Abstract:
:LRP1B is a novel candidate tumor suppressor gene that is inactivated by genetic and transcript alterations in nearly 50% of non-small-cell lung cancer cell lines. The gene-encoded protein is highly homologous to the gigantic lipoprotein receptor-related protein 1 (LRP1) that belongs to the family of low-density lipoprotein receptors. Using a combination of PCR-based genome walking and long-distance interexon PCR, we have determined the genomic organization of LRP1B and built a contiguous array of BAC clones spanning this gene. A total of 91 exons, varying in size from 77 bases (exon 87) to 1899 bases (exon 91), were identified in the more than 500-kb-long gene sequence. Comparative analysis of the genomic structures of LRP1B and the homologous LRP1 gene revealed a striking similarity in the location and sizes of their exons.
journal_name
Genomicsjournal_title
Genomicsauthors
Liu CX,Musco S,Lisitsina NM,Yaklichkin SY,Lisitsyn NAdoi
10.1006/geno.2000.6331subject
Has Abstractpub_date
2000-10-15 00:00:00pages
271-4issue
2eissn
0888-7543issn
1089-8646pii
S0888-7543(00)96331-6journal_volume
69pub_type
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