Abstract:
:Bacterial artificial chromosomes (BACs) provide a well-characterized resource for studying the functional organization of genes and other large chromosomal domains. To facilitate functional studies in cell cultures, we have developed a simple approach for generating stable cell lines with variable copy numbers of any BAC. Here we describe hamster cell lines with BAC transgenes that express mouse Cdc6 at levels that correlate with BAC copy number; show that mouse Cdc6 is regulated normally during the cell cycle, binds chromatin, and is degraded during apoptosis; and report a novel fraction of Cdc6 that associates with the spindle apparatus during mitosis. With RNA interference to assess genetic complementation by BAC alleles, this system will facilitate functional studies on large chromosomal domains at variable copy number in mammalian cell models.
journal_name
Genomicsjournal_title
Genomicsauthors
Illenye S,Heintz NHdoi
10.1016/s0888-7543(03)00205-2subject
Has Abstractpub_date
2004-01-01 00:00:00pages
66-75issue
1eissn
0888-7543issn
1089-8646pii
S0888754303002052journal_volume
83pub_type
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