Abstract:
:We have isolated cDNA clones that code for human cytochrome b5. Owing to the high degree of evolutionary conservation of cytochrome b5 sequences and the existence of human and rodent cytochrome b5 processed pseudogenes, we were unable to map unambiguously the chromosomal localization of the human gene(s) by Southern blot hybridization of DNA from human-rodent somatic cell hybrids. An alternative approach, based on restriction enzyme digestion of PCR-amplified DNA, enabled us to map the human cytochrome b5 gene(s) to chromosome 18 and one of its processed pseudogenes to the X chromosome. We propose the designations CYB5 and CYB5P1 for the gene and pseudogene loci, respectively.
journal_name
Genomicsjournal_title
Genomicsauthors
Shephard EA,Povey S,Spurr NK,Phillips IRdoi
10.1016/0888-7543(91)90136-3subject
Has Abstractpub_date
1991-10-01 00:00:00pages
302-8issue
2eissn
0888-7543issn
1089-8646pii
0888-7543(91)90136-3journal_volume
11pub_type
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