Abstract:
:Fluorescence in situ hybridization mapping of fully integrated human BAC clones to primate chromosomes, combined with precise breakpoint localization by PCR analysis of flow-sorted chromosomes, was used to analyze the evolutionary rearrangements of the human 3q21.3-syntenic region in orangutan, siamang gibbon, and silvered-leaf monkey. Three independent evolutionary breakpoints were localized within a 230-kb segment contained in BACs RP11-93K22 and RP11-77P16. Approximately 200 kb of the human 3q21.3 sequence was not present on the homologous orangutan, siamang, and Old World monkey chromosomes, suggesting a genomic DNA insertion into the breakpoint region in the lineage leading to humans and African great apes. The breakpoints in the orangutan and siamang genomes were narrowed down to 12- and 20-kb DNA segments, respectively, which are enriched with endogenous retrovirus long terminal repeats and other repetitive elements. The inserted DNA segment represents part of an ancestral duplication. Paralogous sequence blocks were found at human 3q21, approximately 4 Mb proximal to the evolutionary breakpoint cluster region; at human 3p12.3, which contains an independent orangutan-specific breakpoint; and at the subtelomeric and pericentromeric regions of multiple human and orangutan chromosomes. The evolutionary breakpoint regions between human chromosome 3 and orangutan 2 as well their paralogous segments in the human genome coincide with breaks of chromosomal synteny in the mouse, rat, and/or chicken genomes. Collectively our data reveal reuse of the same short recombinogenic DNA segments in primate and vertebrate evolution, supporting a nonrandom breakage model of genome evolution.
journal_name
Genomicsjournal_title
Genomicsauthors
Yue Y,Grossmann B,Ferguson-Smith M,Yang F,Haaf Tdoi
10.1016/j.ygeno.2004.10.007subject
Has Abstractpub_date
2005-01-01 00:00:00pages
36-47issue
1eissn
0888-7543issn
1089-8646pii
S0888-7543(04)00281-2journal_volume
85pub_type
杂志文章相关文献
GENOMICS文献大全abstract::The cytotoxic activity of mouse natural killer cells is regulated in part through cell surface molecules belonging to the Ly49 multigene family. In mice, the genomic sequence of the Ly49 gene cluster has been examined in detail and this analysis provided a model of the expansion of this multigene family. In the presen...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2004.01.010
更新日期:2004-07-01 00:00:00
abstract::Talin is a high-molecular-weight cytoskeletal protein, localized at cell-extracellular matrix associations known as focal contacts. In these regions, talin is thought to link integrin receptors to the actin cytoskeleton. Talin plays a key role in the assembly of actin filaments and in spreading and migration of variou...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1999.6019
更新日期:1999-12-01 00:00:00
abstract::The genomic structure of the mouse gene encoding the CDEBP protein has been established. The protein was initially identified on the basis of its ability to bind the CDEI motif (GTCACATG). The same locus has been independently described under the name APLP2, on the basis of sequence similarities with the Amyloid Precu...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1996.0318
更新日期:1996-07-01 00:00:00
abstract::FISH techniques have opened new possibilities for high-resolution genome mapping. Effective utilization of these techniques for the rapid orientation and ordering of adjacent and overlapping probes as well as for the characterization of long-range genomic contigs would facilitate physical mapping and positional clonin...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1995.0005
更新日期:1995-11-01 00:00:00
abstract::A new Alcanivorax sp. VBW004 was isolated from a shallow hydrothermal vent in Azores Island, Portugal. In this study, we determined VBW004 was resistant to copper. This strain showed maximum tolerance of copper concentrations up to 600 μg/mL. Based on 16S rRNA gene sequencing and phylogeny revealed that this strain wa...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2020.06.015
更新日期:2020-09-01 00:00:00
abstract::Chromosomal region 1p36.2 harbors an intriguing gene cluster of about 1 Mb. In addition to normal high-copy-number repeats, this cluster consists entirely of locally repeated sequences among which there are tRNA and small nuclear RNA (snRNA) genes. In 23 PACs and YACs from the 1p36.2 cluster, we identified eight diffe...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1999.5972
更新日期:1999-11-15 00:00:00
abstract::The region of the dystrophin gene containing introns 45-50 is characterized by a high rate of recombination events that give rise to large deletions causing dystrophinopathy. The nucleotide sequence of this intronic region has recently been released in GenBank. With the aim of further understanding the mechanism favor...
journal_title:Genomics
pub_type: 杂志文章
doi:
更新日期:2002-11-01 00:00:00
abstract::To investigate the molecular mechanism of silkworm resistance to BmNPV infection, we constructed a near-isogenic line (BC8) with BmNPV resistance using highly resistant (NB) and highly susceptible parental strains (306). We investigated variations in the gene expression in the midguts of BmNPV-infected BC8 and 306 at ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2013.02.004
更新日期:2013-04-01 00:00:00
abstract::The Dlk1-Dio3 imprinted domain on mouse chromosome 12 contains IG-DMR and Gtl2-DMR, whose methylation patterns are established in the germline and after fertilization, respectively. In this study, we determine that acquisition of DNA methylation at the paternal allele of the Gtl2-DMR is initiated after the blastocyst ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2011.05.003
更新日期:2011-08-01 00:00:00
abstract::Knowledge of protein subcellular localization is vitally important for both basic research and drug development. With the avalanche of protein sequences emerging in the post-genomic age, it is highly desired to develop computational tools for timely and effectively identifying their subcellular localization purely bas...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2018.05.017
更新日期:2019-07-01 00:00:00
abstract::A novel method for identifying DNA point mutations has been developed by using mismatch repair enzymes. The high specificity of the Escherichia coli MutY protein has permitted the development of a reliable and sensitive method for the detection and characterization of point mutations in the human genome. The MutY prot...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/s0888-7543(05)80213-7
更新日期:1992-10-01 00:00:00
abstract::We have mapped the mouse protein tyrosine phosphatase epsilon (PTP epsilon, gene symbol Ptpre) gene to the distal region of chromosome 7 by linkage analysis using two sets of multilocus genetic crosses. The human PTP epsilon gene (gene symbol PTPRE) was mapped to chromosome 10q26 by fluorescence in situ hybridization....
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1996.0061
更新日期:1996-02-01 00:00:00
abstract::We investigated a family with a duplication, dup(X)q26-q27, that was present in two brothers, their mother, and their maternal grandmother. The brothers carrying the duplication displayed spina bifida and panhypopituitarism, whereas a third healthy brother inherited the normal X chromosome. Preferential inactivation o...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.2000.6327
更新日期:2000-10-15 00:00:00
abstract::Cytogenetic studies of banded chromosomes and fluorescence in situ hybridization (FISH) of several yeast artificial chromosomes (YACs) that are part of a 128-kb resolution physical map of a portion of 12q13 revealed that 4/14 (28%) lipomas have breakpoints in 12q13. These breakpoints are more than 10 Mb away from the ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1997.4993
更新日期:1997-11-15 00:00:00
abstract::The hemopoietic growth factors interleukin-3 (IL-3, multi-CSF) and granulocyte-macrophage colony-stimulating factor (GM-CSF) belong to a family of secreted glycoproteins that stimulate the proliferation and differentiation of hemopoietic progenitor cells. IL-3 and GM-CSF have overlapping biological activities and show...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(89)90070-0
更新日期:1989-08-01 00:00:00
abstract::To understand the pattern of gene expression in mouse myeloid progenitor cells, we carried out a genome-wide analysis of gene expression in mouse bone marrow Gr-1(+) cells using SAGE and GLGI techniques. We identified 22,033 unique SAGE tags with quantitative information from 73,869 collected SAGE tags. Among these un...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.2001.6633
更新日期:2001-10-01 00:00:00
abstract::The lack of physical mapping data strongly restricts the analysis of the meningioma chromosomal region that was assigned to the bands 22q12.3-qter. Recently, we reported a new marker D22S16 for chromosome 22 that was assigned to the region 22q13-qter by in situ hybridization. Utilizing somatic cell hybrids we now subl...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(91)90196-l
更新日期:1991-08-01 00:00:00
abstract::Changing bovine milk fatty acid (FA) composition through selection can decrease saturated FA (SFA) consumption, improve human health and provide a means for manipulating processing properties of milk. Our study determined associations between milk FA composition and genes from triacylglycerol (TAG) biosynthesis pathwa...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2014.10.001
更新日期:2014-12-01 00:00:00
abstract::MicroRNAs control gene expression at the posttranscriptional level by base-pairing to the 3'-UTR of their target mRNAs, thus leading to mRNA degradation of protein fabrication. We hypothesize, SNPs within miRNAs and their targets could be of significance to an individual's risk of developing cancer. We analyzed in sil...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2011.06.005
更新日期:2011-09-01 00:00:00
abstract::Using an interspecies backcross, we have mapped the HOX-5 and surfeit (surf) gene clusters within the proximal portion of mouse chromosome 2. While the HOX-5 cluster of homeobox-containing genes has been localized to chromosome 2, bands C3-E1, by in situ hybridization, its more precise position relative to the genes a...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(90)90499-k
更新日期:1990-04-01 00:00:00
abstract::Protein sequences were compared among Arabidopsis, Oryza and Populus to identify differential gene (DG) sets that are in one but not the other two genomes. The DG sets were screened against a plant transcript database, the NR protein database and six newly-sequenced genomes (Carica, Glycine, Medicago, Sorghum, Vitis a...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2009.01.002
更新日期:2009-05-01 00:00:00
abstract::Isozyme and restriction fragment length polymorphism (RFLP) analyses of backcross progeny, recombinant inbred strains, and congenic strains of mice positioned eight genetic markers with respect to the Lsh-Ity-Bcg disease resistance locus. Allelic isoforms of Idh-1 and Pep-3 and RFLPs detected by Southern hybridization...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(90)90518-y
更新日期:1990-05-01 00:00:00
abstract::Lymphocytes of mouse strains BALB/cHeA (BALB/c) and STS/A (STS) differ in the IL-2-induced proliferative response, STS being a high and BALB/c a low responder in the range of concentrations 125-2000 IE/ml. We analyzed the genetic basis of this strain difference using the recombinant congenic (RC) strains of the BALB/c...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1997.4694
更新日期:1997-05-15 00:00:00
abstract::Single nucleotide polymorphism (SNP) interactions can explain the missing heritability of common complex diseases. Many interaction detection methods have been proposed in genome-wide association studies, and they can be divided into two types: population-based and family-based. Compared with population-based methods,...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2018.07.014
更新日期:2019-09-01 00:00:00
abstract::The aim of this study was to elucidate the roles played by circular RNAs (circRNAs) in the mechanism underlying submandibular gland (SMG) dysfunction in hypertension. We employed RNA-seq to analyze the circRNA and mRNA expression profiles of SMGs. Seventy-five differentially expressed (DE) circRNAs and 691 DE mRNAs we...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2020.11.016
更新日期:2021-01-01 00:00:00
abstract::Prostaglandin EP3 receptor subtype is a seven-membrane-spanning protein with multiple C-terminal tails generated by alternative mRNA splicing. We report here the structural organization of the human EP3 gene (PTGER3). The human EP3 gene spanned more than 80 kb and was composed of 10 exons separated by nine introns. Ex...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1996.4585
更新日期:1997-03-15 00:00:00
abstract::Salinity is a major limiting factor in crop production. Exogenous spermidine (spd) effectively ameliorates salt injury, though the underlying molecular mechanism is poorly understood. We have used a suppression subtractive hybridization method to construct a cDNA library that has identified up-regulated genes from ric...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2020.07.011
更新日期:2020-11-01 00:00:00
abstract::The allele-specific polymerase chain reaction (ASPCR) procedure has proven a powerful tool for the detection and analysis of known genetic polymorphisms. Here, we present a novel application of the ASPCR technique to determine the ABO genotypes of individuals without the need of family analysis. The method introduces ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(92)90292-z
更新日期:1992-04-01 00:00:00
abstract::Chromosomal locations have been assigned to seven members of the TGF-beta superfamily using an interspecific mouse backcross. Probes for the Tgfb-1, -2, and -3, Bmp-2a and -3, and Vgr-1 genes recognized only single loci, whereas the Bmp-2b probe recognized two independently segregating loci (designated Bmp-2b1 and Bmp...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(90)90480-i
更新日期:1990-03-01 00:00:00
abstract::We used targeted exon trapping to clone portions of genes from human chromosome 21q22.3. One trapped sequence showed complete homology with the cDNA of human U2AF35 (M96982; HGM-approved nomenclature U2AF1), which encodes for the small 35-kDa subunit of the U2 snRNP auxiliary factor. Using the U2AF1 cDNA as a probe, w...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1996.0196
更新日期:1996-04-15 00:00:00