Abstract:
:We have isolated cDNA clones for the gene, termed GPX1, encoding the major human selenoprotein, glutathione peroxidase. Sequence analysis confirmed previous findings that the unusual amino acid seleno-cysteine is encoded by the opal terminator codon UGA. Southern blot analysis of human genomic DNA with the GPX1 cDNA showed that restriction endonucleases without sites in the probe sequence produced three hybridizing bands at standard stringency, diminishing to one strongly and one weakly hybridizing band at high stringency. In situ hybridization localized the human GPX1 gene to a single site on chromosome 3, at region 3q11-13.1. Thus, three genomic sites bear sequence homology to the GPX1 cDNA, and the one most homologous maps to 3q11-13.1.
journal_name
Genomicsjournal_title
Genomicsauthors
Chada S,Le Beau MM,Casey L,Newburger PEdoi
10.1016/0888-7543(90)90566-dsubject
Has Abstractpub_date
1990-02-01 00:00:00pages
268-71issue
2eissn
0888-7543issn
1089-8646pii
0888-7543(90)90566-Djournal_volume
6pub_type
杂志文章相关文献
GENOMICS文献大全abstract::Low-molecular-weight, calcium-dependent phospholipase A2 genes (PLA2s) that belong to the secreted type of PLA2s are clustered within a syntenic group on human 1p35-p36 and mouse 4qD3. We reassembled trace files available from the Whole Genome Sequencing (WGS) Project, obtaining an 86-kb contig with three tandem PLA2G...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2005.12.015
更新日期:2006-04-01 00:00:00
abstract::We have analyzed tumor and lymphocyte DNA from six breast cancer patients by one- and two-dimensional DNA fingerprinting using micro- and minisatellite core probes to estimate the extent and nature of DNA alterations in tumors. Both approaches were compared regarding sensitivity in genome analysis. We find that the nu...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1993.1284
更新日期:1993-07-01 00:00:00
abstract::A novel method for identifying DNA point mutations has been developed by using mismatch repair enzymes. The high specificity of the Escherichia coli MutY protein has permitted the development of a reliable and sensitive method for the detection and characterization of point mutations in the human genome. The MutY prot...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/s0888-7543(05)80213-7
更新日期:1992-10-01 00:00:00
abstract::Proteins with seven transmembrane segments (7TM) define a superfamily of receptors (7TM receptors) sharing the same topology: an extracellular N-terminus, three extramembranous loops on either side of the plasma membrane, and a cytoplasmic C-terminal tail. Upon ligand binding, cytoplasmic portions of the activated rec...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(95)80218-b
更新日期:1995-03-20 00:00:00
abstract::Sp4H is a semi-dominant mutation that maps to mouse chromosome 1. Heterozygous mice exhibit white spotting of the belly, whereas the fate of the homozygous embryos is unknown. We have previously shown that the entire coding region of the Pax3 gene is deleted in the Sp4H mutant. In this study, we have analyzed the fate...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1996.0267
更新日期:1996-06-01 00:00:00
abstract::Na(+)-coupled HCO(3)(-) transporters (NCBTs) of the SLC4 family play critical roles in pH regulation as well as transepithelial HCO(3)(-) transport. We systematically examined, in the mouse reproductive tract tissues, the mRNA expression of five NCBTs as well as the five NBCe1 (Slc4a4) variants NBCe1-A through -E, of ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2011.04.010
更新日期:2011-08-01 00:00:00
abstract::Physical maps are important resources both in sequencing and in functional analyses of large genomes. Global contig-building approaches are regarded to be more efficient relative to the cumulative outcome of scattered and more localized physical mapping studies accompanying positional cloning. This work is part of an ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1999.5973
更新日期:1999-11-15 00:00:00
abstract::The AZ1 protein is localized to the preacrosome region of spermatids. Previous developmental studies suggested that AZ1 gene transcription begins in pachytene spermatocytes and that expression of the gene is induced in cultivated fibroblasts on treatment with 5-azacytidine, which is known to lead to the demethylation ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1996.4546
更新日期:1997-02-15 00:00:00
abstract::The LIM domain is a structural motif that is well conserved throughout evolution in a variety of factors known to play important roles in development and cell regulation. Ldb genes encode LIM domain-binding (Ldb) factors. Here we report on the structural organization and chromosomal localization of the mouse Ldb1 gene...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1997.5163
更新日期:1998-02-15 00:00:00
abstract::The intron-containing gene for human ribosomal protein RPL23A has been cloned, sequenced, and localized. The gene is approximately 4.0 kb in length and contains five exons and four introns. All splice sites exactly match the AG/GT consensus rule. The transcript is about 0.6 kb and is detected in all tissues examined. ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1997.5038
更新日期:1997-12-01 00:00:00
abstract::Amyloid beta protein (beta A4), the major component of the core of amyloid plaques in Alzheimer disease, is derived from the transmembrane amyloid precursor proteins (APPs). Our recent studies showed that a murine member of the evolutionarily conserved APP family, amyloid precursor-like protein 1 (APLP1), is specifica...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1996.0096
更新日期:1996-02-15 00:00:00
abstract::Using cDNA probes obtained from library screening and anchored polymerase chain reaction, we have isolated and characterized three overlapping mouse genomic clones that contain the mouse lipocortin I (Lipo I) structural gene. Restriction enzyme mapping, Southern blotting, and DNA sequencing were carried out on the clo...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(91)90321-5
更新日期:1991-06-01 00:00:00
abstract::Williams syndrome (WS) is a multisystem developmental disorder caused by the deletion of contiguous genes at 7q11.23. Hemizygosity of the elastin (ELN) gene can account for the vascular and connective tissue abnormalities observed in WS patients, but the genes that contribute to features such as infantile hypercalcemi...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1996.0469
更新日期:1996-09-01 00:00:00
abstract::A human mitochondrial DNA (mtDNA) standard reference material (SRM 2392) will provide quality control when mtDNA is sequenced for forensic identifications, medical diagnosis, or mutation detection. SRM 2392 includes DNA from two lymphoblast cell cultures (CHR and 9947A) and cloned DNA from the CHR HV1 region, which co...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1998.5513
更新日期:1999-01-15 00:00:00
abstract::The cytotoxic activity of mouse natural killer cells is regulated in part through cell surface molecules belonging to the Ly49 multigene family. In mice, the genomic sequence of the Ly49 gene cluster has been examined in detail and this analysis provided a model of the expansion of this multigene family. In the presen...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2004.01.010
更新日期:2004-07-01 00:00:00
abstract::As the exponential growth of DNA sequence information in databases continues, the task of converting this deposited information into knowledge becomes more dependent on integrative sequence analysis and visualization tools. PANORAMA is an Internet-accessible software package that performs a variety of informatics anal...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.2000.6359
更新日期:2000-12-15 00:00:00
abstract::Evi9, a common site of retroviral integration in BXH2 murine myeloid leukemias, encodes a C2H2 zinc finger protein and is overexpressed in these leukemic cells. To investigate a possible role of EVI9 in the human hematopoietic system, we isolated the cDNA clone of the human homologue. Human EVI9, located on the chromo...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.2000.6385
更新日期:2000-12-15 00:00:00
abstract::The hemopoietic growth factors interleukin-3 (IL-3, multi-CSF) and granulocyte-macrophage colony-stimulating factor (GM-CSF) belong to a family of secreted glycoproteins that stimulate the proliferation and differentiation of hemopoietic progenitor cells. IL-3 and GM-CSF have overlapping biological activities and show...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(89)90070-0
更新日期:1989-08-01 00:00:00
abstract::The Long-Evans Cinnamon (LEC) rat has a spontaneous mutation, T helper immunodeficiency (thid), which causes a markedly reduced CD4(+) thymocyte population. Here we positionally clone the locus and identify a deletion in the gene encoding a receptor-like protein tyrosine phosphatase kappa (Ptprk) that led to complete ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2007.03.001
更新日期:2007-06-01 00:00:00
abstract::PKD1, the gene for the chromosome 16-linked form of autosomal dominant polycystic kidney disease, has previously been genetically mapped to an interval bounded by the polymorphic loci Fr3-42/EKMDA2 distally and O327hb/O90a proximally. More recently, 26.6PROX was identified as the closest proximal flanking locus. We se...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(92)90215-e
更新日期:1992-05-01 00:00:00
abstract::Uroporphyrinogen-III (URO) synthase is the heme biosynthetic enzyme defective in congenital erythropoietic porphyria. The approximately 34-kb human URO-synthase gene (UROS) was isolated, and its organization and tissue-specific expression were determined. The gene had two promoters that generated housekeeping and eryt...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.2000.6373
更新日期:2000-12-01 00:00:00
abstract::RFX1 is a transacting DNA-binding regulatory factor involved in the control of MHC class II gene expression. RFX2 is a structurally very similar protein with identical DNA binding features. A member of the family of RFX factors is affected in an autosomal recessive disease, MHC class II deficient combined immunodefici...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(92)90052-t
更新日期:1992-08-01 00:00:00
abstract::The human DAZ (deleted in azoospermia) gene family on the Y chromosome and an autosomal DAZ-like gene, DAZL1, encode RNA-binding proteins that are expressed exclusively in germ cells. Their role in spermatogenesis is supported by their homology with a Drosophila male infertility gene boule and sterility of Daz11 knock...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.2000.6169
更新日期:2000-05-01 00:00:00
abstract::We report the results of a chemogenomic profiling aimed to explore the mode of action of a quinolic analogue of the p300 histone acetyltransferase (HAT) inhibitor anacardic acid, named MC1626. This compound reduced histone H3 acetylation in a dose-dependent manner and the HATs Gcn5 and Rtt109, which specifically targe...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2010.08.005
更新日期:2010-11-01 00:00:00
abstract::Intercellular adhesion molecule-1 (ICAM-1, CD54) is a cell adhesion molecule that interacts with the leukocyte beta 2 integrins, LFA-1 and Mac-1. Murine inflammatory models are being utilized increasingly to define the role that ICAM-1 induction plays in the initiation of inflammation. We have isolated murine genomic ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/s0888-7543(05)80132-6
更新日期:1992-12-01 00:00:00
abstract::Vertebrate tissue inhibitors of metalloproteinases (TIMPs) regulate extracellular matrix metalloproteinases and are thus involved in a wide variety of developmental and physiological processes. By identifying cDNAs of a transcript detected within an intron of the Drosophila synapsin gene we have cloned the Drosophila ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1999.5776
更新日期:1999-04-15 00:00:00
abstract::Heterotrimeric G proteins, composed of alpha, beta, and gamma subunits, transduce signals from transmembrane receptors to a wide range of intracellular effectors. The G protein gamma subunits, which play an indispensible role in this communication, constitute a large and diverse multigene family. Using an interspecifi...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1999.5763
更新日期:1999-04-01 00:00:00
abstract::To identify novel genes regulating the biologic response to lipopolysaccharide (LPS), we used a combination of quantitative trait locus (QTL) analysis and microarray-based gene expression studies of C57BL/6J x DBA/2J(BXD) F2 and recombinant inbred (RI) mice. A QTL affecting pulmonary TNF-alpha production was identifie...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2003.12.008
更新日期:2004-06-01 00:00:00
abstract::To refine the linkage map of mouse Chromosome (Chr) 12 and to define better the homology relationships between it and human chrs 2p and 14q, nine new anonymous DNA markers of Chr 12 were identified, and mouse loci homologous to the human D14S17, CHGA, HSPA2, RRM2, TPO, and ZFP50 ("KUP") genes were defined. The inherit...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1994.1234
更新日期:1994-05-01 00:00:00
abstract::The myotonic dystrophy (DM) region has been recently shown to be bracketed by two key recombinant events. One recombinant occurs in a Dutch DM family, which maps the DM locus distal to the ERCC1 gene and D19S115 (pE0.8). The other recombinant event is in a French Canadian DM family, which maps DM proximal to D19S51 (p...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(92)90119-d
更新日期:1992-07-01 00:00:00