Abstract:
:A NotI end clone library has been constructed from a human-hamster hybrid cell line containing only human chromosome 11. Fifty-one NotI clones were chosen to characterize the library. The majority of NotI clones hybridize to small 15- to 200-kb fragments and have proven to be valuable for chromosome 11 physical mapping by detecting fragments not previously recognized by random probes. These NotI end clones have been used to isolate corresponding NotI linking cosmids which were then used to identify adjacent NotI fragments on pulsed-field gels. The clones were mapped using fluorescence in situ hybridization and a somatic cell hybrid panel. Although these clones were localized over the entirety of chromosome 11, a nonrandom distribution was observed. Northern blot analysis indicated that 57% (17/30) of the NotI clones examined detected poly(A)+ transcripts in HeLa cell RNA.
journal_name
Genomicsjournal_title
Genomicsauthors
Sanford J,Kim BW,Deaven LL,Jones C,Higgins MJ,Nowak NJ,Shows TBdoi
10.1006/geno.1993.1120subject
Has Abstractpub_date
1993-03-01 00:00:00pages
653-8issue
3eissn
0888-7543issn
1089-8646pii
S0888-7543(83)71120-1journal_volume
15pub_type
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