Abstract:
:Thirty-eight kilobases of mouse genomic DNA which surround and include the coding sequences for beta-glucuronidase has been mapped. Intron-exon arrangements were determined by hybridization of genomic sequences with cDNA clones, and minimum estimates of gene length (11-17 kb) and intron number were obtained. Only a single gene was observed when genomic DNA was probed with subclones containing beta-glucuronidase coding sequence; there was no evidence of duplicated or pseudogenes. However, sequences distal to the 3' end of the gene are present elsewhere in the genome in a limited number of copies. Eight haplotypes of the beta-glucuronidase region with differing regulatory genotypes were compared for restriction fragment polymorphisms. Surprisingly little was found, considering the diverse origin of the haplotypes. Two of the polymorphisms that were found may be correlated with regulatory phenotypes. A BamHI site is missing from the CS and CL haplotypes that share regulatory properties, and a 0.2-kb insertion is consistently present in haplotypes showing increased response to induction by androgens in kidney.
journal_name
Genomicsjournal_title
Genomicsauthors
Moore KJ,Paigen Kdoi
10.1016/0888-7543(88)90105-xsubject
Has Abstractpub_date
1988-01-01 00:00:00pages
25-31issue
1eissn
0888-7543issn
1089-8646pii
0888-7543(88)90105-Xjournal_volume
2pub_type
杂志文章相关文献
GENOMICS文献大全abstract::Uroporphyrinogen-III (URO) synthase is the heme biosynthetic enzyme defective in congenital erythropoietic porphyria. The approximately 34-kb human URO-synthase gene (UROS) was isolated, and its organization and tissue-specific expression were determined. The gene had two promoters that generated housekeeping and eryt...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.2000.6373
更新日期:2000-12-01 00:00:00
abstract::RIMs are synaptic proteins that are essential for normal neurotransmitter release. We now show that while invertebrates contain only a single RIM gene, vertebrates contain four: two large genes encoding RIM1alpha (0.50 Mb) or RIM2alpha, 2beta, and 2gamma (0.50-0.75 Mb) and two smaller genes encoding RIM3gamma (14 kb) ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/s0888-7543(02)00024-1
更新日期:2003-02-01 00:00:00
abstract::We have mapped the mouse protein tyrosine phosphatase epsilon (PTP epsilon, gene symbol Ptpre) gene to the distal region of chromosome 7 by linkage analysis using two sets of multilocus genetic crosses. The human PTP epsilon gene (gene symbol PTPRE) was mapped to chromosome 10q26 by fluorescence in situ hybridization....
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1996.0061
更新日期:1996-02-01 00:00:00
abstract::In this study, selenocystine, a nutritionally available selenoamino acid, was identified for the first time as a novel agent with anti proliferative activity on human keloids. The 20 μM concentration after 48 h treatment used here was the most effective to reduce keloid fibroblast growth. We analyzed the gene expressi...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2011.02.009
更新日期:2011-05-01 00:00:00
abstract::The human gene encoding the muscle-specific beta-enolase has been isolated. The beta-enolase gene was mapped to chromosome 17 by analysis of a panel of rodent-human somatic cell hybrids. The gene was further localized to the short arm and tentatively to the region 17pter-p11 by analysis of cell hybrids and transfectan...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(90)90467-9
更新日期:1990-01-01 00:00:00
abstract::The chromosomal region 17q12-q21 contains a gene (BRCA1) conferring susceptibility to early-onset familial breast and ovarian cancer. An 8000-rad radiation-reduced hybrid (RH) panel was constructed to provide a resource for long-range mapping of this region. A large fraction of the hybrids (approximately 90%) retained...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1993.1383
更新日期:1993-09-01 00:00:00
abstract::In this report the identification, structure, and chromosomal localization of the human neurogranin gene (NRGN) are described. NRGN is the human homolog of the rat Ng/RC3 gene, which encodes a brain-specific protein expressed in telencephalic neurons. The human NRGN gene spans approximately 12 kb and contains four exo...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1997.4622
更新日期:1997-04-15 00:00:00
abstract::A cosmid library has been constructed with DNA isolated from a mouse/human hybrid cell line designated A15, which was previously characterized and shown to retain chromosome 15 as the only human material. The library was generated and stored as 34 independent pools of primary colonies at 8-10,000 colonies per pool. Sc...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1996.4517
更新日期:1997-02-15 00:00:00
abstract::Macrophage migration inhibitory factor (MIF) was originally identified as a lymphokine. However, recent work strongly suggests a wider role for MIF beyond the immune system. It is expressed specifically in the differentiating cells of the immunologically privileged eye lens and brain, is a delayed early response gene ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1994.1011
更新日期:1994-01-01 00:00:00
abstract::Type XI collagen, a minor structural component of cartilage fibrils, is composed of three chains, alpha 1(XI), alpha 2(XI), and alpha 3(XI). Using a cloned fragment of the human alpha 2(XI) collagen gene (COL11A2) as a molecular probe for in situ hybridization and somatic cell hybrid mapping, we have localized the gen...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(89)90135-3
更新日期:1989-11-01 00:00:00
abstract::We report the cDNA sequence for the bovine gene for fibrillin corresponding to the human gene, fibrillin 1 (FBN1), and the localization of the gene to bovine chromosome 10 (syntenic group U5). The identity between the human and bovine sequences is 97.8% at the amino acid level and 92% at the nucleotide level. The bovi...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1994.1527
更新日期:1994-09-15 00:00:00
abstract::Genes homologous to those located on human chromosome 4 (HSA4) were mapped in the bovine to determine regions of syntenic conservation among humans, mice, and cattle. Previous studies have shown that two homologs of genes on HSA4, PGM2 and PEPS, are located in bovine syntenic group U15 (chromosome 6). The homologous m...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/s0888-7543(05)80295-2
更新日期:1992-09-01 00:00:00
abstract::The genes encoding the alpha 1 chain of Type III collagen (COL3A1) and the alpha 2 chain of Type V (COL5A2) collagen have been mapped to the long arm of human chromosome 2. Linkage analysis in CEPH families indicated that these two genes are close to each other, with no recombination in 37 informative meioses. In the ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(90)90302-b
更新日期:1990-10-01 00:00:00
abstract:BACKGROUND:Renal fibrosis is a final common pathway of chronic kidney disease. SIRT1, a NAD+-dependent protein deacetylase, deacetylates the p65 of NF-κB and shows protective effects in kidney disorders. miR-373 directly targets the 3'UTR of SIRT1. However, roles of miR-373 in renal fibrosis are unclear. METHODS:TGF-β...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2018.04.017
更新日期:2019-07-01 00:00:00
abstract::A human clone corresponding to the gene for the DNA-binding factor LFB3, a protein highly homologous to the liver-specific transcription factor LFB1, has been isolated and partially sequenced. This gene is designated TCF2. Oligonucleotide primers have been designed for LFB3 and used to amplify specifically the human g...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(90)90239-q
更新日期:1990-09-01 00:00:00
abstract::Identification of all the transcription factors (TFs) encoded in a given genome is a prerequisite for understanding transcriptional regulatory networks. Archaea are prokaryotes that constitute one of the three main branches of organisms with an astounding diversity of habitats. In this report, we establish the Archaea...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2007.09.007
更新日期:2008-01-01 00:00:00
abstract::Fragrance is an important component of end-use quality in rice. A set of 516 fragrant rice accessions were genotyped and over 80% of them carried the badh2.7 allele. A subset of 144 mostly fragrant accessions, including nine of Oryza rufipogon, was then subjected to a detailed diversity and haplotype analysis. The lev...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2012.11.010
更新日期:2013-02-01 00:00:00
abstract::Among its known functions, tumor suppressor gene p53 serves as a transcriptional regulator and mediates various signals through activation of downstream genes. We recently identified a novel gene, GML (glycosylphosphatidylinositol (GPI)-anchored molecule-like protein), whose expression is specifically induced by wildt...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1997.4680
更新日期:1997-05-01 00:00:00
abstract::TaqMAMA combines the quantitative strengths of TaqMan with the allele-specific PCR of MAMA. In this article we develop TaqMAMA as a technique for screening human DNA samples for known genetic polymorphisms. In the first set of experiments, plasmids that model all types of genetic polymorphisms were used to understand ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2003.08.005
更新日期:2004-02-01 00:00:00
abstract::We have cloned a basic helix-loop-helix (bHLH) factor gene, Bhlhb4, from a mouse beta-cell line. Fluorescence in situ hybridization (FISH) and genetic mapping place Bhlhb4 at the telomeric end of mouse chromosome 2 (H3-H4), syntenic to human chromosome 20q13. Based on phylogenetic analysis, BHLHB4 belongs to a new sub...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.2002.6708
更新日期:2002-03-01 00:00:00
abstract::Understanding ribosomal protein gene regulation provides a good avenue for understanding gene regulatory networks. Even after 5 decades of research on ribosomal protein gene regulation, little is known about how higher eukaryotic ribosomal protein genes are coordinately regulated at the transcriptional level. However,...
journal_title:Genomics
pub_type: 杂志文章,评审
doi:10.1016/j.ygeno.2007.07.003
更新日期:2007-10-01 00:00:00
abstract::LBR (lamin B receptor) is an integral protein of the inner nuclear membrane encoded by a gene on human chromosome 1q42.1. LBR has a nucleoplasmic, amino-terminal domain of approximately 200 amino acids followed by a carboxyl-terminal domain similar in sequence to yeast and plant sterol reductases. We have determined t...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1998.5615
更新日期:1998-12-15 00:00:00
abstract::A genetic map of nine loci defined by polymorphic DNA markers was created using a single cross of F344/N and LEW/N rats. The markers contained polymorphic simple sequence repeats identified in five genes, renin (Ren), cardiac troponin T (Tnnt3), synaptotagmin (Syt2), Na+,K(+)-ATPase catalytic subunit (Atp1a2), and the...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1993.1466
更新日期:1993-11-01 00:00:00
abstract::A genetic basis for neural tube defects (NTD) is rarely doubted, but the genes involved have not yet been identified. This is partly due to a lack of suitable families on which to perform linkage analysis. An alternative approach is to use the many mouse genes that cause NTD as a means of isolating their human homolog...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(95)80165-i
更新日期:1995-04-10 00:00:00
abstract::Changing bovine milk fatty acid (FA) composition through selection can decrease saturated FA (SFA) consumption, improve human health and provide a means for manipulating processing properties of milk. Our study determined associations between milk FA composition and genes from triacylglycerol (TAG) biosynthesis pathwa...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2014.10.001
更新日期:2014-12-01 00:00:00
abstract::A variety of cellular proteins have been found to bind to related DNA sequences in the enhancer elements of the human immunodeficiency virus, the kappa immunoglobulin gene, the class I major histocompatibility complex gene, and the beta-interferon gene. Recently, lambda gt11 gene expression cloning using ligated oligo...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(91)90371-k
更新日期:1991-04-01 00:00:00
abstract::In the course of a systematic survey of DMD and BMD patients with intronic probes and with cDNA probes covering three-fourths of the coding sequence, 45 molecular deletions within the DMD gene were investigated. Forty-two percent of the breakpoints were located in the intronic sequence containing probe P20, whereas th...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(89)90025-6
更新日期:1989-10-01 00:00:00
abstract::Farletuzumab (FAR) is a humanized monoclonal antibody (mAb) that binds to folate receptor alpha. A Ph3 trial in ovarian cancer patients treated with carboplatin/taxane plus FAR or placebo did not meet the primary statistical endpoint. Subgroup analysis demonstrated that subjects with high FAR exposure levels (Cmin>57....
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2017.04.006
更新日期:2017-07-01 00:00:00
abstract::DNA methylation is a major epigenetic modification of the genome that affects basic biological functions, such as gene expression and cell development. We used the human genome sequences and the DNA methylation data that are available in order to establish a map of the levels of GC and methylation in isochores. We als...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2009.09.006
更新日期:2010-01-01 00:00:00
abstract::Familial hemiplegic migraine (FHM) is a distinctive form of migraine with an autosomal dominant mode of inheritance. The migraine-like attacks are associated with transient hemiparesis. A locus for FHM has recently been assigned to chromosome 19 by linkage mapping. In the present study, five unrelated pedigrees with m...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1994.1340
更新日期:1994-07-01 00:00:00