Abstract:
:Physical maps are important resources both in sequencing and in functional analyses of large genomes. Global contig-building approaches are regarded to be more efficient relative to the cumulative outcome of scattered and more localized physical mapping studies accompanying positional cloning. This work is part of an effort to assemble a complete physical map of mouse chromosome 11 in which selection of clones containing specific genetic markers from genomic libraries is the first step in the process. Using a previously developed strategy, we identified 361 bacterial artificial chromosomes (BACs) containing 88 gene markers. Since the linkage positions of markers chosen for these studies are known, the BAC framework obtained is anchored to the genetic map and represents about 13% of the length of the entire chromosome. Together with similar assignments of BACs generated previously using D11Mit markers (Cai et al., 1988, Genomics, 54: 387-397), 36-40% of the chromosome 11 is now assembled into contigs, and these contigs correlate through 51 clones carrying both gene and simple sequence length polymorphism markers.
journal_name
Genomicsjournal_title
Genomicsauthors
Klysik J,Cai WW,Yang C,Bradley Adoi
10.1006/geno.1999.5973subject
Has Abstractpub_date
1999-11-15 00:00:00pages
123-8issue
1eissn
0888-7543issn
1089-8646pii
S0888-7543(99)95973-6journal_volume
62pub_type
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