Abstract:
:The transcriptional organization of the region of the mouse X chromosome between the G6pd and the Fln1 genes was studied in detail, and it was compared with the syntenic region of the human chromosome. A cosmid contig of 250 kb was constructed by screening mouse cosmid libraries with probes for human genes and with whole cosmids. Overlapping cosmids were aligned by comparing EcoRI and rare-cutter restriction enzyme digestions. The gene order and the orientation of transcription were determined by hybridization with fragments from the 5' and 3' moieties of each cDNA. Our work demonstrates that all of the new genes identified in human are present in the mouse. The size of the region, 250 kb, is also very similar, as are gene order and gene organization: the transcriptional organization in "domains" described in human is found to be identical in the mouse. The major difference detected is the much lower content in rare-cutter restriction sites, which is related to the lower G+C and CpG content of mouse DNA. The very high conservation that we have described suggests that a potent selective pressure has contributed to such conservation of gene organization.
journal_name
Genomicsjournal_title
Genomicsauthors
Rivella S,Tamanini F,Bione S,Mancini M,Herman G,Chatterjee A,Maestrini E,Toniolo Ddoi
10.1006/geno.1995.1164subject
Has Abstractpub_date
1995-08-10 00:00:00pages
377-82issue
3eissn
0888-7543issn
1089-8646pii
S0888-7543(85)71164-0journal_volume
28pub_type
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