Abstract:
:Differential epigenetic modification by methylation of CpG dinucleotides is a candidate mechanism that may identify the alleles of imprinted genes and result in monoallelic expression of either the maternal or the paternal allele. Determination of the allelic methylation status of imprinted genes in the gametes and during early development is constrained by the limiting quantities of genomic DNA available from these early developmental stages. To circumvent this problem we have used bisulfite genomic sequencing to determine the allelic methylation status of the minimal promoter and a 1-kb region within the Xist gene during preimplantation development. We find that the parental Xist alleles are not differentially methylated in these regions. Our findings are discussed in the context of previous conflicting data obtained using methylation-sensitive restriction enzyme digestion followed by PCR amplification to assay for methylation.
journal_name
Genomicsjournal_title
Genomicsauthors
McDonald LE,Paterson CA,Kay GFdoi
10.1006/geno.1998.5570subject
Has Abstractpub_date
1998-12-15 00:00:00pages
379-86issue
3eissn
0888-7543issn
1089-8646pii
S0888-7543(98)95570-7journal_volume
54pub_type
杂志文章相关文献
GENOMICS文献大全abstract::The retina and its adjacent supporting tissues - retinal pigmented epithelium (RPE) and choroid - are critical structures in human eyes required for normal visual perception. Abnormal changes in these layers have been implicated in diseases such as age-related macular degeneration and glaucoma. With the advent of high...
journal_title:Genomics
pub_type: 杂志文章,评审
doi:10.1016/j.ygeno.2015.01.008
更新日期:2015-05-01 00:00:00
abstract::Polymorphic loci that lie at the two extremities of the Duchenne/Becker muscular dystrophy (DMD/BMD) gene have been used to estimate intragenic recombination rates. Multipoint linkage analysis of the CEPH panel of families suggests a total intragenic recombination frequency of nearly 0.12 (confidence intervals 0.041-0...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(90)90205-9
更新日期:1990-08-01 00:00:00
abstract::Traditionally, somatic mutations are detected by examining DNA sequence. The maturity of sequencing technology has allowed researchers to screen for somatic mutations in the whole genome. Increasingly, researchers have become interested in identifying somatic mutations through RNAseq data. With this motivation, we eva...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2016.03.006
更新日期:2016-05-01 00:00:00
abstract::The intron-containing gene for human ribosomal protein RPL23A has been cloned, sequenced, and localized. The gene is approximately 4.0 kb in length and contains five exons and four introns. All splice sites exactly match the AG/GT consensus rule. The transcript is about 0.6 kb and is detected in all tissues examined. ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1997.5038
更新日期:1997-12-01 00:00:00
abstract::A cosmid library has been constructed with DNA isolated from a mouse/human hybrid cell line designated A15, which was previously characterized and shown to retain chromosome 15 as the only human material. The library was generated and stored as 34 independent pools of primary colonies at 8-10,000 colonies per pool. Sc...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1996.4517
更新日期:1997-02-15 00:00:00
abstract::We have developed a high-information-content fingerprinting (HICF) system for bacterial artificial chromosome (BAC) clones using a Type IIS restriction endonuclease, HgaI, paired with a Type II restriction endonuclease, RsaI. In the method described, unknown five-base overhangs generated with HgaI are partially or ful...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.2001.6547
更新日期:2001-06-01 00:00:00
abstract::We describe the construction and characterization of methylation-resistant sequence-tagged NotI linking clones specific for the X chromosome, referred to as NotI-BsuE linking clones. The approach consists of methylating the X-chromosome-specific cloned DNA with BsuE methylase (M. BsuE), an enzyme that methylates the f...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(91)90108-q
更新日期:1991-09-01 00:00:00
abstract::Estrogen receptor-like 1a (ESRL1a; same as estrogen receptor-related orphan receptors, ERR1) belongs to a subfamily of the nuclear receptor superfamily. We have previously shown that human ESRL1a modulates estrogen responsiveness of the lactoferrin gene promoter in transiently transfected endometrial carcinoma RL95-2 ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1997.4850
更新日期:1997-08-15 00:00:00
abstract::Protein sequences were compared among Arabidopsis, Oryza and Populus to identify differential gene (DG) sets that are in one but not the other two genomes. The DG sets were screened against a plant transcript database, the NR protein database and six newly-sequenced genomes (Carica, Glycine, Medicago, Sorghum, Vitis a...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2009.01.002
更新日期:2009-05-01 00:00:00
abstract::We have previously reported that the expression of HC gp-39, a 39-kDa secretory glycoprotein and member of the chitinase protein family, is associated with late stages of monocyte to macrophage maturation. To allow further investigations of its unique expression pattern and to facilitate studies on the regulation of t...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1997.4778
更新日期:1997-07-15 00:00:00
abstract::Identification of all the transcription factors (TFs) encoded in a given genome is a prerequisite for understanding transcriptional regulatory networks. Archaea are prokaryotes that constitute one of the three main branches of organisms with an astounding diversity of habitats. In this report, we establish the Archaea...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2007.09.007
更新日期:2008-01-01 00:00:00
abstract::Multiple infections by HPV genotypes are frequently detected in HPV+ cervical lesions but the interaction between each viral genotype during carcinogenesis is poorly understood. Here we carried out a comprehensive study to characterize the multiple HPV expression and integration by RNA-seq analyses of 19 invasive cerv...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2020.05.009
更新日期:2020-09-01 00:00:00
abstract::Chromosomal locations have been assigned to seven members of the TGF-beta superfamily using an interspecific mouse backcross. Probes for the Tgfb-1, -2, and -3, Bmp-2a and -3, and Vgr-1 genes recognized only single loci, whereas the Bmp-2b probe recognized two independently segregating loci (designated Bmp-2b1 and Bmp...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(90)90480-i
更新日期:1990-03-01 00:00:00
abstract::Hemizygous deletions on chromosome 22q11.2 result in developmental disorders referred to as DiGeorge syndrome (DGS)/velocardiofacial syndrome (VCFS). We report the isolation of a novel gene, PCQAP (PC2 glutamine/Q-rich-associated protein), that maps to the DiGeorge typically deleted region and encodes a protein identi...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.2001.6566
更新日期:2001-06-15 00:00:00
abstract::IB1/JIP-1 is a scaffold protein that regulates the c-Jun NH(2)-terminal kinase (JNK) signaling pathway, which is activated by environmental stresses and/or by treatment with proinflammatory cytokines including IL-1beta and TNF-alpha. The JNKs play an essential role in many biological processes, including the maturatio...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.2000.6129
更新日期:2000-03-15 00:00:00
abstract::In the process of cloning the gene (Scyd1) encoding the mouse CX3C chemokine fractalkine, we identified a novel cDNA that encodes a chimeric molecule termed fracTARC. This molecule is a variant form of the mouse CC chemokine, TARC (for thymus- and activation-regulated chemokine), bearing the fractalkine signal sequenc...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.2001.6585
更新日期:2001-07-01 00:00:00
abstract::Type XI collagen, a minor structural component of cartilage fibrils, is composed of three chains, alpha 1(XI), alpha 2(XI), and alpha 3(XI). Using a cloned fragment of the human alpha 2(XI) collagen gene (COL11A2) as a molecular probe for in situ hybridization and somatic cell hybrid mapping, we have localized the gen...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(89)90135-3
更新日期:1989-11-01 00:00:00
abstract::Long sequencing reads offer unprecedented opportunities in analysis and reconstruction of complex genomic regions. However, the gain in sequence length is often traded for quality. Therefore, recently several approaches have been proposed (e.g. higher sequencing coverage, hybrid assembly or sequence correction) to enh...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2017.12.011
更新日期:2019-01-01 00:00:00
abstract::We describe here a 1.2-Mb yeast artificial chromosome (YAC) contig within the region of mouse chromosome 17 between Brachyury (T) and D17Rp17e, and spanning the quaking (qk) region. We describe six new probes distributed across 1.2 Mb: D17Leh502, D17Leh503, D17Leh504, D17Leh505, D17Leh506, and D17Leh507. Probes D17Leh...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1994.1227
更新日期:1994-05-01 00:00:00
abstract::Human/rodent somatic cell hybrids have been exceedingly useful in assigning human genes and DNA sequences to specific human chromosomes. As new technologies for analyzing the human chromosome complement of such human/rodent hybrid cells become available, it is of critical importance that these be applied to enhance ch...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1993.1026
更新日期:1993-01-01 00:00:00
abstract:AIM:The objective of this study is to examine the alterations in the levels of expression of serum lncRNA-TSIX, TP53INP2 mRNA, miRNA-1283 in spinal cord injured (SCI) patients versus healthy control. METHOD:The expression of the selected RNAs in the sera was determined in 23 patients suffering from acute spinal cord i...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2020.06.018
更新日期:2020-09-01 00:00:00
abstract::A human mitochondrial DNA (mtDNA) standard reference material (SRM 2392) will provide quality control when mtDNA is sequenced for forensic identifications, medical diagnosis, or mutation detection. SRM 2392 includes DNA from two lymphoblast cell cultures (CHR and 9947A) and cloned DNA from the CHR HV1 region, which co...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1998.5513
更新日期:1999-01-15 00:00:00
abstract::To facilitate the practical application of highly efficient semiautomated methods for general application in genomic analyses, we have developed a fluorescence-based microsatellite marker resource. Ninety highly polymorphic microsatellite markers were combined to provide a rapid, accurate, and highly efficient initial...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1994.1628
更新日期:1994-11-15 00:00:00
abstract::Type V collagen is a fibrillar collagen that is widely distributed in tissues as a minor component of extracellular matrix and is usually composed of one pro alpha 2 (V) and two pro alpha 1 (V) chains. In this report, recently isolated cDNA and genomic clones, which encode the pro alpha 1 (V) chain, are used as probes...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(92)90320-r
更新日期:1992-04-01 00:00:00
abstract::A NotI end clone library has been constructed from a human-hamster hybrid cell line containing only human chromosome 11. Fifty-one NotI clones were chosen to characterize the library. The majority of NotI clones hybridize to small 15- to 200-kb fragments and have proven to be valuable for chromosome 11 physical mappin...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1993.1120
更新日期:1993-03-01 00:00:00
abstract::Most parasitic flatworms go through different life stages with important physiological and morphological changes. In this work, we used a transcriptomic approach to analyze the main life-stages of the model tapeworm Hymenolepis microstoma (eggs, cysticercoids, and adults). Our results showed massive transcriptomic cha...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2021.01.005
更新日期:2021-01-21 00:00:00
abstract::A cluster of genes coding for proteins of the extracellular matrix (ECM) containing sequence motifs essential for integrin-receptor interactions is located on HSA4q21 and on BTA6, within the critical region of a quantitative trait locus (QTL) affecting milk protein production. Genes within this cluster are involved in...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2004.03.005
更新日期:2004-08-01 00:00:00
abstract::Neuroendocrine-Dlg (NE-Dlg) is a member of the discs-large-related (DLG) subfamily of the membrane-associated guanylate kinase-related protein family. Based on evidence from model systems, this protein appears to be critical for synaptogenesis, acting as a site-specific organizational center for integral membrane prot...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1998.5243
更新日期:1998-04-15 00:00:00
abstract::Mammalian genomes include DNA segments that are imprinted (CpG-methylated) only on one of the two parental chromosomes, leading to parent-of-origin-specific gene expression. The process is regulated by Imprinting Control Regions (ICRs) and germline Differentially Methylated Regions (gDMRs). Previously, ZFP57 was shown...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2017.04.008
更新日期:2017-07-01 00:00:00
abstract::The goat beta-globin cluster is composed of a triplicated four-gene set. A locus control region (LCR) containing elements homologous to 5'DNase I hypersensitive sites (HS) 1, 2, and 3 of the human beta-globin LCR has been identified at the 5' end of this locus. We determined 10.2 kb of nucleotide sequence from the goa...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(91)90415-b
更新日期:1991-03-01 00:00:00