Abstract:
:alpha-Satellite subfamilies from chromosomes 21 and 13 are almost identical in sequence and cannot be distinguished from each other by hybridization techniques. A general method based on membrane-bound PCR is described here, allowing the discrimination of alpha-satellite DNA sequences from each of these two chromosomes, after detection by Southern blot hybridization. The PCR conditions were developed using somatic hybrid DNAs. The method was tested in membrane-bound PCR by using the alpha-satellite bands from a Southern blot of a CEPH family. The chromosomal origin of these bands, previously determined by linkage analysis, was confirmed by this method.
journal_name
Genomicsjournal_title
Genomicsauthors
Charlieu JP,Murgue B,Laurent AM,Marçais B,Bellis M,Roizès Gdoi
10.1016/s0888-7543(05)80256-3subject
Has Abstractpub_date
1992-10-01 00:00:00pages
515-6issue
2eissn
0888-7543issn
1089-8646pii
S0888-7543(05)80256-3journal_volume
14pub_type
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