A high-resolution cat radiation hybrid and integrated FISH mapping resource for phylogenomic studies across Felidae.


:We describe the construction of a high-resolution radiation hybrid (RH) map of the domestic cat genome, which includes 2662 markers, translating to an estimated average intermarker distance of 939 kilobases (kb). Targeted marker selection utilized the recent feline 1.9x genome assembly, concentrating on regions of low marker density on feline autosomes and the X chromosome, in addition to regions flanking interspecies chromosomal breakpoints. Average gap (breakpoint) size between cat-human ordered conserved segments is less than 900 kb. The map was used for a fine-scale comparison of conserved syntenic blocks with the human and canine genomes. Corroborative fluorescence in situ hybridization (FISH) data were generated using 129 domestic cat BAC clones as probes, providing independent confirmation of the long-range correctness of the map. Cross-species hybridization of BAC probes on divergent felids from the genera Profelis (serval) and Panthera (snow leopard) provides further evidence for karyotypic conservation within felids, and demonstrates the utility of such probes for future studies of chromosome evolution within the cat family and in related carnivores. The integrated map constitutes a comprehensive framework for identifying genes controlling feline phenotypes of interest, and to aid in assembly of a higher coverage feline genome sequence.






Davis BW,Raudsepp T,Pearks Wilkerson AJ,Agarwala R,Schäffer AA,Houck M,Chowdhary BP,Murphy WJ




Has Abstract


2009-04-01 00:00:00
















  • A mutation in apolipoprotein A-I in the Iowa type of familial amyloidotic polyneuropathy.

    abstract::Immunoblotting of isoelectric focusing gels of plasma and direct genomic DNA sequencing have been used to characterize a mutation in apolipoprotein A-I associated with the familial amyloidotic polyneuropathy originally described by Van Allen in an Iowa kindred. An arginine for glycine substitution in apolipoprotein A-...


    pub_type: 杂志文章


    authors: Nichols WC,Gregg RE,Brewer HB Jr,Benson MD

    更新日期:1990-10-01 00:00:00

  • A 9.1-kb gap in the genome reference map is shown to be a stable deletion/insertion polymorphism of ancestral origin.

    abstract::We show a mute 9.1-kb gap in the human genome reference map, unraveled by RDA studies, to be a worldwide deletion/insertion polymorphism of stable type. The molecular and population data presented suggest its origin from a unique ancestral transposition event in chromosomal region 22q11.2, overlapping the IglambdaV ge...


    pub_type: 杂志文章


    authors: Robledo R,Orru S,Sidoti A,Muresu R,Esposito D,Grimaldi MC,Carcassi C,Rinaldi A,Bernini L,Contu L,Romani M,Roe B,Siniscalco M

    更新日期:2002-12-01 00:00:00

  • Non-coding RNAs: The key detectors and regulators in cardiovascular disease.

    abstract::Cardiovascular disease (CVD) is an important cause of disease-related death worldwide. One of its main pathological bases is imbalances in gene expression. Non-coding RNAs are a class of transcripts that do not encode proteins. They include microRNA (miRNA), long noncoding RNA (lncRNA) and circular RNA (circRNA). They...


    pub_type: 杂志文章,评审


    authors: Zhu L,Li N,Sun L,Zheng D,Shao G

    更新日期:2020-10-22 00:00:00

  • iDNA6mA-PseKNC: Identifying DNA N6-methyladenosine sites by incorporating nucleotide physicochemical properties into PseKNC.

    abstract::N6-methyladenine (6mA) is one kind of post-replication modification (PTM or PTRM) occurring in a wide range of DNA sequences. Accurate identification of its sites will be very helpful for revealing the biological functions of 6mA, but it is time-consuming and expensive to determine them by experiments alone. Unfortuna...


    pub_type: 杂志文章


    authors: Feng P,Yang H,Ding H,Lin H,Chen W,Chou KC

    更新日期:2019-01-01 00:00:00

  • Transcriptional analysis of a novel cluster of LY-6 family members in the human and mouse major histocompatibility complex: five genes with many splice forms.

    abstract::Lymphocyte antigen-6 (LY-6) superfamily members are cysteine-rich, generally GPI-anchored cell surface proteins, which have definite or putative immune related roles. A cluster of five potential LY-6 superfamily members is located in the human and mouse major histocompatibility complex class III region. Comparative an...


    pub_type: 杂志文章


    authors: Mallya M,Campbell RD,Aguado B

    更新日期:2002-07-01 00:00:00

  • Single-nucleotide polymorphism alleles in the insulin receptor gene are associated with typical migraine.

    abstract::We have identified a migraine locus on chromosome 19p13.3/2 using linkage and association analysis. We isolated 48 single-nucleotide polymorphisms within the locus, of which we genotyped 24 in a Caucasian population comprising 827 unrelated cases and 765 controls. Five single-nucleotide polymorphisms within the insuli...


    pub_type: 杂志文章


    authors: McCarthy LC,Hosford DA,Riley JH,Bird MI,White NJ,Hewett DR,Peroutka SJ,Griffiths LR,Boyd PR,Lea RA,Bhatti SM,Hosking LK,Hood CM,Jones KW,Handley AR,Rallan R,Lewis KF,Yeo AJ,Williams PM,Priest RC,Khan P,Donnelly

    更新日期:2001-12-01 00:00:00

  • The gene for the human putative apoE receptor is on chromosome 12 in the segment q13-14.

    abstract::We have previously described the cDNA coding for a new lipoprotein receptor that contains domains closely related to the ligand-binding domain of the LDL receptor. We have now investigated the localization of the gene for this new receptor by hybridization of the cDNA to panels of rodent cells containing subsets of hu...


    pub_type: 杂志文章


    authors: Myklebost O,Arheden K,Rogne S,Geurts van Kessel A,Mandahl N,Herz J,Stanley K,Heim S,Mitelman F

    更新日期:1989-07-01 00:00:00

  • Linkage analysis and physical mapping near the gene for X-linked agammaglobulinemia at Xq22.

    abstract::The gene for X-linked agammaglobulinemia (XLA) has been mapped to Xq22. No recombinations have been reported between the gene and the probe p212 at DXS178; however, this probe is informative in only 30-40% of women and the reported flanking markers, DXS3 and DXS94, are 10-15 cM apart. To identify additional probes tha...


    pub_type: 杂志文章


    authors: Parolini O,Hejtmancik JF,Allen RC,Belmont JW,Lassiter GL,Henry MJ,Barker DF,Conley ME

    更新日期:1993-02-01 00:00:00

  • The ubiquitin-homology gene PIC1: characterization of mouse (Pic1) and human (UBL1) genes and pseudogenes.

    abstract::The human ubiquitin-homology domain protein PIC1 interacts with the acute promyelocytic leukemia protein PML, and both proteins form part of the large, nuclear, multiprotein complexes known as PML nuclear bodies. The normal punctate immunohistochemical staining pattern of these complexes is disrupted by viral infectio...


    pub_type: 杂志文章


    authors: Howe K,Williamson J,Boddy N,Sheer D,Freemont P,Solomon E

    更新日期:1998-01-01 00:00:00

  • Dysregulation in the expression of (lncRNA-TSIX, TP53INP2 mRNA, miRNA-1283) in spinal cord injury.

    abstract:AIM:The objective of this study is to examine the alterations in the levels of expression of serum lncRNA-TSIX, TP53INP2 mRNA, miRNA-1283 in spinal cord injured (SCI) patients versus healthy control. METHOD:The expression of the selected RNAs in the sera was determined in 23 patients suffering from acute spinal cord i...


    pub_type: 杂志文章


    authors: Salah SMM,Matboli M,Nasser HE,Abdelnaiem IA,Shafei AE,El-Asmer MF

    更新日期:2020-09-01 00:00:00

  • Cloning and gene mapping of the mouse homologue of the CBFA2T1 gene associated with human acute myeloid leukemia.

    abstract::The human CBFA2T1 (also known as MTG8) gene, on chromosome 8, has been identified through its involvement in the t(8;21) chromosomal translocation, frequently found in acute myeloid leukemia. We report here the isolation and characterization of the mouse homologue of the CBFA2T1 gene, Cbfa2t1h. Nucleotide sequence ana...


    pub_type: 杂志文章


    authors: Niwa-Kawakita M,Miyoshi H,Gotoh O,Matsushima Y,Nishimura M,Shisa H,Ohki M

    更新日期:1995-10-10 00:00:00

  • A point mutation creating an extra N-glycosylation site in fibrillin-1 results in neonatal Marfan syndrome.

    abstract::Fibrillin-1 is a large cysteine-rich glycoprotein of the 10-nm microfibrils in the extracellular matrix. A spectrum of mutations in the fibrillin-1 gene (FBN1) have been identified in patients with Marfan syndrome (MFS), and the majority of mutations resulting in the neonatal and often lethal form of MFS have been ide...


    pub_type: 杂志文章


    authors: Lönnqvist L,Karttunen L,Rantamäki T,Kielty C,Raghunath M,Peltonen L

    更新日期:1996-09-15 00:00:00

  • Functional characterization of the human PAX3 gene regulatory region.

    abstract::Spatiotemporal expression of the PAX3 gene is tightly regulated during development. We have isolated and sequenced the 5'-flanking regulatory region of human PAX3. Primer extension and ribonuclease protection mapping revealed that transcription is initiated from a single start site downstream of a TATA-like motif in h...


    pub_type: 杂志文章


    authors: Okladnova O,Syagailo YV,Tranitz M,Riederer P,Stöber G,Mössner R,Lesch KP

    更新日期:1999-04-01 00:00:00

  • Exon-intron structure of a 2.7-kb transcript of the STM7 gene with phosphatidylinositol-4-phosphate 5-kinase activity.

    abstract::The STM7 gene encodes a novel phosphatidylinositol-4-phosphate 5-kinase (PtdInsP 5-kinase) that is subject to alternative splicing and developmental control. We have recently presented data indicating that several splice variants of STM7 incorporate elements of the X25 sequence, previously implicated in the pathogenes...


    pub_type: 杂志文章


    authors: Pook MA,Carvajal JJ,Doudney K,Hillermann R,Chamberlain S

    更新日期:1997-05-15 00:00:00

  • Prioritizing drug targets in Clostridium botulinum with a computational systems biology approach.

    abstract::A computational and in silico system level framework was developed to identify and prioritize the antibacterial drug targets in Clostridium botulinum (Clb), the causative agent of flaccid paralysis in humans that can be fatal in 5 to 10% of cases. This disease is difficult to control due to the emergence of drug-resis...


    pub_type: 杂志文章


    authors: Muhammad SA,Ahmed S,Ali A,Huang H,Wu X,Yang XF,Naz A,Chen J

    更新日期:2014-07-01 00:00:00

  • A refined physical map of the long arm of human chromosome 16.

    abstract::Mapping of 33 anonymous DNA probes and 12 genes to the long arm of chromosome 16 was achieved by the use of 14 mouse/human hybrid cell lines and the fragile site FRA16B. Two of the hybrid cell lines contained overlapping interstitial deletions in bands q21 and q22.1. The localization of the 12 genes has been refined. ...


    pub_type: 杂志文章


    authors: Chen LZ,Harris PC,Apostolou S,Baker E,Holman K,Lane SA,Nancarrow JK,Whitmore SA,Stallings RL,Hildebrand CE

    更新日期:1991-06-01 00:00:00

  • Four paralogous protein 4.1 genes map to distinct chromosomes in mouse and human.

    abstract::Four highly conserved members of the skeletal protein 4.1 gene family encode a diverse array of protein isoforms via tissue-specific transcription and developmentally regulated alternative pre-mRNA splicing. In addition to the prototypical red blood cell 4.1R (human gene symbol EPB41,) these include two homologues tha...


    pub_type: 杂志文章


    authors: Peters LL,Weier HU,Walensky LD,Snyder SH,Parra M,Mohandas N,Conboy JG

    更新日期:1998-12-01 00:00:00

  • A somatic cell hybrid panel for localizing DNA segments near the Huntington's disease gene.

    abstract::Thirty-four random DNA probes from the terminal half of the human chromosome 4 short arm were further localized within 4pter----p15.1. A panel of somatic cell hybrid lines defining six chromosomal regions within 4pter----p15.1 was constructed using human cell lines containing translocation or deletion chromosomes. The...


    pub_type: 杂志文章


    authors: MacDonald ME,Anderson MA,Gilliam TC,Tranejaerg L,Carpenter NJ,Magenis E,Hayden MR,Healey ST,Bonner TI,Gusella JF

    更新日期:1987-09-01 00:00:00

  • Mutation identification of the DSPP in a Chinese family with DGI-II and an up-to-date bioinformatic analysis.

    abstract::In this study, through linkage analysis of a four-generation Chinese family with multiple members afflicted with DGI (type II), we identified a novel missense mutation in DSPP. The mutation was located in exon 2 at the second nucleotide position of the last codon and resulted in a substitution of a proline with a leuc...


    pub_type: 杂志文章


    authors: Li D,Du X,Zhang R,Shen B,Huang Y,Valenzuela RK,Wang B,Zhao H,Liu Z,Li J,Xu Z,Gao L,Ma J

    更新日期:2012-04-01 00:00:00

  • Analysis of exon/intron structure and 400 kb of genomic sequence surrounding the 5'-promoter and 3'-terminal ends of the human glypican 3 (GPC3) gene.

    abstract::GPC3, the gene modified in the Simpson-Golabi-Behmel gigantism/overgrowth syndrome (SGBS), is shown to span more than 500 kb of genomic sequence, with the transcript beginning 197 bp 5' of the translational start site. The Xq26.1 region containing GPC3 as the only known gene has been extended to > 900 kb by sequence a...


    pub_type: 杂志文章


    authors: Huber R,Crisponi L,Mazzarella R,Chen CN,Su Y,Shizuya H,Chen EY,Cao A,Pilia G

    更新日期:1997-10-01 00:00:00

  • Construction and screening of a cosmid library generated from a somatic cell hybrid bearing human chromosome 15.

    abstract::A cosmid library has been constructed with DNA isolated from a mouse/human hybrid cell line designated A15, which was previously characterized and shown to retain chromosome 15 as the only human material. The library was generated and stored as 34 independent pools of primary colonies at 8-10,000 colonies per pool. Sc...


    pub_type: 杂志文章


    authors: McDaniel LD,Zhang B,Kubiczek E,Ritter M,Huang J,Berard C,Leana-Cox J,Schwartz S,Schultz RA

    更新日期:1997-02-15 00:00:00

  • TrioMDR: Detecting SNP interactions in trio families with model-based multifactor dimensionality reduction.

    abstract::Single nucleotide polymorphism (SNP) interactions can explain the missing heritability of common complex diseases. Many interaction detection methods have been proposed in genome-wide association studies, and they can be divided into two types: population-based and family-based. Compared with population-based methods,...


    pub_type: 杂志文章


    authors: Liu J,Yu G,Ren Y,Guo M,Wang J

    更新日期:2019-09-01 00:00:00

  • Microarray analysis of gene expression profile in resistant and susceptible Bombyx mori strains reveals resistance-related genes to nucleopolyhedrovirus.

    abstract::To investigate the molecular mechanism of silkworm resistance to BmNPV infection, we constructed a near-isogenic line (BC8) with BmNPV resistance using highly resistant (NB) and highly susceptible parental strains (306). We investigated variations in the gene expression in the midguts of BmNPV-infected BC8 and 306 at ...


    pub_type: 杂志文章


    authors: Zhou Y,Gao L,Shi H,Xia H,Gao L,Lian C,Chen L,Yao Q,Chen K,Liu X

    更新日期:2013-04-01 00:00:00

  • Differential expansion of the N-formylpeptide receptor gene cluster in human and mouse.

    abstract::The human formylpeptide receptor (FPR) gene cluster has three members: FPR1 and FPRL1, which are expressed in neutrophils and monocytes and encode seven-transmembrane-domain chemotactic receptors specific for N-formylpeptides, and FPRL2, whose function is unknown. The FPRL1 receptor is also a lipoxin A4 receptor. Usin...


    pub_type: 杂志文章


    authors: Gao JL,Chen H,Filie JD,Kozak CA,Murphy PM

    更新日期:1998-07-15 00:00:00

  • Neurturin, a novel neurotrophic factor, is localized to mouse chromosome 17 and human chromosome 19p13.3.

    abstract::Neurturin is a potent neurotrophic factor closely related to glial cell line-derived neurotrophic factor (GDNF, 40% amino acid sequence identity) and, like GDNF, can promote the survival of numerous neuronal populations including sympathetic, nodose, and dorsal root ganglion sensory neurons. Both neurturin and GDNF si...


    pub_type: 杂志文章


    authors: Heuckeroth RO,Kotzbauer P,Copeland NG,Gilbert DJ,Jenkins NA,Zimonjic DB,Popescu NC,Johnson EM Jr,Milbrandt J

    更新日期:1997-08-15 00:00:00

  • A first comparative map of copy number variations in the sheep genome.

    abstract::We carried out a cross species cattle-sheep array comparative genome hybridization experiment to identify copy number variations (CNVs) in the sheep genome analysing ewes of Italian dairy or dual-purpose breeds (Bagnolese, Comisana, Laticauda, Massese, Sarda, and Valle del Belice) using a tiling oligonucleotide array ...


    pub_type: 杂志文章


    authors: Fontanesi L,Beretti F,Martelli PL,Colombo M,Dall'olio S,Occidente M,Portolano B,Casadio R,Matassino D,Russo V

    更新日期:2011-03-01 00:00:00

  • A dicistronic gene pair within a cluster of "EF-hand" protein genes in the genomes of Drosophila species.

    abstract::Androcam is a Drosophila melanogaster calmodulin-related protein that functions specifically in the testis. We show that the Acam gene is part of a cluster of three intronless genes arranged in a head-to-tail manner. The additional genes also encode calmodulin-related proteins with testis-specific transcription. Acam ...


    pub_type: 杂志文章


    authors: Pavlik P,Konduri V,Massa E,Simonette R,Beckingham KM

    更新日期:2006-09-01 00:00:00

  • Genomic mismatch scanning identifies human genomic DNA shared identical by descent.

    abstract::Genomic mismatch scanning (GMS) is a high-throughput, high-resolution identity by descent mapping technique that enriches for genomic DNA fragments that are shared between related individuals. In GMS, DNA heteroduplexes are formed from restriction-digested genomic DNA fragments from two relatives. Mismatch-free DNA he...


    pub_type: 杂志文章


    authors: Cheung VG,Nelson SF

    更新日期:1998-01-01 00:00:00

  • Conservation of position and sequence of a novel, widely expressed gene containing the major human alpha-globin regulatory element.

    abstract::We have determined the cDNA and genomic structure of a gene (-14 gene) that lies adjacent to the human alpha-globin cluster. Although it is expressed in a wide range of cell lines and tissues, a previously described erythroid-specific regulatory element that controls expression of the alpha-globin genes lies within in...


    pub_type: 杂志文章


    authors: Vyas P,Vickers MA,Picketts DJ,Higgs DR

    更新日期:1995-10-10 00:00:00

  • Amplification of CFTR exon 9 sequences to multiple locations in the human genome.

    abstract::Cloning and characterization of the cystic fibrosis transmembrane conductance regulator (CFTR) gene led to the identification and isolation of cDNA and genomic sequences that cross-hybridized to the first nucleotide binding fold of CFTR. DNA sequence analysis of these clones showed that the cross-hybridizing sequences...


    pub_type: 杂志文章


    authors: Rozmahel R,Heng HH,Duncan AM,Shi XM,Rommens JM,Tsui LC

    更新日期:1997-11-01 00:00:00