Abstract:
:By direct sequence analysis of 94 mutant phenylalanine hydroxylase alleles using polymerase chain reaction-based techniques, we identified a C to T transition in exon 7 of the human phenylalanine hydroxylase gene that is associated with RFLP haplotypes 1 and 4. A leucine for proline substitution at position 281 can be predicted from the nucleotide sequence of the mutant codon. Expression analysis in cultured mammalian cells after site-directed mutagenesis proved that the base substitution is a disease causing gene lesion. Dot-blot hybridization analysis using allele-specific oligonucleotides revealed that 25% of all mutant haplotype 1 alleles in the German population bear this mutation. In addition, this mutation could be detected on one mutant haplotype 4 allele. The fact that this mutation is associated with only 25% of all mutant haplotype 1 alleles suggests that multiple mutations may be associated with this haplotype. The occurrence of several different mutations would be in agreement with the clinical heterogeneity observed in the group of patients whose PKU alleles belong to haplotype 1.
journal_name
Genomicsjournal_title
Genomicsauthors
Dworniczak B,Grudda K,Stümper J,Bartholomé K,Aulehla-Scholz C,Horst Jdoi
10.1016/0888-7543(91)90238-asubject
Has Abstractpub_date
1991-01-01 00:00:00pages
193-9issue
1eissn
0888-7543issn
1089-8646pii
0888-7543(91)90238-Ajournal_volume
9pub_type
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