Abstract:
:Salinity is a major limiting factor in crop production. Exogenous spermidine (spd) effectively ameliorates salt injury, though the underlying molecular mechanism is poorly understood. We have used a suppression subtractive hybridization method to construct a cDNA library that has identified up-regulated genes from rice root under the treatment of spd and salt. Total 175 high-quality ESTs of about 100-500 bp in length with an average size of 200 bp are isolated, clustered and assembled into a collection of 62 unigenes. Gene ontology analysis using the KEGG pathway annotation database has classified the unigenes into 5 main functional categories and 13 subcategories. The transcripts abundance has been validated using Real-Time PCR. We have observed seven different types of post-translational modifications in the DEPs. 44 transmembrane helixes are predicted in 6 DEPs. This above information can be used as first-hand data for dissecting the administrative role of spd during salinity.
journal_name
Genomicsjournal_title
Genomicsauthors
Saha J,Giri K,Roy Sdoi
10.1016/j.ygeno.2020.07.011subject
Has Abstractpub_date
2020-11-01 00:00:00pages
4125-4136issue
6eissn
0888-7543issn
1089-8646pii
S0888-7543(19)30031-Xjournal_volume
112pub_type
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