Abstract:
:The distal end of chromosome 4q contains the locus involved in facioscapulohumeral muscular dystrophy (FSHD1). Specific genomic deletions within a tandem DNA repeat (D4Z4) are associated with the disease status, but no causal genes have yet been discovered. In a systematic search for genes, a 161-kb stretch of genomic DNA proximal to D4Z4 was sequenced, analyzed for homologies, and subjected to gene prediction programs. A major fraction (45%) of the subtelomeric region is composed of repeat sequences attributable mainly to LINE-1 elements. Apart from the previously identified FRG1 and TUB4q sequences, several additional potential coding regions were identified by analyzing the sequence with exon prediction programs. So far, we have been unable to demonstrate transcripts by RT-PCR or cDNA library hybridization. However, several retrotransposed pseudogenes were identified. The high density of pseudogenes and repeat elements is consistent with the subtelomeric location of this region and explains why previous transcript identification studies have been problematic.
journal_name
Genomicsjournal_title
Genomicsauthors
van Geel M,Heather LJ,Lyle R,Hewitt JE,Frants RR,de Jong PJdoi
10.1006/geno.1999.5942subject
Has Abstractpub_date
1999-10-01 00:00:00pages
55-65issue
1eissn
0888-7543issn
1089-8646pii
S0888-7543(99)95942-6journal_volume
61pub_type
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