Abstract:
:The procollagen C-proteinase enhancer (PCPE) is a glycoprotein that potentiates enzymatic cleavage of the type I procollagen C-propeptide by bone morphogenetic protein-1 (BMP-1). The human PCPE gene (PCOLCE) was previously mapped to 7q22, an area frequently disrupted in uterine leiomyomata, while disruption of the rat PCPE gene leads to anchorage-independent growth and loss of contact inhibition in rat fibroblasts. Here we describe the entire intron/exon organizations of PCOLCE and the mouse PCPE gene (Pcolce) and analyze expression of PCOLCE RNA in various human adult and fetal tissues and of Pcolce RNA at various stages of mouse development. PCOLCE and Pcolce are shown to be small genes 6.0 and 6.5 kb, respectively, with a conserved intron/exon structure comprising 9 exons. A notable difference between the two genes derives from insertion of multiple Alu sequences immediately upstream and downstream and within PCOLCE. Temporal expression of PCPE mRNA is shown to differ from that of BMP-1 and type I procollagen during mouse development, consistent with possible additional functions for PCPE beyond enhancement of C-proteinase activity. Consistent with a possible role in leiomyomata, PCOLCE is shown to be expressed at relatively high levels in uterus.
journal_name
Genomicsjournal_title
Genomicsauthors
Scott IC,Clark TG,Takahara K,Hoffman GG,Greenspan DSdoi
10.1006/geno.1998.5663subject
Has Abstractpub_date
1999-01-15 00:00:00pages
229-34issue
2eissn
0888-7543issn
1089-8646pii
S0888-7543(98)95663-4journal_volume
55pub_type
杂志文章相关文献
GENOMICS文献大全abstract::Sequence tag count-based gene expression analysis is potent for the identification of candidate genes relevant to the cancerous phenotype. With the public availability of count-based data, the computational approaches for differentially expressed genes, which are mainly based on Binomial or beta-Binomial distribution,...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2009.05.003
更新日期:2009-09-01 00:00:00
abstract::The Friedreich's ataxia locus has been previously assigned to chromosome 9q 13-21.1 by the demonstration of tight linkage to two anonymous DNA markers. MCT112 (Z greater than 80, theta = 0) and DR47 (Z greater than 50, theta = 0). The absence of recombination between these three loci has prevented the resolution of ge...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(91)90224-3
更新日期:1991-01-01 00:00:00
abstract::A MspI polymorphism was detected in the beta-glucocerebrosidase gene in 10 Swedish families affected by type III Gaucher's disease. The sizes of the polymorphic fragments were 1.70 and 1.75 kb and the disease was found to segregate with the 1.70-kb fragment in 32 meioses. Only the 1.75-kb fragment was detected in fami...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(88)90118-8
更新日期:1988-11-01 00:00:00
abstract::CircRNA is a specific type of non-coding RNA that has been shown to have an important role in mammary gland (MG) activity, but no study of MG circRNA activity in sheep so far. In this study, the expression profile of circRNAs was investigated using RNA-Seq in MG parenchyma at peak lactation from Small-Tailed Han sheep...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2019.12.014
更新日期:2020-05-01 00:00:00
abstract::A bacterial artificial chromosome (BAC) library has been constructed for use in bovine genome mapping using constructed for use in bovine genome mapping using the pBeloBAC11 vector. Currently, the library consists of 23,040 clones, which achieves a 70% probability (P=0.70) of the library containing a specific unique D...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1995.9986
更新日期:1995-09-20 00:00:00
abstract::The rat diabetes susceptibility gene, Lyp or Lymphopenia, has been localized to RNO4. Proximal to Lyp are the genes caspase-2 (Casp2) and pancreatic trypsin 1 (Prss1), while neuropeptide Y (Npy) is the closest distally positioned gene. In human, the three genes are syntenic on HSA7, but they are not on a conserved seg...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.2000.6130
更新日期:2000-04-01 00:00:00
abstract::A complete physical map of the DNA of an organism, consisting of overlapping clones spanning the genome, is an extremely useful tool for genomic analysis. Various methods for the construction of such physical maps are available. One approach is to assemble the physical map by "fingerprinting" a large number of random ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(91)90004-x
更新日期:1991-12-01 00:00:00
abstract::RNA helicase A is an enzyme that possesses both RNA and DNA helicase activities. In this report, we describe the isolation of a mouse cDNA encoding RNA helicase A. The deduced amino acid sequence derived from mouse RNA helicase A cDNA exhibits 87 and 47% identity to its human and Drosophila homologs, respectively. Usi...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1997.5139
更新日期:1998-02-01 00:00:00
abstract::The autosomal recessive mutation mnd2 results in early onset motor neuron disease with rapidly progressive paralysis, severe muscle wasting, regression of thymus and spleen, and death before 40 days of age. mnd2 has been mapped to mouse chromosome 6 with the gene order: centromere-Tcrb-Ly-2-Sftp-3-D6Mit4-mnd2-D6Mit 6,...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1993.1246
更新日期:1993-06-01 00:00:00
abstract::The vast majority of small-deletion syndromes are caused by haploinsufficiency of one or several genes and are transmitted as dominant traits. We have previously identified a homozygous deletion of 179,311 bp on chromosome 2p21 as the cause of a unique syndrome, inherited in a recessive mode, consisting of cystinuria,...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2005.04.001
更新日期:2005-08-01 00:00:00
abstract::Transcripts related to the human carcinoembryonic antigen were found in mRNA isolated from both dimethylbenzanthracene-induced and mouse mammary tumor virus-induced mammary tumors. A cDNA library was prepared from a dimethylbenzanthracene-induced tumor, and a clone was isolated by hybridization with a human carcinoemb...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(91)90439-l
更新日期:1991-07-01 00:00:00
abstract::We employed the polymerase chain reaction and genomic DNA library screening to clone novel human genes, GPR9 and GPR10, and a rat gene, GPR14. GPR9, GPR10, and GPR14 each encode G protein-coupled receptors. GPR10 and GPR14 are intronless within their coding regions, while GPR9 contains at least one intron. The recepto...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1995.9996
更新日期:1995-09-20 00:00:00
abstract::A procedure suitable for cloning labile mRNAs that contain AU motifs is presented (AU-DD). These motifs are regulatory sequences within the so-called AU-rich elements (AREs) often found in 3' untranslated regions of genes such as cytokines, proto-oncogenes, and transcription factors. AU-DD is an AU-motif-directed diff...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1998.5548
更新日期:1998-12-01 00:00:00
abstract::The lack of physical mapping data strongly restricts the analysis of the meningioma chromosomal region that was assigned to the bands 22q12.3-qter. Recently, we reported a new marker D22S16 for chromosome 22 that was assigned to the region 22q13-qter by in situ hybridization. Utilizing somatic cell hybrids we now subl...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(91)90196-l
更新日期:1991-08-01 00:00:00
abstract::Repeat element-mediated PCR can facilitate rapid cloning and mapping of human chromosomal region-specific DNA markers from somatic cell hybrid DNA. PCR primers directed to human repeat elements result in human-specific DNA synthesis; template DNA derived from a somatic cell hybrid containing the human chromosomal regi...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(92)90261-p
更新日期:1992-06-01 00:00:00
abstract::One of the major challenges in genome research is the identification of the complete set of genes in a genome. Alignments of expressed sequences (RNA and EST) with genomic sequences have been used to characterize genes. However, the number of alignments far exceeds the likely number of genes in a genome, suggesting th...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2003.07.003
更新日期:2004-04-01 00:00:00
abstract::Thirty-five new, unique, DNA probes have been isolated and each has been assigned to one of five regions on chromosome 22. The distribution of probes along the chromosome is what would be expected based on the estimated size of each region with the exception of the short arm (22p). RFLP analysis was performed using 13...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(91)90190-p
更新日期:1991-08-01 00:00:00
abstract::We have isolated and characterized genomic clones containing the mouse myelin/oligodendrocyte glycoprotein (MOG) gene. It spans a region of 12.5 kb and consists of eight exons. Its exon-intron structure differs from that of classical MHC-class I genes, with which it is linked in the mouse genome. Nucleotide sequencing...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1994.1456
更新日期:1994-09-01 00:00:00
abstract::We sequenced the mitogenomes of Astictopterus jama, Isoteinon lamprospilus and Notocrypta curvifascia to obtain further insight into the mitogenomic architecture evolution and performed phylogenetic reconstruction using 29 Hesperiidae mitogenome sequences. The complete mitogenome sequences of A. jama, I. lamprospilus ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2019.03.006
更新日期:2020-01-01 00:00:00
abstract::We report a genetic linkage map of the pericentromeric region of the human X chromosome, extending from Xp11 to Xq13. Genetic analysis with five polymorphic markers, including centromeric alpha satellite DNA, spanned a distance of approximately 38 cM. Significant lod scores were obtained with linkage analysis in 26 fa...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(88)90017-1
更新日期:1988-05-01 00:00:00
abstract::A human clone corresponding to the gene for the DNA-binding factor LFB3, a protein highly homologous to the liver-specific transcription factor LFB1, has been isolated and partially sequenced. This gene is designated TCF2. Oligonucleotide primers have been designed for LFB3 and used to amplify specifically the human g...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(90)90239-q
更新日期:1990-09-01 00:00:00
abstract::TR4 is a new member of the nuclear hormone receptor family. This receptor is highly conserved in rat and human, but an in-frame insertion of 19 amino acid residues in the amino-terminal (A/B) region was found in the human homolog, which we refer to as hTR4alpha1. By reverse transcription-PCR (RT-PCR) we have identifie...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1996.0368
更新日期:1996-07-15 00:00:00
abstract::MicroRNAs control gene expression at the posttranscriptional level by base-pairing to the 3'-UTR of their target mRNAs, thus leading to mRNA degradation of protein fabrication. We hypothesize, SNPs within miRNAs and their targets could be of significance to an individual's risk of developing cancer. We analyzed in sil...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2011.06.005
更新日期:2011-09-01 00:00:00
abstract::Deletions giving rise to Duchenne muscular dystrophy (DMD) and the less severe Becker muscular dystrophy (BMD) occur in the same large gene on the short arm of the human X chromosome. We present a molecular mechanism to explain the clinical difference in severity between DMD and BMD patients who bear partial deletions...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(88)90113-9
更新日期:1988-01-01 00:00:00
abstract::Thirty-eight kilobases of mouse genomic DNA which surround and include the coding sequences for beta-glucuronidase has been mapped. Intron-exon arrangements were determined by hybridization of genomic sequences with cDNA clones, and minimum estimates of gene length (11-17 kb) and intron number were obtained. Only a si...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(88)90105-x
更新日期:1988-01-01 00:00:00
abstract::Properdin is a serum protein belonging to the alternative pathway of complement activation whose absence is often associated with fatal bacterial infections. Properdin deficiency segregates with an X-linked recessive pattern and its position has been recently refined by genetic linkage analysis to the proximal part of...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(89)90085-2
更新日期:1989-07-01 00:00:00
abstract::Mefloquine is associated with adverse neurological effects that are mediated via unknown mechanisms. Recent in vitro studies have shown that mefloquine disrupts neuronal calcium homeostasis via liberation of the endoplasmic reticulum (ER) store and induction of calcium influx across the plasma membrane. In the present...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2005.07.004
更新日期:2005-11-01 00:00:00
abstract::The myotonic dystrophy (DM) region has been recently shown to be bracketed by two key recombinant events. One recombinant occurs in a Dutch DM family, which maps the DM locus distal to the ERCC1 gene and D19S115 (pE0.8). The other recombinant event is in a French Canadian DM family, which maps DM proximal to D19S51 (p...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(92)90119-d
更新日期:1992-07-01 00:00:00
abstract::The human HYAL2 gene encodes a lysosomal hyaluronidase that is related to the testicular PH-20 hyaluronidase. Regions conserved in these proteins have been used to design PCR primers suitable for the isolation of a fragment of the murine Hyal2 gene. This fragment was used to isolate the Hyal2 cDNA from a cDNA library....
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1998.5472
更新日期:1998-10-15 00:00:00
abstract::Differential epigenetic modification by methylation of CpG dinucleotides is a candidate mechanism that may identify the alleles of imprinted genes and result in monoallelic expression of either the maternal or the paternal allele. Determination of the allelic methylation status of imprinted genes in the gametes and du...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1998.5570
更新日期:1998-12-15 00:00:00