the mapping of the human gene MPB1 (c-myc promoter binding protein), a recently identified gene regulatory protein. MPB1 binds to the c-myc P2 promoter and exerts a negative regulatory role on c-myc transcription. Since exogenous expression from transfection of the MPB1 gene suppresses the tumorigenic proper...

    journal_title:Genomics

    pub_type: 杂志文章

    doi:10.1006/geno.1996.4499

    authors: White RA,Adkison LR,Dowler LL,Ray RB

    更新日期:1997-02-01 00:00:00

  • Molecular cloning, cDNA sequence analysis, and chromosomal localization of mouse Pkd2.

    abstract::The gene responsible for the second form of autosomal dominant polycystic kidney disease, PKD2, has recently been identified. We now describe the cloning, genomic localization, cDNA sequence, and expression analysis of its murine homologue, Pkd2. The cloned cDNA sequence is 5134 bp long and is predicted to encode a 96...

    journal_title:Genomics

    pub_type: 杂志文章

    doi:10.1006/geno.1997.4920

    authors: Wu G,Mochizuki T,Le TC,Cai Y,Hayashi T,Reynolds DM,Somlo S

    更新日期:1997-10-01 00:00:00

  • Exonic SINE insertion in STK38L causes canine early retinal degeneration (erd).

    abstract::Fine mapping followed by candidate gene analysis of erd - a canine hereditary retinal degeneration characterized by aberrant photoreceptor development - established that the disease cosegregates with a SINE insertion in exon 4 of the canine STK38L/NDR2 gene. The mutation removes exon 4 from STK38L transcripts and is p...

    journal_title:Genomics

    pub_type: 杂志文章

    doi:10.1016/j.ygeno.2010.09.003

    authors: Goldstein O,Kukekova AV,Aguirre GD,Acland GM

    更新日期:2010-12-01 00:00:00