Abstract:
:Double-stranded RNA (dsRNA) is produced in host cells during viral replication. The effects of DNA demethylation on gene expression in dsRNA transfected swine cells are unclear. The study aims to profile the transcriptome changes which are induced by DNA methyltransferase inhibitor (Aza-CdR) in porcine PK15 cells transfected with viral-like dsRNA (Poly(I:C)). A total of 44, 76 and 952 differentially expressed genes (DEGs) were detected in the cells treated by Poly(I:C) plus Aza-CdR (P+A), Poly(I:C) (P) or Aza-CdR (A) alone compared to the controls (C). Immune response-related pathways are observed in the comparison of A vs. C and P vs. C, and the genes in the pathways were recovered in the comparison of (P+A) vs. C. GO analysis indicated that Aza-CdR has negative regulatory effects on viral reproduction. The results suggest that the stimulant of Poly(I:C) could be regressed by Aza-CdR. These observations provide new insights into the epigenetic regulatory effects on viral replication.
journal_name
Genomicsjournal_title
Genomicsauthors
Wang X,Ao H,Zhai L,Bai L,He W,Yu Y,Wang Cdoi
10.1016/j.ygeno.2013.10.005subject
Has Abstractpub_date
2014-05-01 00:00:00pages
371-9issue
5-6eissn
0888-7543issn
1089-8646pii
S0888-7543(13)00200-0journal_volume
103pub_type
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