Abstract:
:Proprotein convertase 4 (PC4) is a mammalian secretory serine endoproteinase similar to the yeast KEX2 gene product and specifically expressed in testicular germs cells. PC4 mRNA isoforms that vary in size and 3' coding sequence have been reported (N. G. Seitah, R. Day, J. Hamelin, A. Gaspar, M. W. Collard, and M. Chrétien, 1992, Mol. Endocrinol. 6: 1559-1570). To determine the origin of these various forms, the mouse PC4 gene was cloned and its organization determined. The structural gene is approximately 9.5 kb long. It contains 15 exons and 14 introns. The exon-intron organization is very similar to that of the genes for the related convertases furin, PC1, and PC2. The upstream region carries several GGGCGG and three CCAAT but no TATAA motifs. Analysis of the 5' end of PC4 mRNA in the testis has led to the identification of two novel 5' splice variants that might encode a nonsecretory enzyme. The multiple forms of PC4 mRNA can all be explained by alternate splicing of primary transcripts of a single gene.
journal_name
Genomicsjournal_title
Genomicsauthors
Mbikay M,Raffin-Sanson ML,Tadros H,Sirois F,Seidah NG,Chretien Mdoi
10.1006/geno.1994.1158subject
Has Abstractpub_date
1994-03-15 00:00:00pages
231-7issue
2eissn
0888-7543issn
1089-8646pii
S0888-7543(84)71158-Xjournal_volume
20pub_type
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