Abstract:
:The Dlk1-Dio3 imprinted domain on mouse chromosome 12 contains IG-DMR and Gtl2-DMR, whose methylation patterns are established in the germline and after fertilization, respectively. In this study, we determine that acquisition of DNA methylation at the paternal allele of the Gtl2-DMR is initiated after the blastocyst stage and completed by embryonic day 6.5, and that Gtl2 (approved symbol: Meg3) is monoallelically expressed from the maternal allele as early as the blastocyst. Therefore, DNA methylation at the Gtl2-DMR is not a prerequisite for the imprinted expression of Gtl2, which may be involved in the control of proliferation and differentiation of cells during early gestation. We also reveal that a subregion of the IG-DMR exhibits tissue-specific differences in allelic methylation patterns. These results add to the growing body of knowledge elucidating the mechanism whereby parent-of-origin-dependent DNA methylation at the IG-DMR leads to the imprinted expression of the Dlk1-Dio3 cluster.
journal_name
Genomicsjournal_title
Genomicsauthors
Sato S,Yoshida W,Soejima H,Nakabayashi K,Hata Kdoi
10.1016/j.ygeno.2011.05.003subject
Has Abstractpub_date
2011-08-01 00:00:00pages
120-7issue
2eissn
0888-7543issn
1089-8646pii
S0888-7543(11)00127-3journal_volume
98pub_type
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