Abstract:
:The complete nucleotide sequence of murine beta-glucuronidase (GUS) mRNA has been compiled from three overlapping cloned cDNAs and a single GUS-specific genomic clone. The sequence is composed of 2455 nucleotides, exclusive of the poly(A) tail. The 5' and 3' untranslated regions contain 12 and 499 bases, respectively, with the open reading frame encoding a polypeptide of 648 amino acids (74.2 kDa), including a 22 amino acid signal sequence. The nucleotide and deduced amino acid sequences of murine GUS are compared to those published for rat and human GUS and the results are presented. Murine GUS also shares amino acid sequence identity with Escherichia coli GUS and beta-galactosidase. The complete sequences of murine GUS mRNA and its deduced polypeptide provide a basis from which to study the mechanisms responsible for the well-characterized variation in GUS expression among inbred mouse strains.
journal_name
Genomicsjournal_title
Genomicsauthors
Gallagher PM,D'Amore MA,Lund SD,Ganschow REdoi
10.1016/0888-7543(88)90005-5subject
Has Abstractpub_date
1988-04-01 00:00:00pages
215-9issue
3eissn
0888-7543issn
1089-8646pii
0888-7543(88)90005-5journal_volume
2pub_type
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