Abstract:
:During growth, invasion, and metastasis, tumor cells interact extensively with the surrounding stroma. To identify genes that are upregulated during this process, we compared mRNA pooled from tumor cells and fibroblasts cultured separately to mRNA from cells in coculture. Using differential display (DD), a transcript representing a novel gene, designated epithelial-stromal interaction 1 (breast) (EPSTI1), was identified. EPSTI1 showed no homology to any known gene, but matched a cluster of expressed-sequence tags (ESTs). The full-length cDNA of 1508 bp was generated by 5'-RACE, included an open reading frame (ORF) encoding a putative 307-amino-acid protein, and mapped to chromosome 13q13.3. EPSTI1 was highly upregulated in invasive breast carcinomas compared with normal breast. In a tissue mRNA panel the most prominent expression of EPSTI1 was found in placenta. Thus, EPSTI1 is a novel human gene expressed in tissues characterized by extensive epithelial-stromal interaction, and expression of this gene may be a crucial event in invasion and metastasis of cancer.
journal_name
Genomicsjournal_title
Genomicsauthors
Nielsen HL,Rønnov-Jessen L,Villadsen R,Petersen OWdoi
10.1006/geno.2002.6755subject
Has Abstractpub_date
2002-05-01 00:00:00pages
703-10issue
5eissn
0888-7543issn
1089-8646pii
S0888754302967558journal_volume
79pub_type
杂志文章相关文献
GENOMICS文献大全abstract::Vertebrate tissue inhibitors of metalloproteinases (TIMPs) regulate extracellular matrix metalloproteinases and are thus involved in a wide variety of developmental and physiological processes. By identifying cDNAs of a transcript detected within an intron of the Drosophila synapsin gene we have cloned the Drosophila ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1999.5776
更新日期:1999-04-15 00:00:00
abstract::We have mapped the mouse protein tyrosine phosphatase epsilon (PTP epsilon, gene symbol Ptpre) gene to the distal region of chromosome 7 by linkage analysis using two sets of multilocus genetic crosses. The human PTP epsilon gene (gene symbol PTPRE) was mapped to chromosome 10q26 by fluorescence in situ hybridization....
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1996.0061
更新日期:1996-02-01 00:00:00
abstract::Homozygous transgenic mice from line A4 have an early-onset progressive neuromuscular disorder characterized by paralysis of the rear limbs, muscle atrophy, and lethality by 4 weeks of age. The transgene insertion site was mapped to distal chromosome 15 close to the locus motor endplate disease (med). The sequence of ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(95)80198-u
更新日期:1995-03-20 00:00:00
abstract::A NotI end clone library has been constructed from a human-hamster hybrid cell line containing only human chromosome 11. Fifty-one NotI clones were chosen to characterize the library. The majority of NotI clones hybridize to small 15- to 200-kb fragments and have proven to be valuable for chromosome 11 physical mappin...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1993.1120
更新日期:1993-03-01 00:00:00
abstract::We have constructed a genetic linkage map of 17 markers on the long arm of human chromosome 21, including six genes and two anonymous loci with a variable number of tandem repeats. The estimated length of the map is 103 cM in males and 140 cM in females, assuming Kosambi interference. Recombination in females was appr...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(89)90282-6
更新日期:1989-05-01 00:00:00
abstract::The frizzled (fz) locus in Drosophila is required for the transmission of polarity signals across the plasma membrane in epidermal cells, as well as to their neighboring cells in the developing wing. The identification of a tissue polarity gene from the fz locus in Drosophila melanogaster has been reported. The fz gen...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1995.1060
更新日期:1995-05-20 00:00:00
abstract::Parasites of the genus Plasmodium infect a wide array of hosts, causing malaria in all major groups of terrestrial vertebrates including primates, reptiles, and birds. Molecular mechanisms explaining why some Plasmodium species are virulent, while other closely related malaria pathogens are relatively benign in the sa...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2018.12.004
更新日期:2019-12-01 00:00:00
abstract::Human chromosome 11 harbors many genes of medical significance and cancer-related rearrangements. The availability of cloned DNA in cosmids and in yeast artificial chromosomes (YACs), combined with fluorescence in situ hybridization analysis, has led to the cloning of genes at sites of chromosomal breakpoints in acute...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1994.1354
更新日期:1994-07-01 00:00:00
abstract::Palmitoyl-protein thioesterase (PPT) is a small glycoprotein that removes palmitate groups from cysteine residues in lipid-modified proteins. We recently reported mutations in PPT in patients with infantile neuronal ceroid lipofuscinosis (INCL), a severe neurodegenerative disorder (J. Vesa et al., 1995, Nature 376: 58...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1996.0292
更新日期:1996-06-15 00:00:00
abstract::The Trp-Asp (WD) motif has been shown to exist in a number of proteins. Genes containing repeats of the WD motif compose a large gene family associated with a variety of cellular functions and can be divided into a number of functional subfamilies. By means of the differential display method using ttw, a mouse model f...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.2000.6475
更新日期:2001-03-15 00:00:00
abstract::Iduronate-2-sulfatase (IDS) is involved in the degradation of heparan sulfate and dermatan sulfate in the lysosomes, and a deficiency in this enzyme results in Hunter syndrome. A 2.3-kb cDNA clone that contains the entire coding sequence of IDS has previously been reported. Here we describe the identification of a 1.4...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1995.1249
更新日期:1995-09-01 00:00:00
abstract::The complexity of the somatic embryogenesis (SE) transcriptome suggests that numerous molecules are involved. To understand better the functional genomics of complex molecular systems during this important reprogramming process, we used bioinformatics and a pathway database to construct a draft network based on transc...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2007.07.007
更新日期:2007-11-01 00:00:00
abstract::A genetic map of nine loci defined by polymorphic DNA markers was created using a single cross of F344/N and LEW/N rats. The markers contained polymorphic simple sequence repeats identified in five genes, renin (Ren), cardiac troponin T (Tnnt3), synaptotagmin (Syt2), Na+,K(+)-ATPase catalytic subunit (Atp1a2), and the...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1993.1466
更新日期:1993-11-01 00:00:00
abstract::MSG1 (melanocyte-specific gene 1) is a recently isolated gene predominantly expressed in cultured normal melanocytes and pigmented melanoma cells. MSG1 encodes a 27-kDa nuclear protein that has strong intrinsic transcriptional transactivating activity. In this report, the human MSG1 gene was mapped to chromosome Xq13....
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1998.5383
更新日期:1998-08-01 00:00:00
abstract::CL-20 is a novel gene encoding a protein that is structurally related to but distinct from the peripheral myelin protein PMP22. Like PMP22, CL-20 is likely to play important roles in the regulation of cell proliferation, differentiation, and cell death. In this study, we describe the cloning and sequencing of a cDNA e...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1997.4524
更新日期:1997-04-01 00:00:00
abstract::The region of the dystrophin gene containing introns 45-50 is characterized by a high rate of recombination events that give rise to large deletions causing dystrophinopathy. The nucleotide sequence of this intronic region has recently been released in GenBank. With the aim of further understanding the mechanism favor...
journal_title:Genomics
pub_type: 杂志文章
doi:
更新日期:2002-11-01 00:00:00
abstract::The human CBFA2T1 (also known as MTG8) gene, on chromosome 8, has been identified through its involvement in the t(8;21) chromosomal translocation, frequently found in acute myeloid leukemia. We report here the isolation and characterization of the mouse homologue of the CBFA2T1 gene, Cbfa2t1h. Nucleotide sequence ana...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1995.9941
更新日期:1995-10-10 00:00:00
abstract::Williams syndrome (WS) is a multisystem developmental disorder caused by the deletion of contiguous genes at 7q11.23. Hemizygosity of the elastin (ELN) gene can account for the vascular and connective tissue abnormalities observed in WS patients, but the genes that contribute to features such as infantile hypercalcemi...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1996.0469
更新日期:1996-09-01 00:00:00
abstract::One of the major challenges in genome research is the identification of the complete set of genes in a genome. Alignments of expressed sequences (RNA and EST) with genomic sequences have been used to characterize genes. However, the number of alignments far exceeds the likely number of genes in a genome, suggesting th...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2003.07.003
更新日期:2004-04-01 00:00:00
abstract::The human UBE2L3 gene encodes the ubiquitin-conjugating enzyme UbcH7, demonstrated to participate in the ubiquitination of p53, c-Fos, and NF-kappaB in vitro. We report the fine-mapping of this four-exon gene to chromosome 22q11.2. We have constructed a comprehensive genomic clone contig across this gene, demonstratin...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1998.5257
更新日期:1998-07-01 00:00:00
abstract::We used targeted exon trapping to clone portions of genes from human chromosome 21q22.3. One trapped sequence showed complete homology with the cDNA of human U2AF35 (M96982; HGM-approved nomenclature U2AF1), which encodes for the small 35-kDa subunit of the U2 snRNP auxiliary factor. Using the U2AF1 cDNA as a probe, w...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1996.0196
更新日期:1996-04-15 00:00:00
abstract::We have isolated cDNA clones for the gene, termed GPX1, encoding the major human selenoprotein, glutathione peroxidase. Sequence analysis confirmed previous findings that the unusual amino acid seleno-cysteine is encoded by the opal terminator codon UGA. Southern blot analysis of human genomic DNA with the GPX1 cDNA s...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(90)90566-d
更新日期:1990-02-01 00:00:00
abstract::We have characterized three terminal deletions of the long arm of the X chromosome. Southern analysis using Xq27/q28 probes suggests that two of the deletions have breakpoints near the fragile site at Xq27.3. Flow karyotype analysis provides an estimate of 12 X 10(6) bp for the size of the deleted region. We have not ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(87)90028-0
更新日期:1987-12-01 00:00:00
abstract::The extremely high proliferation rate of tumor cells contributes to pancreatic cancer (PC) progression. Runt-related transcription factor 1(RUNX1), a key factor in hematopoiesis that was correlated with tumor progression. However, the role of RUNX1 in PC proliferation was still unclear. We found that RUNX1 was signifi...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2020.11.010
更新日期:2020-11-01 00:00:00
abstract::It is usually assumed that co-expressed genes suggest co-regulation in the underlying regulatory network. Determining sets of co-expressed genes is an important task, based on some criteria of similarity. This task is usually performed by clustering algorithms, where the genes are clustered into meaningful groups base...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2017.06.009
更新日期:2017-10-01 00:00:00
abstract::We report a genetic linkage map of the pericentromeric region of the human X chromosome, extending from Xp11 to Xq13. Genetic analysis with five polymorphic markers, including centromeric alpha satellite DNA, spanned a distance of approximately 38 cM. Significant lod scores were obtained with linkage analysis in 26 fa...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(88)90017-1
更新日期:1988-05-01 00:00:00
abstract::Point mutations in the factor VIII gene are responsible for the majority of cases of hemophilia A, and only a small fraction of these mutations can be recognized by restriction endonuclease analysis. We have now used polymerase chain reaction and denaturing gradient gel electrophoresis to characterize single nucleotid...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(90)90569-g
更新日期:1990-02-01 00:00:00
abstract::alpha-Satellite subfamilies from chromosomes 21 and 13 are almost identical in sequence and cannot be distinguished from each other by hybridization techniques. A general method based on membrane-bound PCR is described here, allowing the discrimination of alpha-satellite DNA sequences from each of these two chromosome...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/s0888-7543(05)80256-3
更新日期:1992-10-01 00:00:00
abstract::Non-coding RNA is no longer considered to be "junk" DNA, based on evidence uncovered in recent decades. In particular, the important role played by natural antisense transcripts (NATs) in regulating the expression of genes is receiving increasing attention. However, the regulatory mechanisms of NATs remain incompletel...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/j.ygeno.2018.01.011
更新日期:2019-03-01 00:00:00
abstract::As the exponential growth of DNA sequence information in databases continues, the task of converting this deposited information into knowledge becomes more dependent on integrative sequence analysis and visualization tools. PANORAMA is an Internet-accessible software package that performs a variety of informatics anal...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.2000.6359
更新日期:2000-12-15 00:00:00