Integrated analysis of mRNA and miRNA expression in response to interleukin-6 in hepatocytes.

Abstract:

:The expression of plasma proteins changes dramatically as a result of cytokine induction, particularly interleukin-6, and their levels are used as clinical markers of inflammation. miRNAs are important regulators of gene expression and play significant roles in many inflammatory diseases and processes. The interactions between miRNAs and the genes that they regulate during the acute phase response have not been investigated. We examined the effects of IL-6 stimulation on the transcriptome and miRNome of human and mouse primary hepatocytes and the HepG2 cell line. Using an integrated analysis, we identified differentially expressed miRNAs whose seed sequences are significantly enriched in the 3' untranslated regions of differentially expressed genes, many of which are involved in inflammation-related pathways. Our finding that certain miRNAs may de-repress critical acute phase proteins within acute timeframes has important biological and clinical implications.

journal_name

Genomics

journal_title

Genomics

authors

Lukowski SW,Fish RJ,Martin-Levilain J,Gonelle-Gispert C,Bühler LH,Maechler P,Dermitzakis ET,Neerman-Arbez M

doi

10.1016/j.ygeno.2015.05.001

subject

Has Abstract

pub_date

2015-08-01 00:00:00

pages

107-15

issue

2

eissn

0888-7543

issn

1089-8646

pii

S0888-7543(15)00076-2

journal_volume

106

pub_type

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