Abstract:
:Fibrillin-1 is a large cysteine-rich glycoprotein of the 10-nm microfibrils in the extracellular matrix. A spectrum of mutations in the fibrillin-1 gene (FBN1) have been identified in patients with Marfan syndrome (MFS), and the majority of mutations resulting in the neonatal and often lethal form of MFS have been identified in the restricted region of exons 24-32 of the FBN1 gene. Here we report a novel point mutation in exon 25 of the FBN1 gene in a patient with lethal MFS. The mutation resulted in a molecular defect rarely encountered in human diseases, the creation of an extra consensus sequence for N-glycosylation. Metabolic labeling of the patient fibroblast culture and in vitro expression of the mutagenized cDNA construct suggest that this novel N-glycosylation site is actually utilized. Immunohistochemical and ultrastructural analyses of the fibroblast cultures of the patient show that this excessive N-glycosylation severely affects microfibril formation in vitro; this finding emphasizes the importance of correct posttranslational modifications of fibrillin molecules for correct aggregation into microfibrillar structures.
journal_name
Genomicsjournal_title
Genomicsauthors
Lönnqvist L,Karttunen L,Rantamäki T,Kielty C,Raghunath M,Peltonen Ldoi
10.1006/geno.1996.0492subject
Has Abstractpub_date
1996-09-15 00:00:00pages
468-75issue
3eissn
0888-7543issn
1089-8646pii
S0888-7543(96)90492-9journal_volume
36pub_type
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