Abstract:
:We describe the construction of a high-resolution radiation hybrid (RH) map of the domestic cat genome, which includes 2662 markers, translating to an estimated average intermarker distance of 939 kilobases (kb). Targeted marker selection utilized the recent feline 1.9x genome assembly, concentrating on regions of low marker density on feline autosomes and the X chromosome, in addition to regions flanking interspecies chromosomal breakpoints. Average gap (breakpoint) size between cat-human ordered conserved segments is less than 900 kb. The map was used for a fine-scale comparison of conserved syntenic blocks with the human and canine genomes. Corroborative fluorescence in situ hybridization (FISH) data were generated using 129 domestic cat BAC clones as probes, providing independent confirmation of the long-range correctness of the map. Cross-species hybridization of BAC probes on divergent felids from the genera Profelis (serval) and Panthera (snow leopard) provides further evidence for karyotypic conservation within felids, and demonstrates the utility of such probes for future studies of chromosome evolution within the cat family and in related carnivores. The integrated map constitutes a comprehensive framework for identifying genes controlling feline phenotypes of interest, and to aid in assembly of a higher coverage feline genome sequence.
journal_name
Genomicsjournal_title
Genomicsauthors
Davis BW,Raudsepp T,Pearks Wilkerson AJ,Agarwala R,Schäffer AA,Houck M,Chowdhary BP,Murphy WJdoi
10.1016/j.ygeno.2008.09.010subject
Has Abstractpub_date
2009-04-01 00:00:00pages
299-304issue
4eissn
0888-7543issn
1089-8646pii
S0888-7543(08)00230-9journal_volume
93pub_type
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