Abstract:
:Interspersed repetitive sequence (IRS) PCR and repetitive element-to-bubble (IRS-bubble) PCR have been utilized to rapidly generate large numbers of mouse-specific, chromosome 1-enriched STSs from mouse-hamster somatic cell hybrids. Single-strand conformational polymorphism (SSCP) has been used to localize 39 new repetitive element-linked STSs to the mouse map: 22 to Chr 1, 10 to Chr. 15, 2 each to Chrs 12 and 14, and three to Chr 7. In addition, we have integrated the SSCP, single-strand length polymorphism, and restriction fragment length polymorphism maps of mouse Chr. 1, resulting in a high-density map of the chromosome, containing over 100 loci, all typed on a single interspecific backcross.
journal_name
Genomicsjournal_title
Genomicsauthors
Hunter KW,Watson ML,Rochelle J,Ontiveros S,Munroe D,Seldin MF,Housman DEdoi
10.1016/s0888-7543(11)80007-8subject
Has Abstractpub_date
1993-12-01 00:00:00pages
510-9issue
3eissn
0888-7543issn
1089-8646journal_volume
18pub_type
杂志文章相关文献
GENOMICS文献大全abstract::The human gene XPC (formerly designated XPCC), which corrects the repair deficiency of xeroderma pigmentosum (XP) group C cells, was mapped to 3p25. A cDNA probe for Southern blot hybridization and diagnostic PCR analyses of hybrid clone panels informative for human chromosomes in general and portions of chromosome 3 ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1994.1256
更新日期:1994-05-01 00:00:00
abstract::The human EWS gene encodes a putative RNA binding protein. As a result of acquired chromosome rearrangement, the N-terminal portion of the EWS protein is fused to the DNA binding domain of either FLI-1 or ERG in the Ewing family of tumors and to the DNA binding domain of ATF1 in malignant melanoma of soft parts. We ha...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1994.1495
更新日期:1994-09-01 00:00:00
abstract::The murine arylsulfatase A (ARSA) gene and cDNA have been cloned and sequenced. The gene is 3.8 kb long and contains eight exons. All intron/exon splice junctions conform to the GT/AG consensus sequence. The genomic structure is similar to that of the human gene. One major RNA species of 3.2 kb is transcribed. This RN...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1994.1055
更新日期:1994-01-15 00:00:00
abstract::We report the construction of a 1.5-Mb-resolution radiation hybrid map of the domestic cat genome. This new map includes novel microsatellite loci and markers derived from the 2X genome sequence that target previous gaps in the feline-human comparative map. Ninety-six percent of the 1793 cat markers we mapped have ide...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2006.08.007
更新日期:2007-02-01 00:00:00
abstract::The gene encoding activation-induced cytidine deaminase (AID), a member of the cytidine deaminase family, was isolated from a murine B cell lymphoma line, CH12F3-2, induced by combined stimulation of TGF-beta, IL-4, and CD40L. We have isolated the human orthologue of mouse AID cDNA, which has an open reading frame of ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.2000.6268
更新日期:2000-08-15 00:00:00
abstract::The human homeobox-containing genes EN1 and EN2 are closely related to the Drosophila pattern formation gene engrailed (en), which may be important in brain development, as shown by gene expression studies during mouse embryogenesis. Here, we have refined the localization of EN1 to human chromosome 2q13-q21 using a ma...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1993.1045
更新日期:1993-01-01 00:00:00
abstract::The frizzled (fz) locus in Drosophila is required for the transmission of polarity signals across the plasma membrane in epidermal cells, as well as to their neighboring cells in the developing wing. The identification of a tissue polarity gene from the fz locus in Drosophila melanogaster has been reported. The fz gen...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1995.1060
更新日期:1995-05-20 00:00:00
abstract::The E2F transcription factor plays an important regulatory role in cell proliferation, mediating the expression of genes whose products are essential for inducing resting cells to enter the cell cycle and synthesize DNA. To investigate the possible involvement of E2F in hematopoietic malignancies, we isolated genomic ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(95)80118-6
更新日期:1995-01-01 00:00:00
abstract::The v-ets oncogene family shares a conserved peptide motif called the ETS domain that mediates sequence-specific DNA binding. This motif is unique among transcription factor families. Using partially degenerate oligonucleotides from conserved regions of the ETS domain and the polymerase chain reaction, we isolated a n...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1994.1169
更新日期:1994-03-15 00:00:00
abstract::Activating mutations in the ras genes are commonly found in a wide range of human tumors. We recently cloned two mammalian genes, Son of sevenless 1 (mSos1) and Son of sevenless 2 (mSos2), whose protein products appear to be important positive regulators of ras proteins. Given the proposed role of Sos proteins in ras ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1993.1421
更新日期:1993-10-01 00:00:00
abstract::Southern blot analysis of genomic DNA from different strains of rat indicated that there were multiple copies of the gene encoding the second enzyme of the heme biosynthetic pathway, delta-aminolevulinate dehydratase (ALA-D). Two types of genomic clones were isolated from a Sprague-Dawley rat library. One appears to b...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(90)90210-l
更新日期:1990-08-01 00:00:00
abstract::A human clone corresponding to the gene for the DNA-binding factor LFB3, a protein highly homologous to the liver-specific transcription factor LFB1, has been isolated and partially sequenced. This gene is designated TCF2. Oligonucleotide primers have been designed for LFB3 and used to amplify specifically the human g...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(90)90239-q
更新日期:1990-09-01 00:00:00
abstract::Pulmonary surfactant consists of a complex mixture of phospholipids and several proteins essential to normal respiratory function. Two of the surfactant proteins, SP-A and SP-D, appear to have lectin-like activity relevant to the local phagocytic defense. Using polymerase chain reaction (PCR)-based somatic cell hybrid...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1993.1324
更新日期:1993-08-01 00:00:00
abstract::We have constructed a 1-Mb contig in human chromosomal band 11p15.5, a region implicated in the etiology of several embryonal tumors, including Wilms tumor, and in Beckwith-Wiedemann syndrome. Cosmid, P1, PAC, and BAC clones were characterized by NotI/SalI digestion and hybridized to a variety of probes to generate a ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1997.4826
更新日期:1997-08-01 00:00:00
abstract::Based on its map position, polymorphism pattern, and expression in the kidney, the gene encoding liver 20,000-30,000 MW protein 4 (LTW4) can be considered a potential candidate for the Jckm2 modifying locus, which mediates the severity of polycystic kidney disease in the juvenile cystic kidney mouse. Using two-dimensi...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1997.4762
更新日期:1997-06-15 00:00:00
abstract::The RBM (RNA-binding motif) gene family on the human Y chromosome encodes proteins with an RNA-binding domain. Its exclusive expression in germ cells and its partial deletion in some azoospermic or severely oligospermic males provide evidence of a role for RBM genes in spermatogenesis. There are approximately 30 RBM g...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1997.4944
更新日期:1997-10-15 00:00:00
abstract::Linkage of the gene responsible for an X-linked early onset parkinsonism disorder with mental retardation (McKusick 311510) to DNA probes that detect restriction fragment length polymorphisms is described. The disease gene is linked to the F8C gene, and to DNA probes detecting polymorphic loci DXS52, DXS15, DXS134, an...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(91)90363-j
更新日期:1991-04-01 00:00:00
abstract::Fragrance is an important component of end-use quality in rice. A set of 516 fragrant rice accessions were genotyped and over 80% of them carried the badh2.7 allele. A subset of 144 mostly fragrant accessions, including nine of Oryza rufipogon, was then subjected to a detailed diversity and haplotype analysis. The lev...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2012.11.010
更新日期:2013-02-01 00:00:00
abstract::The present investigation was focused to study genomic diversity of Indian swamp buffalo populations through reduced representation approach (ddRAD). The heterozygosity (FST) among the swamp buffaloes was 0.11 between Assam and Manipuri; 0.20 between swamp (Manipuri) and riverine buffaloes; 0.30 between swamp (Manipur...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2020.01.010
更新日期:2020-05-01 00:00:00
abstract::In line 4 transgenic mice, six to eight copies of a 50-kb lambda recombinant clone are arranged in a head-to-tail tandem array on chromosome 3. Embryos homozygous for the transgene become arrested in their development on Day 5 of gestation shortly after implantation. The insertion site was cloned using a small segment...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(92)90216-f
更新日期:1992-05-01 00:00:00
abstract::Desmoglein is a transmembrane glycoprotein of the cadherin superfamily present in the desmosomal junction in vertebrate epithelial cells. At least two variants of desmoglein are differentially expressed in human tissues: DGI, a characteristic desmosomal protein; and HDGC, which is, for example, expressed in the simple...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(92)90280-6
更新日期:1992-06-01 00:00:00
abstract::The gene for X-linked nephrogenic diabetes insipidus (NDI), a disorder which, if untreated, causes severe dehydration, mental retardation, and possibly death in affected males, has been mapped recently to the Xq28 band through demonstration of linkage to the DX552 locus and other DNA markers (N. Knoers et al., 1987, C...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(89)90352-2
更新日期:1989-04-01 00:00:00
abstract::Parasites of the genus Plasmodium infect a wide array of hosts, causing malaria in all major groups of terrestrial vertebrates including primates, reptiles, and birds. Molecular mechanisms explaining why some Plasmodium species are virulent, while other closely related malaria pathogens are relatively benign in the sa...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2018.12.004
更新日期:2019-12-01 00:00:00
abstract::Paulownia tomentosa is an important foundation forest tree species in semiarid areas. The lack of genetic information hinders research into the mechanisms involved in its response to abiotic stresses. Here, short-read sequencing technology (Illumina) was used to de novo assemble the transcriptome on P. tomentosa. A to...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2014.08.008
更新日期:2014-10-01 00:00:00
abstract::Among its known functions, tumor suppressor gene p53 serves as a transcriptional regulator and mediates various signals through activation of downstream genes. We recently identified a novel gene, GML (glycosylphosphatidylinositol (GPI)-anchored molecule-like protein), whose expression is specifically induced by wildt...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1997.4680
更新日期:1997-05-01 00:00:00
abstract::Bacterial artificial chromosomes (BACs) provide a well-characterized resource for studying the functional organization of genes and other large chromosomal domains. To facilitate functional studies in cell cultures, we have developed a simple approach for generating stable cell lines with variable copy numbers of any ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/s0888-7543(03)00205-2
更新日期:2004-01-01 00:00:00
abstract::Here, we report the complete genomic sequence and the characterization of the 311-kb region of 18q21, a candidate tumor suppressor locus containing a region of homozygous deletion in a lung cancer cell line, Ma29. This region contained two known genes, SMAD4 and ME2 (mitochondrial malate oxydoreductase), and two novel...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.2000.6454
更新日期:2001-03-01 00:00:00
abstract::Polymorphic loci that lie at the two extremities of the Duchenne/Becker muscular dystrophy (DMD/BMD) gene have been used to estimate intragenic recombination rates. Multipoint linkage analysis of the CEPH panel of families suggests a total intragenic recombination frequency of nearly 0.12 (confidence intervals 0.041-0...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(90)90205-9
更新日期:1990-08-01 00:00:00
abstract::A wide range of autoimmune and other diseases are known to be associated with the major histocompatibility complex. Many of these diseases are linked to the genes encoding the polymorphic histocompatibility antigens in the class I and class II regions, but some appear to be more strongly associated with genes in the c...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1996.0459
更新日期:1996-09-01 00:00:00
abstract::In Macaca mulatta, the single rDNA array is flanked by a patchwork of sequences including subregions of human Yp11.2, 4q35.2, and 10p15.3. This composite DNA region is characterized by unique or low-copy sequences, resembling a potentially transcribed region. The analysis of Cercopithecus aethiops, Presbytis cristata,...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2006.05.001
更新日期:2006-11-01 00:00:00