Abstract:
:DNA libraries often contain very large numbers of clones (from 1000 up to 700,000). Since at present it is impossible to analyze all of these clones, usually statistical samples comprising less than 100 clones are tested. The quality of the library is then assessed by linear extrapolation. Occasionally, full coverage of chromosomal regions by DNA probes is inferred from this. However, this may not be accurate since linear extrapolation is misleading and the statistical samples are generally too small to characterize the libraries. A quantitative model of the distribution of the frequencies of the clones in a library is mandatory for any useful assessment of the quality of the library. Otherwise, it is very difficult to draw useful conclusions from moderately sized samples. Examples from everyday life and formulas are given to determine the quality of a library and useful sample sizes.
journal_name
Genomicsjournal_title
Genomicsauthors
Köllner M,Greulich KOdoi
10.1006/geno.1994.1476subject
Has Abstractpub_date
1994-09-01 00:00:00pages
185-91issue
1eissn
0888-7543issn
1089-8646pii
S0888-7543(84)71476-5journal_volume
23pub_type
杂志文章相关文献
GENOMICS文献大全abstract::The majority of Williams-Beuren syndrome (WBS) patients have been shown to have a microdeletion within 7q11.2 including the elastin gene locus. The extent of these deletions has, however, not been well characterized. Thirty-five deletion patients were tested for all polymorphic markers in the 7q11.2 region bounding EL...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1996.0237
更新日期:1996-05-15 00:00:00
abstract::Autosomal dominant hereditary spastic paraplegia (AD-HSP) is a genetically heterogeneous disorder characterized by progressive spasticity of the lower limbs. A major locus (SPG4) causing AD-HSP in about 40% of the families was mapped to chromosome 2p. The analysis of six SPG4-linked AD-HSP families using the RED proce...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1999.5932
更新日期:1999-09-15 00:00:00
abstract::Identification of all the transcription factors (TFs) encoded in a given genome is a prerequisite for understanding transcriptional regulatory networks. Archaea are prokaryotes that constitute one of the three main branches of organisms with an astounding diversity of habitats. In this report, we establish the Archaea...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2007.09.007
更新日期:2008-01-01 00:00:00
abstract::We conducted a comprehensive analysis of codon usage bias (CUB) based on the available non-redundant full-length cDNA (nrFLcDNA) and expressed sequence tags (ESTs) data of cultivar Micro-Tom and evaluated the associations of observed CUB and measurements of transcriptional and translational effectiveness. The analysis...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2013.02.008
更新日期:2013-05-01 00:00:00
abstract::Development of statistical methods has become very necessary for large-scale correlation analysis in the current "omic" data. We propose ranking analysis of correlation coefficients (RAC) based on transforming correlation matrix into correlation vector and conducting a "locally ranking" strategy that significantly red...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2010.09.002
更新日期:2011-01-01 00:00:00
abstract::The genetic linkage of Chediak-Higashi syndrome and its murine analog, beige (bg), to the T-cell receptor (TCR-gamma) gamma chain gene is further defined. Previous studies using recombinant inbred strains of mice demonstrated that the murine bg gene is genetically linked to a murine TCR-gamma gene. We report that in t...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(87)90058-9
更新日期:1987-11-01 00:00:00
abstract::We report the cDNA sequence for the bovine gene for fibrillin corresponding to the human gene, fibrillin 1 (FBN1), and the localization of the gene to bovine chromosome 10 (syntenic group U5). The identity between the human and bovine sequences is 97.8% at the amino acid level and 92% at the nucleotide level. The bovi...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1994.1527
更新日期:1994-09-15 00:00:00
abstract::While scanning for single-nucleotide polymorphisms (SNPs) in the human Xq25-q28 region of CEPH families, we found six long "deserts" of low SNP incidence representing 28% of the investigated genome. One was 1.66 Mb in length. To determine whether these SNP deserts were due to reduced input of mutations or to recent co...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.2000.6417
更新日期:2001-01-01 00:00:00
abstract::Head and neck squamous cell carcinoma (HNSCC) is a malignant tumor of the upper aerodigestive tract. The loss and gain of miRNA function promote cancer development through various mechanisms. RNA sequencing (RNA-seq) and miRNAs sequencing data from the Cancer Genome Atlas (TCGA) was used to show the dysfunctional miRN...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2020.12.002
更新日期:2021-01-01 00:00:00
abstract::We report a comparative map of canine chromosome 1 (CFA1) incorporating single nucleotide polymorphisms (SNPs) and insertion/deletion (indel) polymorphisms, developed by using cross-species primers, radiation hybrid analysis, and pool-and-sequence identification of genetic variations. Fifty-five genes were chosen with...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2004.04.001
更新日期:2004-08-01 00:00:00
abstract::We have previously reported that the expression of HC gp-39, a 39-kDa secretory glycoprotein and member of the chitinase protein family, is associated with late stages of monocyte to macrophage maturation. To allow further investigations of its unique expression pattern and to facilitate studies on the regulation of t...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1997.4778
更新日期:1997-07-15 00:00:00
abstract::Complete or partial monosomy 7 is a recurring cytogenetic abnormality in acute myelogenous leukemia (AML) and myeloproliferative syndromes (MPS) and is particularly common in patients with Fanconi's anemia and in secondary AML. A familial form of monosomy 7 has been recognized in which two or more siblings develop MPS...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/s0888-7543(05)80293-9
更新日期:1992-09-01 00:00:00
abstract::The APETALA2/ethylene-responsive factor (AP2/ERF) has important roles in regulating developmental processes and hormone signaling transduction in plants. Pineapple demonstrates a special sensitivity to ethylene, and AP2/ERFs may contribute to this distinct sensitivity of pineapples to ethylene. However, little informa...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2020.10.040
更新日期:2021-01-20 00:00:00
abstract::Single-pass sequencing of randomly selected cDNA clones to generate expressed sequence tags (ESTs) has been widely used to identify novel genes and to study gene expression in a variety of tissues. We have generated 2244 ESTs from a human fetal heart library (GenBank Accession Nos. R30692-30774 and R56965-58824), whic...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1995.9874
更新日期:1995-11-20 00:00:00
abstract::A previous in situ hybridization study with a Pi class glutathione S-transferase cDNA probe revealed the presence of hybridizing sequences on the long arms of chromosomes 11 and 12. Since the GSTP1 gene is known to be on chromosome 11 and since it is thought that chromosomes 11 and 12 arose from an ancient tetraploidi...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/s0888-7543(05)80243-5
更新日期:1992-10-01 00:00:00
abstract::The identification of noncoding functional elements within vertebrate genomes, such as those that regulate gene expression, is a major challenge. Comparisons of orthologous sequences from multiple species are effective at detecting highly conserved regions and can reveal potential regulatory sequences. The GDF6 gene c...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2005.05.003
更新日期:2005-09-01 00:00:00
abstract::The human homeobox gene HLX1 appears to be involved in hemopoietic development and may represent a candidate gene for various developmental or hemopoietic disorders. We have isolated genomic clones for the gene, determined its intron-exon organization, and confirmed its map location on chromosome 1q41-q42. The transcr...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1994.1394
更新日期:1994-07-15 00:00:00
abstract::A comparison study of short SAGE versus GeneChip and long SAGE was conducted to determine if data were interchangeable between the techniques. Although SAGE and Affymetrix chip expression levels showed a significant correlation using the set of genes for which there was reliable and unambiguous mapping from tag-to-gen...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2004.06.014
更新日期:2004-10-01 00:00:00
abstract::Polymorphic loci that lie at the two extremities of the Duchenne/Becker muscular dystrophy (DMD/BMD) gene have been used to estimate intragenic recombination rates. Multipoint linkage analysis of the CEPH panel of families suggests a total intragenic recombination frequency of nearly 0.12 (confidence intervals 0.041-0...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(90)90205-9
更新日期:1990-08-01 00:00:00
abstract::The canine species, including wolf and jackal, have four digits on the hind limb. It was thought that an extra first digit on the hind limb, named dewclaw, was a hereditary defect. For genetically related canine pedigrees with 73 members with dewclaws, we carried out a genome-wide scan for linkage with microsatellites...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/s0888-7543(03)00234-9
更新日期:2004-02-01 00:00:00
abstract::Several neuropsychiatric disorders map to human 15q13-q14, which contains a strong candidate in the alpha7-nicotinic acetylcholine receptor subunit gene (CHRNA7) and is partly duplicated, complicating further genetic analysis. We have shown that the partial duplication is in a hybrid (CHRFAM7A)between CHRNA7 and one o...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.2002.6694
更新日期:2002-02-01 00:00:00
abstract::Aneuploidy is a characteristic of the majority of human cancers, and recent work has suggested that mitotic checkpoint defects play a role in its development. To further explore this issue, we isolated a novel human gene, MAD2B (MAD2L2), which is homologous to the spindle checkpoint gene MAD2 (MAD2L1). We determined t...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1999.5831
更新日期:1999-06-01 00:00:00
abstract::In response to nutrient deprivation, the ubiquitous Gram-negative soil bacterium Myxococcus xanthus undergoes a well-characterized developmental response, resulting in the formation of a multicellular fruiting body. The center of the fruiting body consists of myxospores; surrounding this structure are rod-shaped perip...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2019.09.008
更新日期:2020-03-01 00:00:00
abstract::We have characterized three terminal deletions of the long arm of the X chromosome. Southern analysis using Xq27/q28 probes suggests that two of the deletions have breakpoints near the fragile site at Xq27.3. Flow karyotype analysis provides an estimate of 12 X 10(6) bp for the size of the deleted region. We have not ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(87)90028-0
更新日期:1987-12-01 00:00:00
abstract::A wide range of autoimmune and other diseases are known to be associated with the major histocompatibility complex. Many of these diseases are linked to the genes encoding the polymorphic histocompatibility antigens in the class I and class II regions, but some appear to be more strongly associated with genes in the c...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1996.0459
更新日期:1996-09-01 00:00:00
abstract::Using an interspecies backcross, we have mapped the HOX-5 and surfeit (surf) gene clusters within the proximal portion of mouse chromosome 2. While the HOX-5 cluster of homeobox-containing genes has been localized to chromosome 2, bands C3-E1, by in situ hybridization, its more precise position relative to the genes a...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(90)90499-k
更新日期:1990-04-01 00:00:00
abstract::We have mapped 13 loci on mouse Chromosome 18 by Southern blot analysis of restriction fragment length polymorphisms among progeny from an interspecific backcross: (C57BL/6J X Mus spretus) X M. spretus. Complete haplotype analysis of 136 of these progeny was used to establish gene order and estimate genetic distances ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(92)90029-r
更新日期:1992-08-01 00:00:00
abstract::Glaucoma is a very common disorder of the eye wherein the disturbance of the structural or functional integrity of the optic nerve causes characteristic atrophic changes in the optic nerve, which may lead to specific visual field defects over time. Primary open angle glaucoma (POAG) is most frequent among the three pr...
journal_title:Genomics
pub_type: 杂志文章,评审
doi:10.1016/j.ygeno.2016.11.003
更新日期:2017-01-01 00:00:00
abstract::Cri-du-chat is a chromosomal deletion syndrome characterized by partial deletion of the short arm of chromosome 5. The clinical symptoms include growth and mental retardation, microcephaly, hypertelorism, epicanthal folds, hypotonia, and a high-pitched monochromatic cry that is usually considered diagnostic for the sy...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1994.1582
更新日期:1994-11-01 00:00:00
abstract::We describe an improved method for construction of yeast artificial-chromosome (YAC) libraries that contain large inserts of foreign DNA. The procedure consists of seven steps: (i) preparation of human DNA in agarose beads; (ii) partial digestion of the DNA with EcoRI; (iii) electrophoretic elimination of the smaller ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(90)90285-3
更新日期:1990-10-01 00:00:00