Abstract:
:We developed a model of influenza virus infection of neutrophils by inducing differentiation of the MPRO promyelocytic cell line. After 5 days of differentiation, about 20-30% of mature neutrophils could be detected. Only a fraction of neutrophils were infected by highly virulent influenza (HVI) virus, but were unable to support active viral replication compared with MDCK cells. HVI infection of neutrophils augmented early and late apoptosis as indicated by annexin V and TUNEL assays. Comparison between the global transcriptomic responses of neutrophils to HVI and low virulent influenza (LVI) revealed that the IFN regulatory factor and IFN signaling pathways were the most significantly overrepresented pathways, with activation of related genes in HVI as early as 3 h. Relatively consistent results were obtained by real-time RT-PCR of selected genes associated with the type I IFN pathway. Early after HVI infection, comparatively enhanced expression of apoptosis-related genes was also elicited.
journal_name
Genomicsjournal_title
Genomicsauthors
Ivan FX,Tan KS,Phoon MC,Engelward BP,Welsch RE,Rajapakse JC,Chow VTdoi
10.1016/j.ygeno.2012.11.008subject
Has Abstractpub_date
2013-02-01 00:00:00pages
101-12issue
2eissn
0888-7543issn
1089-8646pii
S0888-7543(12)00228-5journal_volume
101pub_type
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