Correlation of serpin-protease expression by comparative analysis of real-time PCR profiling data.


:Imbalanced protease activity has long been recognized in the progression of disease states such as cancer and inflammation. Serpins, the largest family of endogenous protease inhibitors, target a wide variety of serine and cysteine proteases and play a role in a number of physiological and pathological states. The expression profiles of 20 serpins and 105 serine and cysteine proteases were determined across a panel of normal and diseased human tissues. In general, expression of serpins was highly restricted in both normal and diseased tissues, suggesting defined physiological roles for these protease inhibitors. A high correlation in expression for a particular serpin-protease pair in healthy tissues was often predictive of a biological interaction. The most striking finding was the dramatic change observed in the regulation of expression between proteases and their cognate inhibitors in diseased tissues. The loss of regulated serpin-protease matched expression may underlie the imbalanced protease activity observed in pathological states.






Badola S,Spurling H,Robison K,Fedyk ER,Silverman GA,Strayle J,Kapeller R,Tsu CA




Has Abstract


2006-08-01 00:00:00
















  • A physical and transcript map based upon refinement of the critical interval for PPH1, a gene for familial primary pulmonary hypertension. The International PPH Consortium.

    abstract::Primary pulmonary hypertension (PPH), an often fatal disorder, is characterized by sustained elevation of pulmonary artery pressure of unknown cause. In its familial form (FPPH), the disorder segregates as an autosomal dominant and displays markedly reduced penetrance. A gene for FPPH was previously localized to a 25-...


    pub_type: 杂志文章


    authors: Machado RD,Pauciulo MW,Fretwell N,Veal C,Thomson JR,Vilariño Güell C,Aldred M,Brannon CA,Trembath RC,Nichols WC

    更新日期:2000-09-01 00:00:00

  • A YAC contig joining the desmocollin and desmoglein loci on human chromosome 18 and ordering of the desmocollin genes.

    abstract::The desmocollins and desmogleins are members of the cadherin family of adhesive proteins present in the desmosome type of cell-cell junction. All of the known desmoglein and desmocollin isoforms, which have differing tissue and developmental distributions, are coded by very closely linked genes at 18q12.1. We have pre...


    pub_type: 杂志文章


    authors: Cowley CM,Simrak D,Marsden MD,King IA,Arnemann J,Buxton RS

    更新日期:1997-06-01 00:00:00

  • Exploring the multi-drug resistance in Escherichia coli O157:H7 by gene interaction network: A systems biology approach.

    abstract::In the present study, we have constructed an interaction network of 29 antibiotic resistant genes along with 777 interactions in E. coli O157:H7. Gene ontology analysis reveals that 94, 89 and 67 genes have roles in the cellular process, biological process and molecular function respectively. Gene complexes related to...


    pub_type: 杂志文章


    authors: Miryala SK,Ramaiah S

    更新日期:2019-07-01 00:00:00

  • Mapping of equine cerebellar abiotrophy to ECA2 and identification of a potential causative mutation affecting expression of MUTYH.

    abstract::Equine Cerebellar Abiotrophy (CA) is a neurological disease found in Arabian horses. CA is characterized by post-natal degeneration of the Purkinje cells of the cerebellum. Signs of CA include ataxia, head tremors, and a lack of balance equilibrium. We have discovered a linkage of the CA phenotype to a microsatellite ...


    pub_type: 杂志文章


    authors: Brault LS,Cooper CA,Famula TR,Murray JD,Penedo MC

    更新日期:2011-02-01 00:00:00

  • Construction of a gene map of the nephronophthisis type 1 (NPHP1) region on human chromosome 2q12-q13.

    abstract::A gene for the autosomal recessive kidney disorder juvenile nephronophthisis (NPH) is located on chromosome 2q between markers D2S1893 and D2S1888. Recently, the presence of large homozygous deletions was described in the majority of NPH patients. We constructed an integrated YAC/PAC contig of 54 markers and 30 PAC cl...


    pub_type: 杂志文章


    authors: Nothwang HG,Stubanus M,Adolphs J,Hanusch H,Vossmerbäumer U,Denich D,Kübler M,Mincheva A,Lichter P,Hildebrandt F

    更新日期:1998-01-15 00:00:00

  • cDNA and genomic cloning of human palmitoyl-protein thioesterase (PPT), the enzyme defective in infantile neuronal ceroid lipofuscinosis.

    abstract::Palmitoyl-protein thioesterase (PPT) is a small glycoprotein that removes palmitate groups from cysteine residues in lipid-modified proteins. We recently reported mutations in PPT in patients with infantile neuronal ceroid lipofuscinosis (INCL), a severe neurodegenerative disorder (J. Vesa et al., 1995, Nature 376: 58...


    pub_type: 杂志文章


    authors: Schriner JE,Yi W,Hofmann SL

    更新日期:1996-06-15 00:00:00

  • A genetic map of mouse chromosome 1 near the Lsh-Ity-Bcg disease resistance locus.

    abstract::Isozyme and restriction fragment length polymorphism (RFLP) analyses of backcross progeny, recombinant inbred strains, and congenic strains of mice positioned eight genetic markers with respect to the Lsh-Ity-Bcg disease resistance locus. Allelic isoforms of Idh-1 and Pep-3 and RFLPs detected by Southern hybridization...


    pub_type: 杂志文章


    authors: Mock B,Krall M,Blackwell J,O'Brien A,Schurr E,Gros P,Skamene E,Potter M

    更新日期:1990-05-01 00:00:00

  • Molecular cloning and characterization of canine ICOS.

    abstract::Inducible costimulatory receptor (ICOS) is one recently identified member of the CD28 family of costimulatory molecules. Evidence suggests ICOS functions as a critical immune regulator and, to evaluate these effects, we employed the canine model system that has been used to develop strategies currently in clinical use...


    pub_type: 杂志文章


    authors: Lee JH,Joo YD,Yim D,Lee R,Ostrander EA,Loretz C,Little MT,Storb R,Kuhr CS

    更新日期:2004-10-01 00:00:00

  • Identification of mesoderm development (mesd) candidate genes by comparative mapping and genome sequence analysis.

    abstract::The proximal albino deletions identify several functional regions on mouse Chromosome 7 critical for differentiation of mesoderm (mesd), development of the hypothalamus neuroendocrine lineage (nelg), and function of the liver (hsdr1). Using comparative mapping and genomic sequence analysis, we have identified four nov...


    pub_type: 杂志文章


    authors: Wines ME,Lee L,Katari MS,Zhang L,DeRossi C,Shi Y,Perkins S,Feldman M,McCombie WR,Holdener BC

    更新日期:2001-02-15 00:00:00

  • Genome-scale screening and molecular characterization of membrane-bound transcription factors in Arabidopsis and rice.

    abstract::Controlled proteolytic activation of membrane-bound transcription factors (MTFs) is recently emerging as a versatile way of rapid transcriptional responses to environmental changes in plants. Here, we report genome-scale identification of putative MTFs in the Arabidopsis and rice genomes. The Arabidopsis and rice geno...


    pub_type: 杂志文章


    authors: Kim SG,Lee S,Seo PJ,Kim SK,Kim JK,Park CM

    更新日期:2010-01-01 00:00:00

  • A map of the distal region of the long arm of human chromosome 21 constructed by radiation hybrid mapping and pulsed-field gel electrophoresis.

    abstract::We have used radiation hybrid (RH) mapping and pulsed-field gel electrophoresis (PFGE) to determine the order and positions of 28 DNA markers from the distal region of the long arm of human chromosome 21. The maps generated by these two methods are in good agreement. This study, combined with that of D. R. Cox et al. ...


    pub_type: 杂志文章


    authors: Burmeister M,Kim S,Price ER,de Lange T,Tantravahi U,Myers RM,Cox DR

    更新日期:1991-01-01 00:00:00

  • Genomic cloning of mouse MIF (macrophage inhibitory factor) and genetic mapping of the human and mouse expressed gene and nine mouse pseudogenes.

    abstract::The single functional mouse gene for MIF (macrophage migration inhibitory factor) has been cloned from a P1 library, and its exon/intron structure determined and shown to resemble that of the human gene. The gene was mapped to chromosome 10 using two multilocus crosses between laboratory strains and either Mus musculu...


    pub_type: 杂志文章


    authors: Kozak CA,Adamson MC,Buckler CE,Segovia L,Paralkar V,Wistow G

    更新日期:1995-06-10 00:00:00

  • Analysis of expressed sequence tags from a fetal human heart cDNA library.

    abstract::Single-pass sequencing of randomly selected cDNA clones to generate expressed sequence tags (ESTs) has been widely used to identify novel genes and to study gene expression in a variety of tissues. We have generated 2244 ESTs from a human fetal heart library (GenBank Accession Nos. R30692-30774 and R56965-58824), whic...


    pub_type: 杂志文章


    authors: Hwang DM,Fung YW,Wang RX,Laurenssen CM,Ng SH,Lam WY,Tsui KW,Fung KP,Waye M,Lee CY

    更新日期:1995-11-20 00:00:00

  • Detection of obesity QTLs on mouse chromosomes 1 and 7 by selective DNA pooling.

    abstract::The inheritance of obesity has been analyzed in an intercross between the lean 129/Sv mouse strain and the obesity-prone EL/Suz mouse strain. The weights of three major fat pads were determined on 4-month-old mice, and the sum of these weights, divided by body weight, was used as an adiposity index. The strategy of se...


    pub_type: 杂志文章


    authors: Taylor BA,Phillips SJ

    更新日期:1996-06-15 00:00:00

  • Characterization of the adrenoleukodystrophy-related (ALDR, ABCD2) gene promoter: inductibility by retinoic acid and forskolin.

    abstract::The adrenoleukodystrophy-related gene (ALDR, ABCD2) is a candidate modifier gene and a potential therapeutic target for X-linked adrenoleukodystrophy (ALD), a severe neurodegenerative disease. The ALDR gene is the closest homologue of the ALD gene, which encodes a peroxisomal ABC transporter involved in the catabolism...


    pub_type: 杂志文章


    authors: Pujol A,Troffer-Charlier N,Metzger E,Chimini G,Mandel JL

    更新日期:2000-11-15 00:00:00

  • CancerProView: a graphical image database of cancer-related genes and proteins.

    abstract::We have developed a graphical image database CancerProView (URL: to assist the search for alterations of the motifs/domains in the cancer-related proteins that are caused by mutations in the corresponding genes. For the CancerProView, we have collected various kind...


    pub_type: 杂志文章


    authors: Mitsuyama S,Shimizu N

    更新日期:2012-08-01 00:00:00

  • Localization of the human tripeptidyl peptidase II gene (TPP2) to 13q32-q33 by nonradioactive in situ hybridization and somatic cell hybrids.

    abstract::We have assigned the human tripeptidyl peptidase II (TPP2) gene to chromosome region 13q32-q33 using two different methods. First, a full-length TPP2 cDNA was used as a probe on Southern blots of DNA from a panel of human/rodent somatic cell hybrids. The TPP2 sequences were found to segregate with the human chromosome...


    pub_type: 杂志文章


    authors: Martinsson T,Vujic M,Tomkinson B

    更新日期:1993-08-01 00:00:00

  • Expression analysis, genomic structure, and mapping to 7q31 of the human sperm adhesion molecule gene SPAM1.

    abstract::During the course of systematic sequence tag analysis of clones isolated from an adult testis cDNA library, clones 296 and 576 were found to detect 71-74% sequence identity to the guinea pig sperm surface protein PH-20. This surface protein is involved in sperm-egg adhesion in the guinea pig. Nucleotide sequence for 1...


    pub_type: 杂志文章


    authors: Jones MH,Davey PM,Aplin H,Affara NA

    更新日期:1995-10-10 00:00:00

  • A substitution mutation in the myosin binding protein C gene in ragdoll hypertrophic cardiomyopathy.

    abstract::Familial hypertrophic cardiomyopathy (HCM) is a primary myocardial disease with a prevalence of 1 in 500 in human beings. Causative mutations have been identified in several sarcomeric genes, including the cardiac myosin binding protein C (MYBPC3) gene. Heritable HCM also exists in a large-animal model, the cat, and w...


    pub_type: 杂志文章


    authors: Meurs KM,Norgard MM,Ederer MM,Hendrix KP,Kittleson MD

    更新日期:2007-08-01 00:00:00

  • The gene encoding protein 4.2 is distinct from the mouse platelet storage pool deficiency mutation pallid.

    abstract::Previous studies identified the gene encoding the erythrocyte membrane protein 4.2 (Epb4.2) as a candidate for the mouse mutation pallid (pa); Epb4.2 genetically colocalized near pa on mouse Chromosome 2, and a truncated Epb4.2 transcript was present in tissues derived from pallid mice. We report here evidence that Ep...


    pub_type: 杂志文章


    authors: Gwynn B,Korsgren C,Cohen CM,Ciciotte SL,Peters LL

    更新日期:1997-06-15 00:00:00

  • A first comparative map of copy number variations in the sheep genome.

    abstract::We carried out a cross species cattle-sheep array comparative genome hybridization experiment to identify copy number variations (CNVs) in the sheep genome analysing ewes of Italian dairy or dual-purpose breeds (Bagnolese, Comisana, Laticauda, Massese, Sarda, and Valle del Belice) using a tiling oligonucleotide array ...


    pub_type: 杂志文章


    authors: Fontanesi L,Beretti F,Martelli PL,Colombo M,Dall'olio S,Occidente M,Portolano B,Casadio R,Matassino D,Russo V

    更新日期:2011-03-01 00:00:00

  • Minimum error calibration and normalization for genomic copy number analysis.

    abstract:BACKGROUND:Copy number variations (CNV) are regional deviations from the normal autosomal bi-allelic DNA content. While germline CNVs are a major contributor to genomic syndromes and inherited diseases, the majority of cancers accumulate extensive "somatic" CNV (sCNV or CNA) during the process of oncogenetic transforma...


    pub_type: 杂志文章


    authors: Gao B,Baudis M

    更新日期:2020-09-01 00:00:00

  • The Mass1frings mutation underlies early onset hearing impairment in BUB/BnJ mice, a model for the auditory pathology of Usher syndrome IIC.

    abstract::The human ortholog of the gene responsible for audiogenic seizure susceptibility in Frings and BUB/BnJ mice (mouse gene symbol Mass1) recently was shown to underlie Usher syndrome type IIC (USH2C). Here we report that the Mass1frings mutation is responsible for the early onset hearing impairment of BUB/BnJ mice. We fo...


    pub_type: 杂志文章


    authors: Johnson KR,Zheng QY,Weston MD,Ptacek LJ,Noben-Trauth K

    更新日期:2005-05-01 00:00:00

  • A genetic linkage map of 17 markers on human chromosome 21.

    abstract::We have constructed a genetic linkage map of 17 markers on the long arm of human chromosome 21, including six genes and two anonymous loci with a variable number of tandem repeats. The estimated length of the map is 103 cM in males and 140 cM in females, assuming Kosambi interference. Recombination in females was appr...


    pub_type: 杂志文章


    authors: Warren AC,Slaugenhaupt SA,Lewis JG,Chakravarti A,Antonarakis SE

    更新日期:1989-05-01 00:00:00

  • Characterization of a novel cation transporter ATPase gene (ATP13A4) interrupted by 3q25-q29 inversion in an individual with language delay.

    abstract::Specific language impairment (SLI) is defined as failure to acquire normal language skills despite adequate intelligence and environmental stimulation. Although SLI disorders are often heritable, the genetic basis is likely to involve a number of risk factors. This study describes a 7-year-old girl carrying an inherit...


    pub_type: 杂志文章


    authors: Kwasnicka-Crawford DA,Carson AR,Roberts W,Summers AM,Rehnström K,Järvelä I,Scherer SW

    更新日期:2005-08-01 00:00:00

  • The gene encoding a cationic amino acid transporter (SLC7A4) maps to the region deleted in the velocardiofacial syndrome.

    abstract::By screening an expressed sequence tag database, we identified a novel human gene, SLC7A4, encoding a solute carrier family 7 [cationic amino acid (CAA) CAT-4 transporter, y+ system] member 4. The SLC7A4 cDNA is 2325 nt long and includes 78, 1911, and 336 nt in the 5' noncoding, coding, and 3'-noncoding regions, respe...


    pub_type: 杂志文章


    authors: Sperandeo MP,Borsani G,Incerti B,Zollo M,Rossi E,Zuffardi O,Castaldo P,Taglialatela M,Andria G,Sebastio G

    更新日期:1998-04-15 00:00:00

  • Genome-wide effects of DNA methyltransferase inhibitor on gene expression in double-stranded RNA transfected porcine PK15 cells.

    abstract::Double-stranded RNA (dsRNA) is produced in host cells during viral replication. The effects of DNA demethylation on gene expression in dsRNA transfected swine cells are unclear. The study aims to profile the transcriptome changes which are induced by DNA methyltransferase inhibitor (Aza-CdR) in porcine PK15 cells tran...


    pub_type: 杂志文章


    authors: Wang X,Ao H,Zhai L,Bai L,He W,Yu Y,Wang C

    更新日期:2014-05-01 00:00:00

  • Complete structural organization of the human alpha 1 (V) collagen gene (COL5A1): divergence from the conserved organization of other characterized fibrillar collagen genes.

    abstract::Genes that encode the vertebrate fibrillar collagen types I-III have previously been shown to share a highly conserved intron/exon organization, thought to reflect common ancestry and evolutionary pressures at the protein level. We report here the complete intron/exon organization of COL5A1, the human gene that encode...


    pub_type: 杂志文章


    authors: Takahara K,Hoffman GG,Greenspan DS

    更新日期:1995-10-10 00:00:00

  • Comparative radiation hybrid map of canine chromosome 1 incorporating SNP and indel polymorphisms.

    abstract::We report a comparative map of canine chromosome 1 (CFA1) incorporating single nucleotide polymorphisms (SNPs) and insertion/deletion (indel) polymorphisms, developed by using cross-species primers, radiation hybrid analysis, and pool-and-sequence identification of genetic variations. Fifty-five genes were chosen with...


    pub_type: 杂志文章


    authors: Housley DJ,Ritzert E,Venta PJ

    更新日期:2004-08-01 00:00:00

  • Efficiency of PacBio long read correction by 2nd generation Illumina sequencing.

    abstract::Long sequencing reads offer unprecedented opportunities in analysis and reconstruction of complex genomic regions. However, the gain in sequence length is often traded for quality. Therefore, recently several approaches have been proposed (e.g. higher sequencing coverage, hybrid assembly or sequence correction) to enh...


    pub_type: 杂志文章


    authors: Mahmoud M,Zywicki M,Twardowski T,Karlowski WM

    更新日期:2019-01-01 00:00:00