Abstract:
:To facilitate the practical application of highly efficient semiautomated methods for general application in genomic analyses, we have developed a fluorescence-based microsatellite marker resource. Ninety highly polymorphic microsatellite markers were combined to provide a rapid, accurate, and highly efficient initial genome-wide screening system. These markers are spaced on average every 33 cM, with a mean heterozygosity of 81% (range 65-94%), covering 22 autosomes and the X and Y chromosomes. Less than 10% of the genome lies beyond 20 cM of the nearest marker. Since this genomic analysis system is fully compatible with automated fragment analyzers using simultaneous four-color fluorescence-based detection systems, the 5 groups of 18 markers can be detected concurrently. This multiplex detection provides a through-put of 1944 genotypes daily per instrument. This system will be highly beneficial in a number of clinical and research applications including linkage, cancer genetics, forensics, and cytogenetics.
journal_name
Genomicsjournal_title
Genomicsauthors
Levitt RC,Kiser MB,Dragwa C,Jedlicka AE,Xu J,Meyers DA,Hudson JRdoi
10.1006/geno.1994.1628subject
Has Abstractpub_date
1994-11-15 00:00:00pages
361-5issue
2eissn
0888-7543issn
1089-8646pii
S0888-7543(84)71628-4journal_volume
24pub_type
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