Abstract:
:Syndactyly in Holstein cattle is an autosomal recessive abnormality characterized by the fusion of the functional digits. This disorder has been previously mapped to the telomeric part of bovine chromosome 15. Here, we describe the fine-mapping of syndactyly in Holstein cattle to a 3.5-Mb critical interval using a comparative mapping approach and an extended pedigree generated by embryo transfer. We report genetic evidence for the exclusion of two genes previously suggested as candidates (EXT2 and ALX4) and describe the identification of a doublet mutation in complete linkage disequilibrium with syndactyly in one gene of the critical interval: LRP4. Finally, based on recent discoveries concerning the mouse mutants dan and mdig and a mouse knockout for Lrp4, we present solid evidence that the subsequent substitution in LRP4 exon 33 is a strong candidate causal mutation for syndactyly in Holstein cattle.
journal_name
Genomicsjournal_title
Genomicsauthors
Duchesne A,Gautier M,Chadi S,Grohs C,Floriot S,Gallard Y,Caste G,Ducos A,Eggen Adoi
10.1016/j.ygeno.2006.05.007subject
Has Abstractpub_date
2006-11-01 00:00:00pages
610-21issue
5eissn
0888-7543issn
1089-8646pii
S0888-7543(06)00151-0journal_volume
88pub_type
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