Abstract:
:Mefloquine is associated with adverse neurological effects that are mediated via unknown mechanisms. Recent in vitro studies have shown that mefloquine disrupts neuronal calcium homeostasis via liberation of the endoplasmic reticulum (ER) store and induction of calcium influx across the plasma membrane. In the present study, global changes in gene expression induced in neurons in response to mefloquine-induced disruption of calcium homeostasis and appropriate control agents were investigated in vitro using Affymetrix arrays. The mefloquine transcriptome was found to be enriched for important regulatory sequences of the unfolded protein response and the drug was also found to induce key ER stress proteins, albeit in a manner dissimilar to, and at higher equivalent concentrations than, known ER-tropic agents like thapsigargin. Mefloquine also down-regulated several important functional categories of genes, including transcripts encoding G proteins and ion channels. These effects may be related to intrusion of extracellular calcium since they were also observed after glutamate, but not thapsigargin, hydrogen peroxide, or low-dose mefloquine treatment. Mefloquine could be successfully differentiated from other treatments on the basis of principle component analysis of its "calcium-relevant" transcriptome. These data may aid interpretation of expression of results from future in vivo studies.
journal_name
Genomicsjournal_title
Genomicsauthors
Dow GS,Caridha D,Goldberg M,Wolf L,Koenig ML,Yourick DL,Wang Zdoi
10.1016/j.ygeno.2005.07.004subject
Has Abstractpub_date
2005-11-01 00:00:00pages
539-50issue
5eissn
0888-7543issn
1089-8646pii
S0888-7543(05)00190-4journal_volume
86pub_type
杂志文章相关文献
GENOMICS文献大全abstract::Protease nexin I (PNI) is the most important physiologic regulator of alpha-thrombin in tissues. PNI is highly expressed and developmentally regulated in the nervous system where it is concentrated at neuromuscular junctions and also central synapses in the hippocampus and striatum. Approximately 10% of identified pro...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1995.1025
更新日期:1995-05-01 00:00:00
abstract::Central to reconstruction of cis-regulatory networks is identification and classification of naturally occurring transcription factor-binding sites according to the genes that they control. We have examined salient characteristics of 9-mers that occur in various orders and combinations in the proximal promoters of hum...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2004.08.013
更新日期:2004-12-01 00:00:00
abstract::The two contiguous IGF2 (human insulin-like growth factor II) and H19 genes are reciprocally imprinted in both human and mouse. In most tissues, IGF2 is transcribed only from the paternal chromosome while H19 is transcribed only from the maternal allele. The presence of a differential methylation region (DMR) on the t...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1999.6094
更新日期:2000-03-01 00:00:00
abstract::Tandem repetitive sequences are DNA motifs common in the genomes of eukaryotic species and are often embedded in heterochromatic regions. In most eukaryotes, ribosomal genes, as well as centromeres and telomeres or subtelomeres, are associated with abundant tandem arrays of repetitive sequences and typically represent...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2008.04.008
更新日期:2008-08-01 00:00:00
abstract::The cDNA for the PSST subunit of human mitochondrial nicotinamide adenine dinucleotide (NADH): ubiquinone oxidoreductase [complex I; NADH dehydrogenase (ubiquinone), Fe-S (20 kDa); EC 1.6.5.3] was generated by polymerase chain reaction (PCR) amplification of human cDNA. The sequence of the mature protein deduced from ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1996.0572
更新日期:1996-11-01 00:00:00
abstract::In extracellular fluids the insulin-like growth factors (IGFs) are bound to specific binding proteins (IGBPs). The genes for two members of this protein family have been mapped, the IGBP1 gene to human chromosomal region 7p14-p12 and the IGBP2 gene to region 2q33-q34. In this study, somatic cell hybrid analysis indica...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(92)90440-4
更新日期:1992-03-01 00:00:00
abstract::We report monozygotic twin girls with syndromic intellectual disability who underwent exome sequencing but with negative pathogenic variants. To search for variants that are unrecognized by exome sequencing, high-fidelity long-read genome sequencing (HiFi LR-GS) was applied. A 12-kb copy-neutral inversion was precisel...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2020.10.038
更新日期:2020-11-04 00:00:00
abstract::This communication describes a software tool that enables one to simulate large-scale regional mapping using an ordered shotgun sequencing approach. The analysis routines that are provided yield an estimate of the depth of coverage of the physical map, the largest contig formed, and the number of gaps remaining at any...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(95)80057-s
更新日期:1995-01-20 00:00:00
abstract::Perilipin 1 (PLIN1) protein, also known as lipid droplet-associated protein, is encoded by the PLIN1 gene and is able to anchor itself to the membranes of lipid droplets. The phosphorylation of PLIN1 is critical for the mobilization of fat in adipose tissue and plays an important role in regulating lipolysis and lipid...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2020.01.012
更新日期:2020-05-01 00:00:00
abstract::The primary structure of the human microsomal glutathione S-transferase gene (GST12) was determined by genomic cloning. The gene structure of GST12 spans 12.8 kb and consists of four exons and three introns. The coding sequence resides on exons 2, 3, and 4. Sequencing of the exons revealed two nucleotide differences c...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1996.0429
更新日期:1996-08-15 00:00:00
abstract::The human ortholog of the gene responsible for audiogenic seizure susceptibility in Frings and BUB/BnJ mice (mouse gene symbol Mass1) recently was shown to underlie Usher syndrome type IIC (USH2C). Here we report that the Mass1frings mutation is responsible for the early onset hearing impairment of BUB/BnJ mice. We fo...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2005.02.006
更新日期:2005-05-01 00:00:00
abstract::We have constructed two YAC contigs in the Xp11.23-p11.22 interval of the human X chromosome, a region that was previously poorly characterized. One contig, of at least 1.4 Mb, links the pseudogene OATL1 to the genes GATA1, TFE3, and SYP and also contains loci implicated in Wiskott-Aldrich syndrome and synovial sarcom...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1995.9976
更新日期:1995-09-20 00:00:00
abstract::Transcobalamin I (TCI) is a vitamin B12 binding protein that is found in the secondary granules of mature neutrophils. The expression of the gene for TCI (TCN1) within neutrophils has been shown to be restricted to the later stages of myeloid development and can therefore be used as a marker for granulocyte differenti...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(92)90435-u
更新日期:1992-03-01 00:00:00
abstract::The gene responsible for the second form of autosomal dominant polycystic kidney disease, PKD2, has recently been identified. We now describe the cloning, genomic localization, cDNA sequence, and expression analysis of its murine homologue, Pkd2. The cloned cDNA sequence is 5134 bp long and is predicted to encode a 96...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1997.4920
更新日期:1997-10-01 00:00:00
abstract::The sequence of the gorilla alpha-fetoprotein gene, including 869 base pairs of the 5' flanking region and 4892 base pairs of the 3' flanking region (24,607 in total), was determined from two overlapping lambda phage clones. The sequence extends 18,846 base pairs from the Cap site to the polyadenylation site, and it r...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(91)90221-y
更新日期:1991-01-01 00:00:00
abstract::The human gene encoding the muscle-specific beta-enolase has been isolated. The beta-enolase gene was mapped to chromosome 17 by analysis of a panel of rodent-human somatic cell hybrids. The gene was further localized to the short arm and tentatively to the region 17pter-p11 by analysis of cell hybrids and transfectan...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(90)90467-9
更新日期:1990-01-01 00:00:00
abstract::A locus for Usher syndrome type III (USH3; MIM No. 276902) was recently assigned to a 5-cM region on chromosome 3q. We constructed a yeast artificial chromosome contig that allowed us to position novel polymorphisms in the region. These were typed in a total of 32 pedigrees from a geographically isolated Finnish found...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1996.0626
更新日期:1996-12-15 00:00:00
abstract::Paulownia tomentosa is an important foundation forest tree species in semiarid areas. The lack of genetic information hinders research into the mechanisms involved in its response to abiotic stresses. Here, short-read sequencing technology (Illumina) was used to de novo assemble the transcriptome on P. tomentosa. A to...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2014.08.008
更新日期:2014-10-01 00:00:00
abstract::Inducible costimulatory receptor (ICOS) is one recently identified member of the CD28 family of costimulatory molecules. Evidence suggests ICOS functions as a critical immune regulator and, to evaluate these effects, we employed the canine model system that has been used to develop strategies currently in clinical use...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2004.06.009
更新日期:2004-10-01 00:00:00
abstract::Friedreich ataxia results from frataxin insufficiency caused by repeat expansion in intron 1 of the frataxin gene. Since the coding sequence is unchanged, the potential exists to ameliorate symptoms by increasing frataxin promoter activity. We therefore defined the minimal frataxin promoter in humans. Despite the fact...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2004.10.013
更新日期:2005-02-01 00:00:00
abstract::Evi9, a common site of retroviral integration in BXH2 murine myeloid leukemias, encodes a C2H2 zinc finger protein and is overexpressed in these leukemic cells. To investigate a possible role of EVI9 in the human hematopoietic system, we isolated the cDNA clone of the human homologue. Human EVI9, located on the chromo...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.2000.6385
更新日期:2000-12-15 00:00:00
abstract::Here, we report the complete genomic sequence and the characterization of the 311-kb region of 18q21, a candidate tumor suppressor locus containing a region of homozygous deletion in a lung cancer cell line, Ma29. This region contained two known genes, SMAD4 and ME2 (mitochondrial malate oxydoreductase), and two novel...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.2000.6454
更新日期:2001-03-01 00:00:00
abstract::Whole-cell fusion between zebrafish fibroblast-like ZF4 cells and mouse B78 melanoma cells resulted in hybrids containing one or a few zebrafish chromosome segments in a murine chromosomal background. Fluorescence in situ hybridization to hybrid cell metaphases with a zebrafish genomic DNA probe revealed that many hyb...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1996.0159
更新日期:1996-04-01 00:00:00
abstract::We sequenced the mitogenomes of Astictopterus jama, Isoteinon lamprospilus and Notocrypta curvifascia to obtain further insight into the mitogenomic architecture evolution and performed phylogenetic reconstruction using 29 Hesperiidae mitogenome sequences. The complete mitogenome sequences of A. jama, I. lamprospilus ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2019.03.006
更新日期:2020-01-01 00:00:00
abstract::DNA methylation is a potential epigenetic mechanism that regulates genome stability, development, and stress mitigation in plants. It is mediated by cytosine-5 DNA methyltransferases (C5-MTases). We identified 52 wheat C5-MTases; and based on domain structure and phylogenetics, these 52 C5-MTases were classified into ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2020.08.031
更新日期:2020-11-01 00:00:00
abstract::We have established a method for amplifying and obtaining large quantities of chromosome-specific DNA by linker/adaptor ligation and polymerase chain reaction (PCR). Small quantities of DNA isolated from flow cytometry-sorted chromosomes 17 and 21 were digested with MboI, ligated to a linker/adaptor, and then subjecte...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(92)90378-6
更新日期:1992-02-01 00:00:00
abstract::We examined the allele and haplotype frequencies of five polymorphic DNA markers in 355 European cystic fibrosis (CF) patients (from Belgium, the German Democratic Republic, Greece, and Italy) who were divided into two groups according to whether they were or not taking supplementary pancreatic enzymes. The level of l...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(89)90131-6
更新日期:1989-11-01 00:00:00
abstract::We describe here a 1.2-Mb yeast artificial chromosome (YAC) contig within the region of mouse chromosome 17 between Brachyury (T) and D17Rp17e, and spanning the quaking (qk) region. We describe six new probes distributed across 1.2 Mb: D17Leh502, D17Leh503, D17Leh504, D17Leh505, D17Leh506, and D17Leh507. Probes D17Leh...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1994.1227
更新日期:1994-05-01 00:00:00
abstract::Palmitoyl-protein thioesterase (PPT) is a small glycoprotein that removes palmitate groups from cysteine residues in lipid-modified proteins. We recently reported mutations in PPT in patients with infantile neuronal ceroid lipofuscinosis (INCL), a severe neurodegenerative disorder (J. Vesa et al., 1995, Nature 376: 58...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1996.0292
更新日期:1996-06-15 00:00:00
abstract::For diseases in which thrombosis plays a pivotal role, such as virus-induced fulminant hepatitis, fetal loss syndrome, and xenograft rejection, the major procoagulant has remained elusive. Here we describe the isolation and functional expression of a distinct human prothrombinase, termed FGL2. The murine fgl2 gene pro...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.2000.6444
更新日期:2001-02-01 00:00:00