The two-component signal system in rice (Oryza sativa L.): a genome-wide study of cytokinin signal perception and transduction.

Abstract:

:In this report we define the genes of two-component regulatory systems in rice through a comprehensive computational analysis of rice (Oryza sativa L.) genome sequence databases. Thirty-seven genes were identified, including 5 HKs (cytokinin-response histidine protein kinase) (OsHK1-4, OsHKL1), 5 HPs (histidine phosphotransfer proteins) (OsHP1-5), 15 type-A RRs (response regulators) (OsRR1-15), 7 type B RR genes (OsRR16-22), and 5 predicted pseudo-response regulators (OsPRR1-5). Protein motif organization, gene structure, phylogenetic analysis, chromosomal location, and comparative analysis between rice, maize, and Arabidopsis are described. Full-length cDNA clones of each gene were isolated from rice. Heterologous expression of each of the OsHKs in yeast mutants conferred histidine kinase function in a cytokinin-dependent manner. Nonconserved regions of individual cDNAs were used as probes in expression profiling experiments. This work provides a foundation for future functional dissection of the rice cytokinin two-component signaling pathway.

journal_name

Genomics

journal_title

Genomics

authors

Du L,Jiao F,Chu J,Jin G,Chen M,Wu P

doi

10.1016/j.ygeno.2007.02.001

subject

Has Abstract

pub_date

2007-06-01 00:00:00

pages

697-707

issue

6

eissn

0888-7543

issn

1089-8646

pii

S0888-7543(07)00049-3

journal_volume

89

pub_type

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