Abstract:
:The Trp-Asp (WD) motif has been shown to exist in a number of proteins. Genes containing repeats of the WD motif compose a large gene family associated with a variety of cellular functions and can be divided into a number of functional subfamilies. By means of the differential display method using ttw, a mouse model for the early stage of ectopic ossification, we have identified a novel mouse gene, Wdr8 (WD repeat domain 8), which contains two WD repeats, together with its human orthologue. The human and mouse WDR8 genes encode 460 and 462 amino acids, respectively, with 89% identity, and are expressed in almost all tissues, including bone and cartilage, and in bone-forming cells, including osteoblasts and chondrocytes. Wdr8 expression in cartilage was differentially displayed by stimuli for ectopic ossification in ttw and was observed strongly only at a transition period from hypertrophic to mineralizing stages in ATDC5, a chondrogenic cell line that exhibits endochondral ossification, suggesting a potential role for Wdr8 in the process of ossification. The WDR8 protein is highly conserved among a variety of species, but is distinctly different from other WD-repeat proteins, indicating that it represents a novel subfamily of the WD-repeat gene family.
journal_name
Genomicsjournal_title
Genomicsauthors
Koshizuka Y,Ikegawa S,Sano M,Nakamura K,Nakamura Ydoi
10.1006/geno.2000.6475subject
Has Abstractpub_date
2001-03-15 00:00:00pages
252-9issue
3eissn
0888-7543issn
1089-8646pii
S0888-7543(00)96475-9journal_volume
72pub_type
杂志文章相关文献
GENOMICS文献大全abstract::Macrophage migration inhibitory factor (MIF) was originally identified as a lymphokine. However, recent work strongly suggests a wider role for MIF beyond the immune system. It is expressed specifically in the differentiating cells of the immunologically privileged eye lens and brain, is a delayed early response gene ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1994.1011
更新日期:1994-01-01 00:00:00
abstract::Members of the solute carrier family 25 (SLC25) are known to transport molecules over the mitochondrial membrane. In this paper we present 14 novel members of SLC25 family in human. These were provided with following gene symbols by the HGNC: SLC25A32, SLC25A33, SLC25A34, SLC25A35, SLC25A37, SLC25A38, SLC25A39, SLC25A...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2006.06.016
更新日期:2006-12-01 00:00:00
abstract::The structural gene for beta-galactoside-binding protein (Lgals-1), a cell growth regulatory molecule and cystostatic factor, is assigned to the E-region of mouse chromosome 15 and to the region q12-q13.1 of human chromosome 22. The evolutionary conservation of these two regions has been previously suggested from comp...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1993.1039
更新日期:1993-01-01 00:00:00
abstract::We examined the allele and haplotype frequencies of five polymorphic DNA markers in 355 European cystic fibrosis (CF) patients (from Belgium, the German Democratic Republic, Greece, and Italy) who were divided into two groups according to whether they were or not taking supplementary pancreatic enzymes. The level of l...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(89)90131-6
更新日期:1989-11-01 00:00:00
abstract::Single nucleotide polymorphism (SNP) interactions can explain the missing heritability of common complex diseases. Many interaction detection methods have been proposed in genome-wide association studies, and they can be divided into two types: population-based and family-based. Compared with population-based methods,...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2018.07.014
更新日期:2019-09-01 00:00:00
abstract::Differentiation of Wharton's Jelly-Mesenchymal Stem cells (WJ-MSCs) into cardiomyocytes (CMs) in vitro has been reported widely although contradictions remain regarding the maturation of differentiated MSCs into fully functioning CMs. Studies suggest that use of epigenetic modifiers like 5'Azacytidine (5-AC) in MSCs d...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2019.08.007
更新日期:2020-03-01 00:00:00
abstract::The single functional mouse gene for MIF (macrophage migration inhibitory factor) has been cloned from a P1 library, and its exon/intron structure determined and shown to resemble that of the human gene. The gene was mapped to chromosome 10 using two multilocus crosses between laboratory strains and either Mus musculu...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1995.1070
更新日期:1995-06-10 00:00:00
abstract::Despite the broad variety of available microRNA (miRNA) prediction tools, their application to the discovery and annotation of novel miRNA genes in domestic species is still limited. In this study we designed a comprehensive pipeline (eMIRNA) for miRNA identification in the yet poorly annotated porcine genome and demo...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2019.12.005
更新日期:2020-05-01 00:00:00
abstract::Interspersed repetitive sequence (IRS) PCR and repetitive element-to-bubble (IRS-bubble) PCR have been utilized to rapidly generate large numbers of mouse-specific, chromosome 1-enriched STSs from mouse-hamster somatic cell hybrids. Single-strand conformational polymorphism (SSCP) has been used to localize 39 new repe...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/s0888-7543(11)80007-8
更新日期:1993-12-01 00:00:00
abstract::Multiple infections by HPV genotypes are frequently detected in HPV+ cervical lesions but the interaction between each viral genotype during carcinogenesis is poorly understood. Here we carried out a comprehensive study to characterize the multiple HPV expression and integration by RNA-seq analyses of 19 invasive cerv...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2020.05.009
更新日期:2020-09-01 00:00:00
abstract::We have cloned a basic helix-loop-helix (bHLH) factor gene, Bhlhb4, from a mouse beta-cell line. Fluorescence in situ hybridization (FISH) and genetic mapping place Bhlhb4 at the telomeric end of mouse chromosome 2 (H3-H4), syntenic to human chromosome 20q13. Based on phylogenetic analysis, BHLHB4 belongs to a new sub...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.2002.6708
更新日期:2002-03-01 00:00:00
abstract::Mammalian genomes include DNA segments that are imprinted (CpG-methylated) only on one of the two parental chromosomes, leading to parent-of-origin-specific gene expression. The process is regulated by Imprinting Control Regions (ICRs) and germline Differentially Methylated Regions (gDMRs). Previously, ZFP57 was shown...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2017.04.008
更新日期:2017-07-01 00:00:00
abstract::WRKY proteins constitute a large family of transcription factors. In this study, we identified 81 WRKY genes (named HbWRKY1 to HbWRKY81) in the latest rubber tree genome. Tissue-specific expression profiles showed that 74 HbWRKYs were expressed in at least one of the tissues and the other 7 genes showed very low expre...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2014.04.004
更新日期:2014-07-01 00:00:00
abstract::One hundred nineteen YACs were assembled into 6 contigs spanning about 7.1 Mb of Xq28. The contigs were formatted with 65 STSs and 136 hybridization probes and were extensive enough to be aligned and oriented by published genetic linkage and somatic cell hybrid panel data. Selected YACs from the entire region were map...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1994.1592
更新日期:1994-11-01 00:00:00
abstract::The proximal end of mouse chromosome (Chr) 13 contains regions conserved on human chromosomes 1q42-q44, 6p23-p21, and 7p22-p13. This region also contains mutations that may be models for human disease, including beige (human Chediak-Higashi syndrome). An interspecific backcross of SB/Le and Mus spretus mice was used t...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1996.4478
更新日期:1997-01-15 00:00:00
abstract::In line 4 transgenic mice, six to eight copies of a 50-kb lambda recombinant clone are arranged in a head-to-tail tandem array on chromosome 3. Embryos homozygous for the transgene become arrested in their development on Day 5 of gestation shortly after implantation. The insertion site was cloned using a small segment...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(92)90216-f
更新日期:1992-05-01 00:00:00
abstract::Chimpanzees are naturally and asymptomatically infected by simian immunodeficiency virus (SIV). Pathogenic properties of SIV/HIV vary and differences in susceptibility and pathogenicity of SIV/HIV depend in part on host-specific factors such as virus-receptor/co-receptor interactions. Since CD4 plays a primary role in...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2008.07.003
更新日期:2008-11-01 00:00:00
abstract::Uroporphyrinogen-III (URO) synthase is the heme biosynthetic enzyme defective in congenital erythropoietic porphyria. The approximately 34-kb human URO-synthase gene (UROS) was isolated, and its organization and tissue-specific expression were determined. The gene had two promoters that generated housekeeping and eryt...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.2000.6373
更新日期:2000-12-01 00:00:00
abstract::The procollagen C-proteinase enhancer (PCPE) is a glycoprotein that potentiates enzymatic cleavage of the type I procollagen C-propeptide by bone morphogenetic protein-1 (BMP-1). The human PCPE gene (PCOLCE) was previously mapped to 7q22, an area frequently disrupted in uterine leiomyomata, while disruption of the rat...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1998.5663
更新日期:1999-01-15 00:00:00
abstract::The autosomal recessive mutation mnd2 results in early onset motor neuron disease with rapidly progressive paralysis, severe muscle wasting, regression of thymus and spleen, and death before 40 days of age. mnd2 has been mapped to mouse chromosome 6 with the gene order: centromere-Tcrb-Ly-2-Sftp-3-D6Mit4-mnd2-D6Mit 6,...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1993.1246
更新日期:1993-06-01 00:00:00
abstract::Physical maps can be constructed by "fingerprinting" a large number of random clones and inferring overlap between clones when the fingerprints are sufficiently similar. E. Lander and M. Waterman (Genomics 2: 231-239, 1988) gave a mathematical analysis of such mapping strategies. The analysis is useful for comparing v...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(95)80086-2
更新日期:1995-03-01 00:00:00
abstract::Unbalanced chromosome translocations with breakpoints around 8p12, resulting in loss of distal 8p, are common in carcinomas. We have mapped the 8p12 breakpoints in three breast cancer cell lines, T-47D, MDA-MB-361, and ZR-75-1, using YACs and PACs between D8S540 and D8S255 by fluorescence in situ hybridization. All th...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.2000.6178
更新日期:2000-05-15 00:00:00
abstract::Despite antibiotic therapy and vaccination programs, microbial diseases continue to be the leading cause of morbidity and mortality worldwide. The genetic basis of the host response to infection is complex, and its understanding has been facilitated through the study of mouse models of human infectious diseases. Genet...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1997.5116
更新日期:1998-01-15 00:00:00
abstract::Activating mutations in the ras genes are commonly found in a wide range of human tumors. We recently cloned two mammalian genes, Son of sevenless 1 (mSos1) and Son of sevenless 2 (mSos2), whose protein products appear to be important positive regulators of ras proteins. Given the proposed role of Sos proteins in ras ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1993.1421
更新日期:1993-10-01 00:00:00
abstract::Biologic effects of androgen on target cells are mediated in part by transcriptional regulation of androgen-regulated genes (ARGs) by androgen receptor. Using serial analysis of gene expression (SAGE), we have identified a comprehensive repertoire of ARGs in LNCaP cells. One of the SAGE-derived tags exhibiting homolog...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.2000.6214
更新日期:2000-06-15 00:00:00
abstract::A contig of 20 yeast artificial clones (YACs) has been assembled across 1.5 Mb of Xq28 and formatted with nine previously reported probes and nine STSs developed from the sequence of probes and end fragments of YACs. YAC end fragments were obtained by subcloning, Alu-vector PCR, or primer-ligation PCR methods. Eightee...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1993.1234
更新日期:1993-06-01 00:00:00
abstract::We discuss the statistical significance of local similarities found between DNA sequences, and illustrate the procedure with reference to the Queen and Korn algorithm. If the longest similarity found for two sequences has length L, this length is said to be significant at the 5% level if there is a probability of no m...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(88)90081-x
更新日期:1988-10-01 00:00:00
abstract::The human genome harbors thousands of long terminal repeats (LTRs) that are derived from endogenous retroviruses and contain elements able to regulate the expression of neighboring cellular genes. We have investigated the ability of human endogenous retroviral (HERV)-K LTRs to provide transcriptional processing signal...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.2000.6175
更新日期:2000-05-15 00:00:00
abstract::The expression of plasma proteins changes dramatically as a result of cytokine induction, particularly interleukin-6, and their levels are used as clinical markers of inflammation. miRNAs are important regulators of gene expression and play significant roles in many inflammatory diseases and processes. The interaction...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2015.05.001
更新日期:2015-08-01 00:00:00
abstract::Current microarray technology allows researchers to genotype a large number of SNPs with relatively small amounts of DNA. Nevertheless, researchers and clinicians still frequently face the problem of acquiring enough high-quality DNA for analysis. Whole-genome amplification (WGA) methods offer a solution for this prob...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2008.08.007
更新日期:2008-12-01 00:00:00