Detection of single DNA base mutations with mismatch repair enzymes.

Abstract:

:A novel method for identifying DNA point mutations has been developed by using mismatch repair enzymes. The high specificity of the Escherichia coli MutY protein has permitted the development of a reliable and sensitive method for the detection and characterization of point mutations in the human genome. The MutY protein is involved in a repair pathway that can convert A/G or A/C mismatches to C/G or G/C basepairs, respectively. A/G or A/C mismatches formed by hybridization between two amplified genomic DNA samples or between specific DNA probes and target DNA are nicked at the mispaired adenine strand by MutY protein. As little as 1% of the mutant sequence can be detected by the mismatch repair enzyme cleavage (MREC) method in a mixture of normal and mutated DNAs (e.g., mutant cells are only present in 1% of the normal cell background). By using different probes, the assay also can determine the nucleotide sequence of the mutation. We have applied this method to detect single-base substitutions in human oncogenes.

journal_name

Genomics

journal_title

Genomics

authors

Lu AL,Hsu IC

doi

10.1016/s0888-7543(05)80213-7

subject

Has Abstract

pub_date

1992-10-01 00:00:00

pages

249-55

issue

2

eissn

0888-7543

issn

1089-8646

pii

S0888-7543(05)80213-7

journal_volume

14

pub_type

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